Main and epistatic QTL analyses for Sclerotinia Head Rot resistance in sunflower
Sclerotinia Head Rot (SHR), a disease caused by Sclerotinia sclerotiorum, is one of the most limiting factors in sunflower production. In this study, we identified genomic loci associated with resistance to SHR to support the development of assisted breeding strategies. We genotyped 114 Recombinant...
| Autores principales: | , , , , , , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
PLOS
2019
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/4550 https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0189859 https://doi.org/10.1371/journal.pone.0189859 |
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| author | Zubrzycki, Jeremias Enrique Maringolo, Carla Andrea Filippi, Carla Valeria Quiroz, Facundo Jose Nishinakamasu, Veronica Puebla, Andrea Fabiana Di Rienzo, Julio Alejandro Escande, Alberto Lia, Veronica Viviana Heinz, Ruth Amelia Hopp, Horacio Esteban Cervigni, Gerardo Domingo Lucio Paniego, Norma Beatriz |
| author_browse | Cervigni, Gerardo Domingo Lucio Di Rienzo, Julio Alejandro Escande, Alberto Filippi, Carla Valeria Heinz, Ruth Amelia Hopp, Horacio Esteban Lia, Veronica Viviana Maringolo, Carla Andrea Nishinakamasu, Veronica Paniego, Norma Beatriz Puebla, Andrea Fabiana Quiroz, Facundo Jose Zubrzycki, Jeremias Enrique |
| author_facet | Zubrzycki, Jeremias Enrique Maringolo, Carla Andrea Filippi, Carla Valeria Quiroz, Facundo Jose Nishinakamasu, Veronica Puebla, Andrea Fabiana Di Rienzo, Julio Alejandro Escande, Alberto Lia, Veronica Viviana Heinz, Ruth Amelia Hopp, Horacio Esteban Cervigni, Gerardo Domingo Lucio Paniego, Norma Beatriz |
| author_sort | Zubrzycki, Jeremias Enrique |
| collection | INTA Digital |
| description | Sclerotinia Head Rot (SHR), a disease caused by Sclerotinia sclerotiorum, is one of the most limiting factors in sunflower production. In this study, we identified genomic loci associated with resistance to SHR to support the development of assisted breeding strategies. We genotyped 114 Recombinant Inbred Lines (RILs) along with their parental lines (PAC2 –partially resistant–and RHA266 –susceptible–) by using a 384 single nucleotide polymorphism (SNP) Illumina Oligo Pool Assay to saturate a sunflower genetic map. Subsequently, we tested these lines for SHR resistance using assisted inoculations with S. sclerotiorum ascospores. We also conducted a randomized complete-block assays with three replicates to visually score disease incidence (DI), disease severity (DS), disease intensity (DInt) and incubation period (IP) through four field trials (2010–2014). We finally assessed main effect quantitative trait loci (M-QTLs) and epistatic QTLs (E-QTLs) by composite interval mapping (CIM) and mixed-model-based composite interval mapping (MCIM), respectively. As a result of this study, the improved map incorporates 61 new SNPs over candidate genes. We detected a broad range of narrow sense heritability (h2) values (1.86–59.9%) as well as 36 M-QTLs and 13 E-QTLs along 14 linkage groups (LGs). On LG1, LG10, and LG15, we repeatedly detected QTLs across field trials; which emphasizes their putative effectiveness against SHR. In all selected variables, most of the identified QTLs showed high determination coefficients, associated with moderate to high heritability values. Using markers shared with previous Sclerotinia resistance studies, we compared the QTL locations in LG1, LG2, LG8, LG10, LG11, LG15 and LG16. This study constitutes the largest report of QTLs for SHR resistance in sunflower. Further studies focusing on the regions in LG1, LG10, and LG15 harboring the detected QTLs are necessary to identify causal alleles and contribute to unraveling the complex genetic basis governing the resistance. |
| format | Artículo |
| id | INTA4550 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | PLOS |
| publisherStr | PLOS |
| record_format | dspace |
| spelling | INTA45502020-07-15T17:34:10Z Main and epistatic QTL analyses for Sclerotinia Head Rot resistance in sunflower Zubrzycki, Jeremias Enrique Maringolo, Carla Andrea Filippi, Carla Valeria Quiroz, Facundo Jose Nishinakamasu, Veronica Puebla, Andrea Fabiana Di Rienzo, Julio Alejandro Escande, Alberto Lia, Veronica Viviana Heinz, Ruth Amelia Hopp, Horacio Esteban Cervigni, Gerardo Domingo Lucio Paniego, Norma Beatriz Helianthus Annuus Sclerotinia Podredumbres Rots Girasol Sunflower Sclerotinia Head Rot Sclerotinia Head Rot (SHR), a disease caused by Sclerotinia sclerotiorum, is one of the most limiting factors in sunflower production. In this study, we identified genomic loci associated with resistance to SHR to support the development of assisted breeding strategies. We genotyped 114 Recombinant Inbred Lines (RILs) along with their parental lines (PAC2 –partially resistant–and RHA266 –susceptible–) by using a 384 single nucleotide polymorphism (SNP) Illumina Oligo Pool Assay to saturate a sunflower genetic map. Subsequently, we tested these lines for SHR resistance using assisted inoculations with S. sclerotiorum ascospores. We also conducted a randomized complete-block assays with three replicates to visually score disease incidence (DI), disease severity (DS), disease intensity (DInt) and incubation period (IP) through four field trials (2010–2014). We finally assessed main effect quantitative trait loci (M-QTLs) and epistatic QTLs (E-QTLs) by composite interval mapping (CIM) and mixed-model-based composite interval mapping (MCIM), respectively. As a result of this study, the improved map incorporates 61 new SNPs over candidate genes. We detected a broad range of narrow sense heritability (h2) values (1.86–59.9%) as well as 36 M-QTLs and 13 E-QTLs along 14 linkage groups (LGs). On LG1, LG10, and LG15, we repeatedly detected QTLs across field trials; which emphasizes their putative effectiveness against SHR. In all selected variables, most of the identified QTLs showed high determination coefficients, associated with moderate to high heritability values. Using markers shared with previous Sclerotinia resistance studies, we compared the QTL locations in LG1, LG2, LG8, LG10, LG11, LG15 and LG16. This study constitutes the largest report of QTLs for SHR resistance in sunflower. Further studies focusing on the regions in LG1, LG10, and LG15 harboring the detected QTLs are necessary to identify causal alleles and contribute to unraveling the complex genetic basis governing the resistance. Instituto de Biotecnología Fil: Zubrzycki, Jeremias Enrique. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Maringolo, Carla Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Laboratorio de Patología Vegetal; Argentina Fil: Filippi, Carla Valeria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Quiroz, Facundo Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Laboratorio de Patología Vegetal; Argentina Fil: Nishinakamasu, Veronica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Puebla, Andrea Fabiana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Di Rienzo, Julio Alejandro. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias. Cátedra de Estadística y Biometría; Argentina Fil: Escande, Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Laboratorio de Patología Vegetal; Argentina Fil: Lia, Veronica Viviana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina Fil: Heinz, Ruth Amelia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina Fil: Hopp, Horacio Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina Fil: Cervigni, Gerardo Domingo Lucio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina Fil: Paniego, Norma Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2019-03-07T17:54:19Z 2019-03-07T17:54:19Z 2017-12 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/4550 https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0189859 1932-6203 https://doi.org/10.1371/journal.pone.0189859 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf PLOS PLoS ONE 12 (12) : e0189859 (2017) |
| spellingShingle | Helianthus Annuus Sclerotinia Podredumbres Rots Girasol Sunflower Sclerotinia Head Rot Zubrzycki, Jeremias Enrique Maringolo, Carla Andrea Filippi, Carla Valeria Quiroz, Facundo Jose Nishinakamasu, Veronica Puebla, Andrea Fabiana Di Rienzo, Julio Alejandro Escande, Alberto Lia, Veronica Viviana Heinz, Ruth Amelia Hopp, Horacio Esteban Cervigni, Gerardo Domingo Lucio Paniego, Norma Beatriz Main and epistatic QTL analyses for Sclerotinia Head Rot resistance in sunflower |
| title | Main and epistatic QTL analyses for Sclerotinia Head Rot resistance in sunflower |
| title_full | Main and epistatic QTL analyses for Sclerotinia Head Rot resistance in sunflower |
| title_fullStr | Main and epistatic QTL analyses for Sclerotinia Head Rot resistance in sunflower |
| title_full_unstemmed | Main and epistatic QTL analyses for Sclerotinia Head Rot resistance in sunflower |
| title_short | Main and epistatic QTL analyses for Sclerotinia Head Rot resistance in sunflower |
| title_sort | main and epistatic qtl analyses for sclerotinia head rot resistance in sunflower |
| topic | Helianthus Annuus Sclerotinia Podredumbres Rots Girasol Sunflower Sclerotinia Head Rot |
| url | http://hdl.handle.net/20.500.12123/4550 https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0189859 https://doi.org/10.1371/journal.pone.0189859 |
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