Development of an APC-targeted multivalent E2-based vaccine against Bovine Viral Diarrhea Virus types 1 and 2
The aim of this study was to develop and test a multivalent subunit vaccine against Bovine Viral Diarrhea Virus (BVDV) based on the E2 virus glycoprotein belonging to genotypes 1a, 1b and 2a, immunopotentiated by targeting these antigens to antigen-presenting cells. The E2 antigens were expressed in...
| Autores principales: | , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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Elsevier
2019
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/4536 https://www.sciencedirect.com/science/article/pii/S0264410X15011147?via%3Dihub https://doi.org/10.1016/j.vaccine.2015.07.106 |
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| author | Pecora, Andrea Malacari, Darío Amilcar Pérez Aguirreburualde, María Sol Bellido, Demian Nuñez, Maria del Carmen Dus Santos, Maria Jose Martínez Escribano, José Angel Wigdorovitz, Andres |
| author_browse | Bellido, Demian Dus Santos, Maria Jose Malacari, Darío Amilcar Martínez Escribano, José Angel Nuñez, Maria del Carmen Pecora, Andrea Pérez Aguirreburualde, María Sol Wigdorovitz, Andres |
| author_facet | Pecora, Andrea Malacari, Darío Amilcar Pérez Aguirreburualde, María Sol Bellido, Demian Nuñez, Maria del Carmen Dus Santos, Maria Jose Martínez Escribano, José Angel Wigdorovitz, Andres |
| author_sort | Pecora, Andrea |
| collection | INTA Digital |
| description | The aim of this study was to develop and test a multivalent subunit vaccine against Bovine Viral Diarrhea Virus (BVDV) based on the E2 virus glycoprotein belonging to genotypes 1a, 1b and 2a, immunopotentiated by targeting these antigens to antigen-presenting cells. The E2 antigens were expressed in insect cells by a baculovirus vector as fusion proteins with a single chain antibody, named APCH I, which recognizes the β-chain of the MHC Class II antigen. The three chimeric proteins were evaluated for their immunogenicity in a guinea pig model as well as in colostrum-deprived calves. Once the immune response in experimentally vaccinated calves was evaluated, immunized animals were challenged with type 1b or type 2b BVDV in order to study the protection conferred by the experimental vaccine.
The recombinant APCH I-tE21a-1b-2a vaccine was immunogenic both in guinea pigs and calves, inducing neutralizing antibodies. After BVDV type 1b and type 2 challenge of vaccinated calves in a proof of concept, the type 1b virus could not be isolated in any animal; meanwhile it was detected in all challenged non-vaccinated control animals. However, the type 2 BVDV was isolated to a lesser extent compared to unvaccinated animals challenged with type 2 BVDV. Clinical signs associated to BVDV, hyperthermia and leukopenia were reduced with respect to controls in all vaccinated calves. Given these results, this multivalent vaccine holds promise for a safe and effective tool to control BVDV in herds |
| format | Artículo |
| id | INTA4536 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA45362019-03-01T18:59:23Z Development of an APC-targeted multivalent E2-based vaccine against Bovine Viral Diarrhea Virus types 1 and 2 Pecora, Andrea Malacari, Darío Amilcar Pérez Aguirreburualde, María Sol Bellido, Demian Nuñez, Maria del Carmen Dus Santos, Maria Jose Martínez Escribano, José Angel Wigdorovitz, Andres Pestivirus de la Diarrea Bovina Bovine Diarrhoea Pestivirus Glycoproteins Glicoproteínas Polyvalent Vaccines Vacuna Polivalente Vaccines Vacuna E2 Glycoprotein BVDV The aim of this study was to develop and test a multivalent subunit vaccine against Bovine Viral Diarrhea Virus (BVDV) based on the E2 virus glycoprotein belonging to genotypes 1a, 1b and 2a, immunopotentiated by targeting these antigens to antigen-presenting cells. The E2 antigens were expressed in insect cells by a baculovirus vector as fusion proteins with a single chain antibody, named APCH I, which recognizes the β-chain of the MHC Class II antigen. The three chimeric proteins were evaluated for their immunogenicity in a guinea pig model as well as in colostrum-deprived calves. Once the immune response in experimentally vaccinated calves was evaluated, immunized animals were challenged with type 1b or type 2b BVDV in order to study the protection conferred by the experimental vaccine. The recombinant APCH I-tE21a-1b-2a vaccine was immunogenic both in guinea pigs and calves, inducing neutralizing antibodies. After BVDV type 1b and type 2 challenge of vaccinated calves in a proof of concept, the type 1b virus could not be isolated in any animal; meanwhile it was detected in all challenged non-vaccinated control animals. However, the type 2 BVDV was isolated to a lesser extent compared to unvaccinated animals challenged with type 2 BVDV. Clinical signs associated to BVDV, hyperthermia and leukopenia were reduced with respect to controls in all vaccinated calves. Given these results, this multivalent vaccine holds promise for a safe and effective tool to control BVDV in herds Instituto de Virología Fil: Pecora, Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Malacari, Darío Amilcar. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Pérez Aguirreburualde, María Sol. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Bellido, Demian. Vetanco SA; Argentina Fil: Nuñez, Maria del Carmen. Algenex SL, Madrid; España Fil: Dus Santos, Maria Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Martínez Escribano, José Angel. Instituto Nacional de Investigación Agraria. Departamento de Biotecnología; España Fil: Wigdorovitz, Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2019-03-01T18:56:08Z 2019-03-01T18:56:08Z 2015-09 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/4536 https://www.sciencedirect.com/science/article/pii/S0264410X15011147?via%3Dihub 0264-410X https://doi.org/10.1016/j.vaccine.2015.07.106 eng info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Vaccine 33 (39) : 5163-5171. (22 September 2015) |
| spellingShingle | Pestivirus de la Diarrea Bovina Bovine Diarrhoea Pestivirus Glycoproteins Glicoproteínas Polyvalent Vaccines Vacuna Polivalente Vaccines Vacuna E2 Glycoprotein BVDV Pecora, Andrea Malacari, Darío Amilcar Pérez Aguirreburualde, María Sol Bellido, Demian Nuñez, Maria del Carmen Dus Santos, Maria Jose Martínez Escribano, José Angel Wigdorovitz, Andres Development of an APC-targeted multivalent E2-based vaccine against Bovine Viral Diarrhea Virus types 1 and 2 |
| title | Development of an APC-targeted multivalent E2-based vaccine against Bovine Viral Diarrhea Virus types 1 and 2 |
| title_full | Development of an APC-targeted multivalent E2-based vaccine against Bovine Viral Diarrhea Virus types 1 and 2 |
| title_fullStr | Development of an APC-targeted multivalent E2-based vaccine against Bovine Viral Diarrhea Virus types 1 and 2 |
| title_full_unstemmed | Development of an APC-targeted multivalent E2-based vaccine against Bovine Viral Diarrhea Virus types 1 and 2 |
| title_short | Development of an APC-targeted multivalent E2-based vaccine against Bovine Viral Diarrhea Virus types 1 and 2 |
| title_sort | development of an apc targeted multivalent e2 based vaccine against bovine viral diarrhea virus types 1 and 2 |
| topic | Pestivirus de la Diarrea Bovina Bovine Diarrhoea Pestivirus Glycoproteins Glicoproteínas Polyvalent Vaccines Vacuna Polivalente Vaccines Vacuna E2 Glycoprotein BVDV |
| url | http://hdl.handle.net/20.500.12123/4536 https://www.sciencedirect.com/science/article/pii/S0264410X15011147?via%3Dihub https://doi.org/10.1016/j.vaccine.2015.07.106 |
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