Foot-and-mouth disease virus infection of dendritic cells triggers phosphorylation of ERK1/2 inducing class I presentation and apoptosis
Foot-and-mouth disease (FMD) is a highly contagious viral disease of cloven-hoofed animals. This pathology is caused by foot-and-mouth disease virus (FMDV). Over time, the development of vaccines to prevent the spread of this illness became essential. Vaccines currently used contain the inactivated...
| Autores principales: | , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2019
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/4535 https://www.sciencedirect.com/science/article/pii/S0264410X15009858?via%3Dihub https://doi.org/10.1016/j.vaccine.2015.07.038 |
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| author | Langellotti, Cecilia Ana Cesar, Gonzalo Soria, Ivana Quattrocchi, Valeria Jancic, Carolina Cristina Zamorano, Patricia Ines Vermeulen, Elba Monica |
| author_browse | Cesar, Gonzalo Jancic, Carolina Cristina Langellotti, Cecilia Ana Quattrocchi, Valeria Soria, Ivana Vermeulen, Elba Monica Zamorano, Patricia Ines |
| author_facet | Langellotti, Cecilia Ana Cesar, Gonzalo Soria, Ivana Quattrocchi, Valeria Jancic, Carolina Cristina Zamorano, Patricia Ines Vermeulen, Elba Monica |
| author_sort | Langellotti, Cecilia Ana |
| collection | INTA Digital |
| description | Foot-and-mouth disease (FMD) is a highly contagious viral disease of cloven-hoofed animals. This pathology is caused by foot-and-mouth disease virus (FMDV). Over time, the development of vaccines to prevent the spread of this illness became essential. Vaccines currently used contain the inactivated form of the virus. However, vaccination generates an immune response different to that induced by the infection. We investigated whether these differences are related to intracellular mechanisms on dendritic cells (DCs). As a result, we demonstrated that the internalization of infective virus triggered the phosphorylation of ERK1/2, which was involved in the activation of caspase-9, the intrinsic pathway of apoptosis and the delivery of viral peptides on MHC class I molecules. While, inactivated virus (iFMDV) did not affect this pathway or any function mediated by its activation. As described, infectious virus in DCs was also associated to autophagy LC3 protein and was associated to lysosomal protein Lamp-2; contrary to observe for the iFMDV. Strikingly, the processing of viral antigens to accommodate in class I molecules does not appear to involve the proteasome. Finally, this increased presentation promotes a specific cytotoxic response against infectious virus. |
| format | Artículo |
| id | INTA4535 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA45352019-03-01T18:01:05Z Foot-and-mouth disease virus infection of dendritic cells triggers phosphorylation of ERK1/2 inducing class I presentation and apoptosis Langellotti, Cecilia Ana Cesar, Gonzalo Soria, Ivana Quattrocchi, Valeria Jancic, Carolina Cristina Zamorano, Patricia Ines Vermeulen, Elba Monica Aphthovirus Phosphorylation Cytotoxicity Virus Fiebre Aftosa Fosforilación Citotoxicidad Foot and Mouth Disease Virus Caspase-9 Inactive Viral Particles ERK1/2 MAPK Células Dendríticas Foot-and-mouth disease (FMD) is a highly contagious viral disease of cloven-hoofed animals. This pathology is caused by foot-and-mouth disease virus (FMDV). Over time, the development of vaccines to prevent the spread of this illness became essential. Vaccines currently used contain the inactivated form of the virus. However, vaccination generates an immune response different to that induced by the infection. We investigated whether these differences are related to intracellular mechanisms on dendritic cells (DCs). As a result, we demonstrated that the internalization of infective virus triggered the phosphorylation of ERK1/2, which was involved in the activation of caspase-9, the intrinsic pathway of apoptosis and the delivery of viral peptides on MHC class I molecules. While, inactivated virus (iFMDV) did not affect this pathway or any function mediated by its activation. As described, infectious virus in DCs was also associated to autophagy LC3 protein and was associated to lysosomal protein Lamp-2; contrary to observe for the iFMDV. Strikingly, the processing of viral antigens to accommodate in class I molecules does not appear to involve the proteasome. Finally, this increased presentation promotes a specific cytotoxic response against infectious virus. Instituto de Virología Fil: Langellotti, Cecilia Ana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Cesar, Gonzalo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina Fil: Soria, Ivana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Quattrocchi, Valeria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Jancic, Carolina Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina Fil: Zamorano, Patricia Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Vermeulen, Elba Monica. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina 2019-03-01T17:54:39Z 2019-03-01T17:54:39Z 2015-09 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/4535 https://www.sciencedirect.com/science/article/pii/S0264410X15009858?via%3Dihub 0264-410X https://doi.org/10.1016/j.vaccine.2015.07.038 eng info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Vaccine 33 (38) : 4945-4953. (11 September 2015) |
| spellingShingle | Aphthovirus Phosphorylation Cytotoxicity Virus Fiebre Aftosa Fosforilación Citotoxicidad Foot and Mouth Disease Virus Caspase-9 Inactive Viral Particles ERK1/2 MAPK Células Dendríticas Langellotti, Cecilia Ana Cesar, Gonzalo Soria, Ivana Quattrocchi, Valeria Jancic, Carolina Cristina Zamorano, Patricia Ines Vermeulen, Elba Monica Foot-and-mouth disease virus infection of dendritic cells triggers phosphorylation of ERK1/2 inducing class I presentation and apoptosis |
| title | Foot-and-mouth disease virus infection of dendritic cells triggers phosphorylation of ERK1/2 inducing class I presentation and apoptosis |
| title_full | Foot-and-mouth disease virus infection of dendritic cells triggers phosphorylation of ERK1/2 inducing class I presentation and apoptosis |
| title_fullStr | Foot-and-mouth disease virus infection of dendritic cells triggers phosphorylation of ERK1/2 inducing class I presentation and apoptosis |
| title_full_unstemmed | Foot-and-mouth disease virus infection of dendritic cells triggers phosphorylation of ERK1/2 inducing class I presentation and apoptosis |
| title_short | Foot-and-mouth disease virus infection of dendritic cells triggers phosphorylation of ERK1/2 inducing class I presentation and apoptosis |
| title_sort | foot and mouth disease virus infection of dendritic cells triggers phosphorylation of erk1 2 inducing class i presentation and apoptosis |
| topic | Aphthovirus Phosphorylation Cytotoxicity Virus Fiebre Aftosa Fosforilación Citotoxicidad Foot and Mouth Disease Virus Caspase-9 Inactive Viral Particles ERK1/2 MAPK Células Dendríticas |
| url | http://hdl.handle.net/20.500.12123/4535 https://www.sciencedirect.com/science/article/pii/S0264410X15009858?via%3Dihub https://doi.org/10.1016/j.vaccine.2015.07.038 |
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