Characterization of the two component regulatory system PhoPR in Mycobacterium bovis
Mycobacterium bovis is the causative agent of bovine tuberculosis and is a member of Mycobacterium tuberculosis complex, which causes tuberculosis in a number of mammals including humans. Previous studies have shown that the genes encoding the two-component system PhoPR, which regulates several ge...
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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Elsevier
2019
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| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.12123/4531 https://www.sciencedirect.com/science/article/pii/S0378113518300725?via%3Dihub https://doi.org/10.1016/j.vetmic.2018.06.016 |
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| author | Garcia, Elizabeth Andrea Blanco, Federico Carlos Bigi, María Mercedes Vazquez, Cristina Lourdes Forrellad, Marina Andrea Rocha, Rosana Valeria Golby, Paul Soria, Marcelo Abel Bigi, Fabiana |
| author_browse | Bigi, Fabiana Bigi, María Mercedes Blanco, Federico Carlos Forrellad, Marina Andrea Garcia, Elizabeth Andrea Golby, Paul Rocha, Rosana Valeria Soria, Marcelo Abel Vazquez, Cristina Lourdes |
| author_facet | Garcia, Elizabeth Andrea Blanco, Federico Carlos Bigi, María Mercedes Vazquez, Cristina Lourdes Forrellad, Marina Andrea Rocha, Rosana Valeria Golby, Paul Soria, Marcelo Abel Bigi, Fabiana |
| author_sort | Garcia, Elizabeth Andrea |
| collection | INTA Digital |
| description | Mycobacterium bovis is the causative agent of bovine tuberculosis and is a member of Mycobacterium tuberculosis
complex, which causes tuberculosis in a number of mammals including humans. Previous studies have shown
that the genes encoding the two-component system PhoPR, which regulates several genes involved in the
virulence of M. tuberculosis, are polymorphic in M. bovis, when compared to M. tuberculosis, which results in a
dysfunctional two-component system. In this study we investigated the role of PhoPR in two M. bovis strains with
differing degrees of virulence. We found that the deletion of phoP in an M. bovis isolate reduced its capacity of
inducing phagosomal arrest in bovine macrophages. By gene expression analysis, we demonstrated that, in both
M. bovis strains, PhoP regulates the expression of a putative lipid desaturase Mb1404-Mb1405, a protein involved
in redox stress AhpC, the sulfolipid transporter Mmpl8 and the secreted antigen ESAT-6. Furthermore, the
lack of PhoP increased the sensitivity to acidic stress and alteration of the biofilm/pellicle formation of M. bovis.
Both these phenotypes are connected to bacterial redox homeostasis. Therefore, the results of this study suggest a
role of PhoPR in M. bovis to be linked to the mechanisms that mycobacteria display to maintain their redox
balance. |
| format | info:ar-repo/semantics/artículo |
| id | INTA4531 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA45312019-03-01T15:14:55Z Characterization of the two component regulatory system PhoPR in Mycobacterium bovis Garcia, Elizabeth Andrea Blanco, Federico Carlos Bigi, María Mercedes Vazquez, Cristina Lourdes Forrellad, Marina Andrea Rocha, Rosana Valeria Golby, Paul Soria, Marcelo Abel Bigi, Fabiana Mycobacterium Bovis Expresión Génica Components Gene Expression PhoPR Mycobacterium bovis is the causative agent of bovine tuberculosis and is a member of Mycobacterium tuberculosis complex, which causes tuberculosis in a number of mammals including humans. Previous studies have shown that the genes encoding the two-component system PhoPR, which regulates several genes involved in the virulence of M. tuberculosis, are polymorphic in M. bovis, when compared to M. tuberculosis, which results in a dysfunctional two-component system. In this study we investigated the role of PhoPR in two M. bovis strains with differing degrees of virulence. We found that the deletion of phoP in an M. bovis isolate reduced its capacity of inducing phagosomal arrest in bovine macrophages. By gene expression analysis, we demonstrated that, in both M. bovis strains, PhoP regulates the expression of a putative lipid desaturase Mb1404-Mb1405, a protein involved in redox stress AhpC, the sulfolipid transporter Mmpl8 and the secreted antigen ESAT-6. Furthermore, the lack of PhoP increased the sensitivity to acidic stress and alteration of the biofilm/pellicle formation of M. bovis. Both these phenotypes are connected to bacterial redox homeostasis. Therefore, the results of this study suggest a role of PhoPR in M. bovis to be linked to the mechanisms that mycobacteria display to maintain their redox balance. Instituto de Biotecnología Fil: Garcia, Elizabeth Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Blanco, Federico Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Bigi, María Mercedes. Universidad de Buenos Aires. Facultad de Agronomía, Cátedra de Microbiología Agrícola; Argentina. . Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; Argentina Fil: Vazquez, Cristina Lourdes. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Forrellad, Marina Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Rocha, Rosana Valeria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Golby, Paul. CN Bio Innovations, Welwyn Garden City; Reino Unido Fil: Soria, Marcelo Abel. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Biología Aplicada y Alimentos. Cátedra de Microbiología Agrícola; Argentina Fil: Bigi, Fabiana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina 2019-03-01T14:49:02Z 2019-03-01T14:49:02Z 2018-08 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/4531 https://www.sciencedirect.com/science/article/pii/S0378113518300725?via%3Dihub 0378-1135 https://doi.org/10.1016/j.vetmic.2018.06.016 eng info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Veterinary microbiology 222 : 30-38. (August 2018) |
| spellingShingle | Mycobacterium Bovis Expresión Génica Components Gene Expression PhoPR Garcia, Elizabeth Andrea Blanco, Federico Carlos Bigi, María Mercedes Vazquez, Cristina Lourdes Forrellad, Marina Andrea Rocha, Rosana Valeria Golby, Paul Soria, Marcelo Abel Bigi, Fabiana Characterization of the two component regulatory system PhoPR in Mycobacterium bovis |
| title | Characterization of the two component regulatory system PhoPR in Mycobacterium bovis |
| title_full | Characterization of the two component regulatory system PhoPR in Mycobacterium bovis |
| title_fullStr | Characterization of the two component regulatory system PhoPR in Mycobacterium bovis |
| title_full_unstemmed | Characterization of the two component regulatory system PhoPR in Mycobacterium bovis |
| title_short | Characterization of the two component regulatory system PhoPR in Mycobacterium bovis |
| title_sort | characterization of the two component regulatory system phopr in mycobacterium bovis |
| topic | Mycobacterium Bovis Expresión Génica Components Gene Expression PhoPR |
| url | http://hdl.handle.net/20.500.12123/4531 https://www.sciencedirect.com/science/article/pii/S0378113518300725?via%3Dihub https://doi.org/10.1016/j.vetmic.2018.06.016 |
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