On-site detection of equid alphaherpesvirus 3 in perineal and genital swabs of mares and stallions
Equine coital exanthema (ECE) is an infectious, venereally transmitted muco-cutaneous disease affecting mares and stallions, caused by equid alphaherpesvirus 3 (EHV3). Diagnostic tools for rapid identification of EHV3 are of primary importance to diminish the risk of EHV3 dissemination at the time o...
| Autores principales: | , , , , , , , , |
|---|---|
| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2019
|
| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/4479 https://www.sciencedirect.com/science/article/pii/S0166093418300260?via%3Dihub https://doi.org/10.1016/j.jviromet.2018.04.002 |
| _version_ | 1855035330912059392 |
|---|---|
| author | Vissani, Maria Aldana Tordoya, Maria Silvia Tsai, Yun-Long Lee, Pei-Yu Alison Shen, Yu-Han Lee, Fu-Chun Wang, Hwa-Tang Thomas Parreño, Viviana Barrandeguy, Maria Edith |
| author_browse | Barrandeguy, Maria Edith Lee, Fu-Chun Lee, Pei-Yu Alison Parreño, Viviana Shen, Yu-Han Tordoya, Maria Silvia Tsai, Yun-Long Vissani, Maria Aldana Wang, Hwa-Tang Thomas |
| author_facet | Vissani, Maria Aldana Tordoya, Maria Silvia Tsai, Yun-Long Lee, Pei-Yu Alison Shen, Yu-Han Lee, Fu-Chun Wang, Hwa-Tang Thomas Parreño, Viviana Barrandeguy, Maria Edith |
| author_sort | Vissani, Maria Aldana |
| collection | INTA Digital |
| description | Equine coital exanthema (ECE) is an infectious, venereally transmitted muco-cutaneous disease affecting mares and stallions, caused by equid alphaherpesvirus 3 (EHV3). Diagnostic tools for rapid identification of EHV3 are of primary importance to diminish the risk of EHV3 dissemination at the time of breeding. In the last years, it has been shown that the performance of the insulated-isothermal polymerase chain reaction (iiPCR) is comparable to virus isolation, nested PCR and real-time PCR (qPCR) in detecting pathogens of various animal species. Analytical sensitivity and specificity of the iiPCR were compared with a qPCR, using a plasmid containing the target region of the EHV3 glycoprotein G gene and an Argentinian EHV3 isolate (E/9283/07 C3A). In order to evaluate the diagnostic performance of the iiPCR, nucleic acids of 85 perineal and genital swabs (PGS) of mares and stallions were extracted by tacoTM mini and tested by both techniques. EHV3 was detected in 46 and 45 of the 85 PGS by the iiPCR and qPCR, respectively. There was almost perfect agreement between the two diagnostic methods (98.82%; 95% CI: 95.03–100%; κ = 0.98). The iiPCR had a limit of detection of 95.00% at 6 genome equivalents per reaction and a detection endpoint for viral DNA comparable to that of the qPCR, and did not react with six non-targeted equine pathogens. The iiPCR represents a sensitive and specific method for the rapid on-site diagnosis of EHV3 infection. Its routinely implementation in breeding facilities, and artificial insemination and embryo transfer centers, will contribute to prevent the dissemination of this venereal, highly contagious disease in horses. |
| format | info:ar-repo/semantics/artículo |
| id | INTA4479 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA44792023-04-12T16:08:50Z On-site detection of equid alphaherpesvirus 3 in perineal and genital swabs of mares and stallions Vissani, Maria Aldana Tordoya, Maria Silvia Tsai, Yun-Long Lee, Pei-Yu Alison Shen, Yu-Han Lee, Fu-Chun Wang, Hwa-Tang Thomas Parreño, Viviana Barrandeguy, Maria Edith Herpesviridae Exantema Coital Yegua Animal Reproductor PCR Coital Exanthema Mares Breeding Sbtock Equid Herpesvirus Detección In Situ Equinos Equine Coital Exanthema Insulated-isothermal Polymerase Chain Reaction On-site Detection Equine coital exanthema (ECE) is an infectious, venereally transmitted muco-cutaneous disease affecting mares and stallions, caused by equid alphaherpesvirus 3 (EHV3). Diagnostic tools for rapid identification of EHV3 are of primary importance to diminish the risk of EHV3 dissemination at the time of breeding. In the last years, it has been shown that the performance of the insulated-isothermal polymerase chain reaction (iiPCR) is comparable to virus isolation, nested PCR and real-time PCR (qPCR) in detecting pathogens of various animal species. Analytical sensitivity and specificity of the iiPCR were compared with a qPCR, using a plasmid containing the target region of the EHV3 glycoprotein G gene and an Argentinian EHV3 isolate (E/9283/07 C3A). In order to evaluate the diagnostic performance of the iiPCR, nucleic acids of 85 perineal and genital swabs (PGS) of mares and stallions were extracted by tacoTM mini and tested by both techniques. EHV3 was detected in 46 and 45 of the 85 PGS by the iiPCR and qPCR, respectively. There was almost perfect agreement between the two diagnostic methods (98.82%; 95% CI: 95.03–100%; κ = 0.98). The iiPCR had a limit of detection of 95.00% at 6 genome equivalents per reaction and a detection endpoint for viral DNA comparable to that of the qPCR, and did not react with six non-targeted equine pathogens. The iiPCR represents a sensitive and specific method for the rapid on-site diagnosis of EHV3 infection. Its routinely implementation in breeding facilities, and artificial insemination and embryo transfer centers, will contribute to prevent the dissemination of this venereal, highly contagious disease in horses. Instituto de Virología Fil: Vissani, Aldana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Tordoya, Maria Silvia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Tsai, Yun-Long. GeneReach USA, Lexington; Estados Unidos Fil: Lee, Pei-Yu Alison. GeneReach USA, Lexington; Estados Unidos Fil: Shen, Yu-Han. GeneReach USA, Lexington; Estados Unidos Fil: Lee, Fu-Chun. GeneReach USA, Lexington; Estados Unidos Fil: Wang, Hwa-Tang Thomas. GeneReach USA, Lexington; Estados Unidos Fil: Parreño, Viviana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Barrandeguy, Maria Edith. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Universidad del Salvador. Escuela de Veterinaria. Cátedra de Enfermedades Infecciosas; Argentina 2019-02-21T13:01:52Z 2019-02-21T13:01:52Z 2018-07 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/4479 https://www.sciencedirect.com/science/article/pii/S0166093418300260?via%3Dihub 0166-0934 https://doi.org/10.1016/j.jviromet.2018.04.002 eng info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Journal of virological methods 257 : 29-32. (July 2018) |
| spellingShingle | Herpesviridae Exantema Coital Yegua Animal Reproductor PCR Coital Exanthema Mares Breeding Sbtock Equid Herpesvirus Detección In Situ Equinos Equine Coital Exanthema Insulated-isothermal Polymerase Chain Reaction On-site Detection Vissani, Maria Aldana Tordoya, Maria Silvia Tsai, Yun-Long Lee, Pei-Yu Alison Shen, Yu-Han Lee, Fu-Chun Wang, Hwa-Tang Thomas Parreño, Viviana Barrandeguy, Maria Edith On-site detection of equid alphaherpesvirus 3 in perineal and genital swabs of mares and stallions |
| title | On-site detection of equid alphaherpesvirus 3 in perineal and genital swabs of mares and stallions |
| title_full | On-site detection of equid alphaherpesvirus 3 in perineal and genital swabs of mares and stallions |
| title_fullStr | On-site detection of equid alphaherpesvirus 3 in perineal and genital swabs of mares and stallions |
| title_full_unstemmed | On-site detection of equid alphaherpesvirus 3 in perineal and genital swabs of mares and stallions |
| title_short | On-site detection of equid alphaherpesvirus 3 in perineal and genital swabs of mares and stallions |
| title_sort | on site detection of equid alphaherpesvirus 3 in perineal and genital swabs of mares and stallions |
| topic | Herpesviridae Exantema Coital Yegua Animal Reproductor PCR Coital Exanthema Mares Breeding Sbtock Equid Herpesvirus Detección In Situ Equinos Equine Coital Exanthema Insulated-isothermal Polymerase Chain Reaction On-site Detection |
| url | http://hdl.handle.net/20.500.12123/4479 https://www.sciencedirect.com/science/article/pii/S0166093418300260?via%3Dihub https://doi.org/10.1016/j.jviromet.2018.04.002 |
| work_keys_str_mv | AT vissanimariaaldana onsitedetectionofequidalphaherpesvirus3inperinealandgenitalswabsofmaresandstallions AT tordoyamariasilvia onsitedetectionofequidalphaherpesvirus3inperinealandgenitalswabsofmaresandstallions AT tsaiyunlong onsitedetectionofequidalphaherpesvirus3inperinealandgenitalswabsofmaresandstallions AT leepeiyualison onsitedetectionofequidalphaherpesvirus3inperinealandgenitalswabsofmaresandstallions AT shenyuhan onsitedetectionofequidalphaherpesvirus3inperinealandgenitalswabsofmaresandstallions AT leefuchun onsitedetectionofequidalphaherpesvirus3inperinealandgenitalswabsofmaresandstallions AT wanghwatangthomas onsitedetectionofequidalphaherpesvirus3inperinealandgenitalswabsofmaresandstallions AT parrenoviviana onsitedetectionofequidalphaherpesvirus3inperinealandgenitalswabsofmaresandstallions AT barrandeguymariaedith onsitedetectionofequidalphaherpesvirus3inperinealandgenitalswabsofmaresandstallions |