Novel Effector Protein EspY3 of Type III Secretion System from Enterohemorrhagic Escherichia coli Is Localized in Actin Pedestals
Enterohemorrhagic Escherichia coli (EHEC) and enteropathogenic Escherichia coli (EPEC) are attaching and effacing (A/E) pathogens, which translocate effector proteins to intestinal enterocytes through a type III secretion system (T3SS). T3SS and most of its effector proteins are encoded in a pathoge...
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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MDPI AG
2019
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| Online Access: | http://hdl.handle.net/20.500.12123/4473 https://www.mdpi.com/2076-2607/6/4/112 https://doi.org/10.3390/microorganisms6040112 |
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| author | Larzabal, Mariano Marques Da Silva, Wanderson Riviere, Nahuel Agustín Cataldi, Angel Adrian |
| author_browse | Cataldi, Angel Adrian Larzabal, Mariano Marques Da Silva, Wanderson Riviere, Nahuel Agustín |
| author_facet | Larzabal, Mariano Marques Da Silva, Wanderson Riviere, Nahuel Agustín Cataldi, Angel Adrian |
| author_sort | Larzabal, Mariano |
| collection | INTA Digital |
| description | Enterohemorrhagic Escherichia coli (EHEC) and enteropathogenic Escherichia coli (EPEC) are attaching and effacing (A/E) pathogens, which translocate effector proteins to intestinal enterocytes through a type III secretion system (T3SS). T3SS and most of its effector proteins are encoded in a pathogenicity island called LEE. Recently, new effectors have been located outside the LEE. This study aimed to characterize EspY3, a novel non-LEE encoded T3SS effector of EHEC. EspY3 shares homology with SopD and PipB2 effector proteins of Salmonella’s T3SS-1 and T3SS-2, respectively. The presence of recombinant EspY3 in the supernatant samples demonstrated that EspY3 was secreted by the T3SS of EHEC and EPEC. Through infection assays, we demonstrated the translocation of EspY3 into Caco-2 cells by T3SS of EPEC. The subcellular localization of EspY3 was determined in the pedestal region, where its presence generates a significant increase in the size of the pedestals area. The EspY3 effector induced the elongation of polymerized actin pedestals in infected Caco-2 by EPEC. This study confirmed that EspY3 is part of the repertoire of T3SS effectors of EHEC O157:H7, and that it participates in modeling cellular actin during the infection |
| format | info:ar-repo/semantics/artículo |
| id | INTA4473 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | MDPI AG |
| publisherStr | MDPI AG |
| record_format | dspace |
| spelling | INTA44732019-02-20T13:21:39Z Novel Effector Protein EspY3 of Type III Secretion System from Enterohemorrhagic Escherichia coli Is Localized in Actin Pedestals Larzabal, Mariano Marques Da Silva, Wanderson Riviere, Nahuel Agustín Cataldi, Angel Adrian Escherichia Coli Actina Polimerización Efectores Moleculares Proteínas Actin Polymerization Effectors Proteins EspY3 T3SS Type III Secretion System Enterohemorrhagic Escherichia coli (EHEC) and enteropathogenic Escherichia coli (EPEC) are attaching and effacing (A/E) pathogens, which translocate effector proteins to intestinal enterocytes through a type III secretion system (T3SS). T3SS and most of its effector proteins are encoded in a pathogenicity island called LEE. Recently, new effectors have been located outside the LEE. This study aimed to characterize EspY3, a novel non-LEE encoded T3SS effector of EHEC. EspY3 shares homology with SopD and PipB2 effector proteins of Salmonella’s T3SS-1 and T3SS-2, respectively. The presence of recombinant EspY3 in the supernatant samples demonstrated that EspY3 was secreted by the T3SS of EHEC and EPEC. Through infection assays, we demonstrated the translocation of EspY3 into Caco-2 cells by T3SS of EPEC. The subcellular localization of EspY3 was determined in the pedestal region, where its presence generates a significant increase in the size of the pedestals area. The EspY3 effector induced the elongation of polymerized actin pedestals in infected Caco-2 by EPEC. This study confirmed that EspY3 is part of the repertoire of T3SS effectors of EHEC O157:H7, and that it participates in modeling cellular actin during the infection Instituto de Biotecnología Fil: Larzabal, Mariano. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Marques Da Silva, Wanderson. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Riviere, Nahuel Agustín. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina 2019-02-20T13:17:23Z 2019-02-20T13:17:23Z 2018 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/4473 https://www.mdpi.com/2076-2607/6/4/112 2076-2607 https://doi.org/10.3390/microorganisms6040112 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf MDPI AG Microorganisms 6 (4) : 112. (2018) |
| spellingShingle | Escherichia Coli Actina Polimerización Efectores Moleculares Proteínas Actin Polymerization Effectors Proteins EspY3 T3SS Type III Secretion System Larzabal, Mariano Marques Da Silva, Wanderson Riviere, Nahuel Agustín Cataldi, Angel Adrian Novel Effector Protein EspY3 of Type III Secretion System from Enterohemorrhagic Escherichia coli Is Localized in Actin Pedestals |
| title | Novel Effector Protein EspY3 of Type III Secretion System from Enterohemorrhagic Escherichia coli Is Localized in Actin Pedestals |
| title_full | Novel Effector Protein EspY3 of Type III Secretion System from Enterohemorrhagic Escherichia coli Is Localized in Actin Pedestals |
| title_fullStr | Novel Effector Protein EspY3 of Type III Secretion System from Enterohemorrhagic Escherichia coli Is Localized in Actin Pedestals |
| title_full_unstemmed | Novel Effector Protein EspY3 of Type III Secretion System from Enterohemorrhagic Escherichia coli Is Localized in Actin Pedestals |
| title_short | Novel Effector Protein EspY3 of Type III Secretion System from Enterohemorrhagic Escherichia coli Is Localized in Actin Pedestals |
| title_sort | novel effector protein espy3 of type iii secretion system from enterohemorrhagic escherichia coli is localized in actin pedestals |
| topic | Escherichia Coli Actina Polimerización Efectores Moleculares Proteínas Actin Polymerization Effectors Proteins EspY3 T3SS Type III Secretion System |
| url | http://hdl.handle.net/20.500.12123/4473 https://www.mdpi.com/2076-2607/6/4/112 https://doi.org/10.3390/microorganisms6040112 |
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