Baculovirus capsid display in vaccination schemes: effect of a previous immunity against the vector on the cytotoxic response to delivered antigens
The baculovirus Autographa californica multiple nuclear polyhedrosis virus (AcMNPV) infects lepidopteran invertebrates as natural hosts, although it also has been used as display vector for vaccine development. In this work, we evaluated the effectiveness of repetitive doses of AcMNPV-based vectors...
| Autores principales: | , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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Springer
2019
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/4467 https://doi.org/10.1007/s00253-018-9368-8 |
| _version_ | 1855483438437498880 |
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| author | Molinari, Maria Paula Molina, Guido Nicolás Tavarone, Maria Eugenia Del Medico Zajac, Maria Paula Moron, Victor Gabriel Taboga, Oscar Alberto |
| author_browse | Del Medico Zajac, Maria Paula Molina, Guido Nicolás Molinari, Maria Paula Moron, Victor Gabriel Taboga, Oscar Alberto Tavarone, Maria Eugenia |
| author_facet | Molinari, Maria Paula Molina, Guido Nicolás Tavarone, Maria Eugenia Del Medico Zajac, Maria Paula Moron, Victor Gabriel Taboga, Oscar Alberto |
| author_sort | Molinari, Maria Paula |
| collection | INTA Digital |
| description | The baculovirus Autographa californica multiple nuclear polyhedrosis virus (AcMNPV) infects lepidopteran invertebrates as natural hosts, although it also has been used as display vector for vaccine development. In this work, we evaluated the effectiveness of repetitive doses of AcMNPV-based vectors on the cytotoxic immune response specific to the capsid-displayed heterologous antigen ovalbumin (OVA). Our results demonstrate that baculovirus vectors induce a boosting effect in the cytotoxic immune response to OVA, making possible to recover the levels obtained in the primary response. Moreover, mice preimmunized with wild-type baculovirus showed a complete lack of antigen-specific CD8 cytotoxic T lymphocytes (CTLs) that may be related to the presence of antibodies directed to baculoviral surface proteins, particularly to GP64. However, baculovirus was able to induce the innate immune response in spite of a previous response against this vector, although some quantitative differences reflect a distinct activation of the immune cells in prime and boost. This is the first report in which the novel capsid display strategy is evaluated in prime-boost schemes to improve efficient CTL responses. |
| format | Artículo |
| id | INTA4467 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA44672019-02-19T15:17:25Z Baculovirus capsid display in vaccination schemes: effect of a previous immunity against the vector on the cytotoxic response to delivered antigens Molinari, Maria Paula Molina, Guido Nicolás Tavarone, Maria Eugenia Del Medico Zajac, Maria Paula Moron, Victor Gabriel Taboga, Oscar Alberto Baculovirus Cytotoxicity Citotoxicidad Immune Response Respuesta Inmunológica Repetitive Doses Capsid Display The baculovirus Autographa californica multiple nuclear polyhedrosis virus (AcMNPV) infects lepidopteran invertebrates as natural hosts, although it also has been used as display vector for vaccine development. In this work, we evaluated the effectiveness of repetitive doses of AcMNPV-based vectors on the cytotoxic immune response specific to the capsid-displayed heterologous antigen ovalbumin (OVA). Our results demonstrate that baculovirus vectors induce a boosting effect in the cytotoxic immune response to OVA, making possible to recover the levels obtained in the primary response. Moreover, mice preimmunized with wild-type baculovirus showed a complete lack of antigen-specific CD8 cytotoxic T lymphocytes (CTLs) that may be related to the presence of antibodies directed to baculoviral surface proteins, particularly to GP64. However, baculovirus was able to induce the innate immune response in spite of a previous response against this vector, although some quantitative differences reflect a distinct activation of the immune cells in prime and boost. This is the first report in which the novel capsid display strategy is evaluated in prime-boost schemes to improve efficient CTL responses. Instituto de Biotecnología Fil: Molinari, Maria Paula. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Molina, Guido Nicolás. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Tavarone, Maria Eugenia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Del Medico Zajac, Maria Paula. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Moron, Victor Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina Fil: Taboga, Oscar Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2019-02-19T15:14:17Z 2019-02-19T15:14:17Z 2018-12 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/4467 0175-7598 1432-0614 (Online) https://doi.org/10.1007/s00253-018-9368-8 eng info:eu-repo/semantics/restrictedAccess application/pdf Springer Applied microbiology and biotechnology 102 (23) : 10139–10146. December 2018 |
| spellingShingle | Baculovirus Cytotoxicity Citotoxicidad Immune Response Respuesta Inmunológica Repetitive Doses Capsid Display Molinari, Maria Paula Molina, Guido Nicolás Tavarone, Maria Eugenia Del Medico Zajac, Maria Paula Moron, Victor Gabriel Taboga, Oscar Alberto Baculovirus capsid display in vaccination schemes: effect of a previous immunity against the vector on the cytotoxic response to delivered antigens |
| title | Baculovirus capsid display in vaccination schemes: effect of a previous immunity against the vector on the cytotoxic response to delivered antigens |
| title_full | Baculovirus capsid display in vaccination schemes: effect of a previous immunity against the vector on the cytotoxic response to delivered antigens |
| title_fullStr | Baculovirus capsid display in vaccination schemes: effect of a previous immunity against the vector on the cytotoxic response to delivered antigens |
| title_full_unstemmed | Baculovirus capsid display in vaccination schemes: effect of a previous immunity against the vector on the cytotoxic response to delivered antigens |
| title_short | Baculovirus capsid display in vaccination schemes: effect of a previous immunity against the vector on the cytotoxic response to delivered antigens |
| title_sort | baculovirus capsid display in vaccination schemes effect of a previous immunity against the vector on the cytotoxic response to delivered antigens |
| topic | Baculovirus Cytotoxicity Citotoxicidad Immune Response Respuesta Inmunológica Repetitive Doses Capsid Display |
| url | http://hdl.handle.net/20.500.12123/4467 https://doi.org/10.1007/s00253-018-9368-8 |
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