Effect of the interaction between Anticarsia gemmatalis multiple nucleopolyhedrovirus and Epinotia aporema granulovirus, on A. gemmatalis (Lepidoptera: Noctuidae) larvae
The bean shoot borer Epinotia aporema Wals. (Lepidoptera: Tortricidae) and the velvet bean caterpillar Anticarsia gemmatalis Hübner (Lepidoptera: Noctuidae) are key pests of soybean and other legume crops in South America. They are often found simultaneously in certain regions. A. gemmatalis nucle...
| Autores principales: | , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
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Elsevier
2019
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/4466 https://www.sciencedirect.com/science/article/pii/S1049964415300116?via%3Dihub https://doi.org/10.1016/j.biocontrol.2015.07.006 |
| _version_ | 1855035328529694720 |
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| author | Biedma, Marina Elizabeth Salvador, Ricardo Ferrelli, Maria Leticia Sciocco, Alicia Ines Romanowski, Víctor |
| author_browse | Biedma, Marina Elizabeth Ferrelli, Maria Leticia Romanowski, Víctor Salvador, Ricardo Sciocco, Alicia Ines |
| author_facet | Biedma, Marina Elizabeth Salvador, Ricardo Ferrelli, Maria Leticia Sciocco, Alicia Ines Romanowski, Víctor |
| author_sort | Biedma, Marina Elizabeth |
| collection | INTA Digital |
| description | The bean shoot borer Epinotia aporema Wals. (Lepidoptera: Tortricidae) and the velvet bean caterpillar
Anticarsia gemmatalis Hübner (Lepidoptera: Noctuidae) are key pests of soybean and other legume crops
in South America. They are often found simultaneously in certain regions. A. gemmatalis nucleopolyhedrovirus
(AgMNPV) is widely used to control A. gemmatalis. More recently, E. aporema granulovirus
(EpapGV) has been characterized and evaluated as a bioinsecticide for E. aporema. In order to increase
its potential use and to design optimized strategies for the management of lepidopteran pests, we evaluated
the interaction between EpapGV and AgMNPV on third instar A. gemmatalis larvae. Larvae fed with
50 AgMNPV OBs/larva showed an increase in the mortality rates (from 42% to 81%) and a decrease
in the median survival time (from 7.7 days to 5.7 days) when these OBs were mixed with
6000 EpapGV OBs/larva. When 300 AgMNPV OBs/larva were used alone or in combination with EpapGV
OBs no changes in biological parameters were observed. No mortality was detected in A. gemmatalis larvae
treated with EpapGV alone. In larvae fed with the viral mixtures, only AgMNPV DNA was detected by PCR.
A. gemmatalis peritrophic membranes (PMs) examined by SDS–PAGE and scanning electron microscopy
showed signs of damage. Notably, we found the presence of spheroidal bodies associated with damaged
areas in the PMs of larvae fed with EpapGV but not in those that were given AgMNPV alone. These results
show that EpapGV increases the viral potency of AgMNPV, and thus the insecticidal efficiency, suggesting
that the use of formulations including both viruses might be a valuable tool for pest management. |
| format | info:ar-repo/semantics/artículo |
| id | INTA4466 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA44662020-08-14T13:00:58Z Effect of the interaction between Anticarsia gemmatalis multiple nucleopolyhedrovirus and Epinotia aporema granulovirus, on A. gemmatalis (Lepidoptera: Noctuidae) larvae Biedma, Marina Elizabeth Salvador, Ricardo Ferrelli, Maria Leticia Sciocco, Alicia Ines Romanowski, Víctor Epinotia Aporema Control Biológico soja Glycine Max Biological Control Soybeans Granulovirus Membrana Peritrófica Anticarsia Gemmatalis Nucleopolyhedrovirus Peritrophic Membrane The bean shoot borer Epinotia aporema Wals. (Lepidoptera: Tortricidae) and the velvet bean caterpillar Anticarsia gemmatalis Hübner (Lepidoptera: Noctuidae) are key pests of soybean and other legume crops in South America. They are often found simultaneously in certain regions. A. gemmatalis nucleopolyhedrovirus (AgMNPV) is widely used to control A. gemmatalis. More recently, E. aporema granulovirus (EpapGV) has been characterized and evaluated as a bioinsecticide for E. aporema. In order to increase its potential use and to design optimized strategies for the management of lepidopteran pests, we evaluated the interaction between EpapGV and AgMNPV on third instar A. gemmatalis larvae. Larvae fed with 50 AgMNPV OBs/larva showed an increase in the mortality rates (from 42% to 81%) and a decrease in the median survival time (from 7.7 days to 5.7 days) when these OBs were mixed with 6000 EpapGV OBs/larva. When 300 AgMNPV OBs/larva were used alone or in combination with EpapGV OBs no changes in biological parameters were observed. No mortality was detected in A. gemmatalis larvae treated with EpapGV alone. In larvae fed with the viral mixtures, only AgMNPV DNA was detected by PCR. A. gemmatalis peritrophic membranes (PMs) examined by SDS–PAGE and scanning electron microscopy showed signs of damage. Notably, we found the presence of spheroidal bodies associated with damaged areas in the PMs of larvae fed with EpapGV but not in those that were given AgMNPV alone. These results show that EpapGV increases the viral potency of AgMNPV, and thus the insecticidal efficiency, suggesting that the use of formulations including both viruses might be a valuable tool for pest management. Instituto de Biotecnología Fil: Biedma, Marina Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina Fil: Salvador, Ricardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina Fil: Ferrelli, Maria Leticia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina Fil: Sciocco, Alicia Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina Fil: Romanowski, Víctor. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina 2019-02-19T14:38:04Z 2019-02-19T14:38:04Z 2015-12 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/4466 https://www.sciencedirect.com/science/article/pii/S1049964415300116?via%3Dihub 1049-9644 https://doi.org/10.1016/j.biocontrol.2015.07.006 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Elsevier Biological control 91 : 17-21. (December 2015) |
| spellingShingle | Epinotia Aporema Control Biológico soja Glycine Max Biological Control Soybeans Granulovirus Membrana Peritrófica Anticarsia Gemmatalis Nucleopolyhedrovirus Peritrophic Membrane Biedma, Marina Elizabeth Salvador, Ricardo Ferrelli, Maria Leticia Sciocco, Alicia Ines Romanowski, Víctor Effect of the interaction between Anticarsia gemmatalis multiple nucleopolyhedrovirus and Epinotia aporema granulovirus, on A. gemmatalis (Lepidoptera: Noctuidae) larvae |
| title | Effect of the interaction between Anticarsia gemmatalis multiple nucleopolyhedrovirus and Epinotia aporema granulovirus, on A. gemmatalis (Lepidoptera: Noctuidae) larvae |
| title_full | Effect of the interaction between Anticarsia gemmatalis multiple nucleopolyhedrovirus and Epinotia aporema granulovirus, on A. gemmatalis (Lepidoptera: Noctuidae) larvae |
| title_fullStr | Effect of the interaction between Anticarsia gemmatalis multiple nucleopolyhedrovirus and Epinotia aporema granulovirus, on A. gemmatalis (Lepidoptera: Noctuidae) larvae |
| title_full_unstemmed | Effect of the interaction between Anticarsia gemmatalis multiple nucleopolyhedrovirus and Epinotia aporema granulovirus, on A. gemmatalis (Lepidoptera: Noctuidae) larvae |
| title_short | Effect of the interaction between Anticarsia gemmatalis multiple nucleopolyhedrovirus and Epinotia aporema granulovirus, on A. gemmatalis (Lepidoptera: Noctuidae) larvae |
| title_sort | effect of the interaction between anticarsia gemmatalis multiple nucleopolyhedrovirus and epinotia aporema granulovirus on a gemmatalis lepidoptera noctuidae larvae |
| topic | Epinotia Aporema Control Biológico soja Glycine Max Biological Control Soybeans Granulovirus Membrana Peritrófica Anticarsia Gemmatalis Nucleopolyhedrovirus Peritrophic Membrane |
| url | http://hdl.handle.net/20.500.12123/4466 https://www.sciencedirect.com/science/article/pii/S1049964415300116?via%3Dihub https://doi.org/10.1016/j.biocontrol.2015.07.006 |
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