Biotechnological applications of occlusion bodies of Baculoviruses
The ability of Baculoviruses to hyper-express very late genes as polyhedrin, the major component of occlusion bodies (OBs) or polyhedra, has allowed the evolution of a system of great utility for biotechnology. The main function of polyhedra in nature is to protect Baculovirus in the environment. Th...
| Autores principales: | , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Springer
2019
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/4456 https://doi.org/10.1007/s00253-018-9130-2 |
| _version_ | 1855483436389629952 |
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| author | Lopez, Maria Gabriela Diez, Michay Alfonso, Victoria Taboga, Oscar Alberto |
| author_browse | Alfonso, Victoria Diez, Michay Lopez, Maria Gabriela Taboga, Oscar Alberto |
| author_facet | Lopez, Maria Gabriela Diez, Michay Alfonso, Victoria Taboga, Oscar Alberto |
| author_sort | Lopez, Maria Gabriela |
| collection | INTA Digital |
| description | The ability of Baculoviruses to hyper-express very late genes as polyhedrin, the major component of occlusion bodies (OBs) or polyhedra, has allowed the evolution of a system of great utility for biotechnology. The main function of polyhedra in nature is to protect Baculovirus in the environment. The possibility of incorporating foreign proteins into the crystal by fusing them to polyhedrin (POLH) opened novel potential biotechnological uses. In this review, we summarize different applications of Baculovirus chimeric OBs. Basically, the improvement of protein expression and purification with POLH as a fusion partner; the use of recombinant polyhedra as immunogens and antigens, and the incorporation of proteins into polyhedra to improve Baculoviruses as bioinsecticides. The results obtained in each area and the future trends in these topics are also discussed. |
| format | Artículo |
| id | INTA4456 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA44562019-02-18T13:33:01Z Biotechnological applications of occlusion bodies of Baculoviruses Lopez, Maria Gabriela Diez, Michay Alfonso, Victoria Taboga, Oscar Alberto Baculovirus Proteínas Recombinantes Biotecnología Recombinant Proteins Biotechnology Occlusion Bodies BEVS Polyhedrin Cuerpos de Oclusión Polihedrina The ability of Baculoviruses to hyper-express very late genes as polyhedrin, the major component of occlusion bodies (OBs) or polyhedra, has allowed the evolution of a system of great utility for biotechnology. The main function of polyhedra in nature is to protect Baculovirus in the environment. The possibility of incorporating foreign proteins into the crystal by fusing them to polyhedrin (POLH) opened novel potential biotechnological uses. In this review, we summarize different applications of Baculovirus chimeric OBs. Basically, the improvement of protein expression and purification with POLH as a fusion partner; the use of recombinant polyhedra as immunogens and antigens, and the incorporation of proteins into polyhedra to improve Baculoviruses as bioinsecticides. The results obtained in each area and the future trends in these topics are also discussed. Instituto de Biotecnología Fil: Lopez, Maria Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Diez, Michay. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Alfonso, Victoria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Taboga, Oscar Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2019-02-18T13:15:59Z 2019-02-18T13:15:59Z 2018-08 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/4456 0175-7598 1432-0614 (Online) https://doi.org/10.1007/s00253-018-9130-2 eng info:eu-repo/semantics/restrictedAccess application/pdf Springer Applied microbiology and biotechnology 102 (16) : 6765–6774. (August 2018) |
| spellingShingle | Baculovirus Proteínas Recombinantes Biotecnología Recombinant Proteins Biotechnology Occlusion Bodies BEVS Polyhedrin Cuerpos de Oclusión Polihedrina Lopez, Maria Gabriela Diez, Michay Alfonso, Victoria Taboga, Oscar Alberto Biotechnological applications of occlusion bodies of Baculoviruses |
| title | Biotechnological applications of occlusion bodies of Baculoviruses |
| title_full | Biotechnological applications of occlusion bodies of Baculoviruses |
| title_fullStr | Biotechnological applications of occlusion bodies of Baculoviruses |
| title_full_unstemmed | Biotechnological applications of occlusion bodies of Baculoviruses |
| title_short | Biotechnological applications of occlusion bodies of Baculoviruses |
| title_sort | biotechnological applications of occlusion bodies of baculoviruses |
| topic | Baculovirus Proteínas Recombinantes Biotecnología Recombinant Proteins Biotechnology Occlusion Bodies BEVS Polyhedrin Cuerpos de Oclusión Polihedrina |
| url | http://hdl.handle.net/20.500.12123/4456 https://doi.org/10.1007/s00253-018-9130-2 |
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