Two distinctive phenotypes of AcMNPV display different immune abilities and intracellular destiny
The budded phenotype (BV) of the baculovirus AcMNPV has been demonstrated to have strong immunostimulatory properties that are relevant for the development of vaccines and antiviral therapies. Although the occluded phenotype (ODV) shares the main structural proteins and its genome with BV, it has...
| Autores principales: | , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
2019
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/4455 https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0168939 https://doi.org/10.1371/journal.pone.0168939 |
| _version_ | 1855035326505943040 |
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| author | Molina, Guido Nicolas Tavarone, Maria Eugenia Taboga, Oscar Alberto Molinari, Maria Paula |
| author_browse | Molina, Guido Nicolas Molinari, Maria Paula Taboga, Oscar Alberto Tavarone, Maria Eugenia |
| author_facet | Molina, Guido Nicolas Tavarone, Maria Eugenia Taboga, Oscar Alberto Molinari, Maria Paula |
| author_sort | Molina, Guido Nicolas |
| collection | INTA Digital |
| description | The budded phenotype (BV) of the baculovirus AcMNPV has been demonstrated to have
strong immunostimulatory properties that are relevant for the development of vaccines and
antiviral therapies. Although the occluded phenotype (ODV) shares the main structural proteins
and its genome with BV, it has been poorly studied in mammals. In this study, we
assessed the capacity of ODV to induce immune responses in mice. In contrast to BVs,
ODVs failed to promote the secretion of IFN-gamma, IL-6 and Il-12 and to induce antiviral
activity against VSV in the short term. Furthermore, ODVs were unable to induce cellular
immunity against a coadministered antigen 7 days after inoculation. By analyzing the interaction
of ODVs with BMDCs, we observed that although ODVs entered the cells reaching
late and acidic endosomes, they did not induce their maturation. Finally, we also analyzed if
BVs and ODVs followed different routes in the cell during the infection. BVs, but not ODVs,
colocalized with the protein ovalbumin in compartments with the presence of proteases. The
results suggest that structural differences could be responsible for their different destinies in
the dendritic cell and this could lead to a different impact on the immune response |
| format | info:ar-repo/semantics/artículo |
| id | INTA4455 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| record_format | dspace |
| spelling | INTA44552019-02-18T12:48:37Z Two distinctive phenotypes of AcMNPV display different immune abilities and intracellular destiny Molina, Guido Nicolas Tavarone, Maria Eugenia Taboga, Oscar Alberto Molinari, Maria Paula Baculovirus Fenotipos Respuesta Inmunológica Phenotypes Immune Response AcMNPV ODV Occluded Phenotype Cuerpos de Oclusión The budded phenotype (BV) of the baculovirus AcMNPV has been demonstrated to have strong immunostimulatory properties that are relevant for the development of vaccines and antiviral therapies. Although the occluded phenotype (ODV) shares the main structural proteins and its genome with BV, it has been poorly studied in mammals. In this study, we assessed the capacity of ODV to induce immune responses in mice. In contrast to BVs, ODVs failed to promote the secretion of IFN-gamma, IL-6 and Il-12 and to induce antiviral activity against VSV in the short term. Furthermore, ODVs were unable to induce cellular immunity against a coadministered antigen 7 days after inoculation. By analyzing the interaction of ODVs with BMDCs, we observed that although ODVs entered the cells reaching late and acidic endosomes, they did not induce their maturation. Finally, we also analyzed if BVs and ODVs followed different routes in the cell during the infection. BVs, but not ODVs, colocalized with the protein ovalbumin in compartments with the presence of proteases. The results suggest that structural differences could be responsible for their different destinies in the dendritic cell and this could lead to a different impact on the immune response Instituto de Biotecnología Fil: Molina, Guido Nicolas. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Tavarone, Maria Eugenia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Taboga, Oscar Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Molinari, Maria Paula. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2019-02-18T12:40:46Z 2019-02-18T12:40:46Z 2016-12-29 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/4455 https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0168939 1932-6203 https://doi.org/10.1371/journal.pone.0168939 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf PLoS ONE 11(12) : e0168939. (2016) |
| spellingShingle | Baculovirus Fenotipos Respuesta Inmunológica Phenotypes Immune Response AcMNPV ODV Occluded Phenotype Cuerpos de Oclusión Molina, Guido Nicolas Tavarone, Maria Eugenia Taboga, Oscar Alberto Molinari, Maria Paula Two distinctive phenotypes of AcMNPV display different immune abilities and intracellular destiny |
| title | Two distinctive phenotypes of AcMNPV display different immune abilities and intracellular destiny |
| title_full | Two distinctive phenotypes of AcMNPV display different immune abilities and intracellular destiny |
| title_fullStr | Two distinctive phenotypes of AcMNPV display different immune abilities and intracellular destiny |
| title_full_unstemmed | Two distinctive phenotypes of AcMNPV display different immune abilities and intracellular destiny |
| title_short | Two distinctive phenotypes of AcMNPV display different immune abilities and intracellular destiny |
| title_sort | two distinctive phenotypes of acmnpv display different immune abilities and intracellular destiny |
| topic | Baculovirus Fenotipos Respuesta Inmunológica Phenotypes Immune Response AcMNPV ODV Occluded Phenotype Cuerpos de Oclusión |
| url | http://hdl.handle.net/20.500.12123/4455 https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0168939 https://doi.org/10.1371/journal.pone.0168939 |
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