Minimally processed crude leaf extracts of Nicotiana benthamiana containing recombinant foot and mouth disease virus-like particles are immunogenic in mice
Foot-and-mouth disease (FMD) remains one of the most feared viral diseases affecting cloven-hoofed animals, and results in severe economic losses. Currently available vaccines are based on inactivated FMD virus (FMDV). The use of recombinant FMDV-like particles (VLPs) as subunit vaccines has gained...
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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Elsevier
2019
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| Online Access: | http://hdl.handle.net/20.500.12123/4443 https://www.sciencedirect.com/science/article/pii/S2215017X1830050X?via%3Dihub https://doi.org/10.1016/j.btre.2018.e00283 |
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| author | Ruiz, Vanesa Baztarrica, Josefina Rybicki, Edward P. Meyers, Ann E. Wigdorovitz, Andres |
| author_browse | Baztarrica, Josefina Meyers, Ann E. Ruiz, Vanesa Rybicki, Edward P. Wigdorovitz, Andres |
| author_facet | Ruiz, Vanesa Baztarrica, Josefina Rybicki, Edward P. Meyers, Ann E. Wigdorovitz, Andres |
| author_sort | Ruiz, Vanesa |
| collection | INTA Digital |
| description | Foot-and-mouth disease (FMD) remains one of the most feared viral diseases affecting cloven-hoofed animals, and results in severe economic losses. Currently available vaccines are based on inactivated FMD virus (FMDV). The use of recombinant FMDV-like particles (VLPs) as subunit vaccines has gained importance because of their immunogenic properties and safety. We evaluated the production of FMD VLPs, via Agrobacterium-mediated transient expression, and the immunogenicity of these structures in mice. Leaves were infiltrated with pEAQ-HT and pRIC 3.0 vectors encoding the capsid precursor P1-2A and the protease 3C. The recombinant protein yield was 3–4 mg/kg of fresh leaf tissue. Both groups of mice immunized with purified VLPs and mice immunized with the crude leaf extract elicited a specific humoral response with similar antibody titers. Thus, minimally processed plant material containing transiently expressed FMD VLPs could be a scalable and cost-effective technology for the production of a recombinant subunit vaccine against FMDV. |
| format | info:ar-repo/semantics/artículo |
| id | INTA4443 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA44432019-02-14T15:14:37Z Minimally processed crude leaf extracts of Nicotiana benthamiana containing recombinant foot and mouth disease virus-like particles are immunogenic in mice Ruiz, Vanesa Baztarrica, Josefina Rybicki, Edward P. Meyers, Ann E. Wigdorovitz, Andres Extractos de Hoja Virus Fiebre Aftosa Inmunogenética Ratón Leaf Extracts Aphthovirus immunogenetics Mice Nicotiana Benthamiana Foot and Mouth Disease Virus Foot-and-mouth disease (FMD) remains one of the most feared viral diseases affecting cloven-hoofed animals, and results in severe economic losses. Currently available vaccines are based on inactivated FMD virus (FMDV). The use of recombinant FMDV-like particles (VLPs) as subunit vaccines has gained importance because of their immunogenic properties and safety. We evaluated the production of FMD VLPs, via Agrobacterium-mediated transient expression, and the immunogenicity of these structures in mice. Leaves were infiltrated with pEAQ-HT and pRIC 3.0 vectors encoding the capsid precursor P1-2A and the protease 3C. The recombinant protein yield was 3–4 mg/kg of fresh leaf tissue. Both groups of mice immunized with purified VLPs and mice immunized with the crude leaf extract elicited a specific humoral response with similar antibody titers. Thus, minimally processed plant material containing transiently expressed FMD VLPs could be a scalable and cost-effective technology for the production of a recombinant subunit vaccine against FMDV. Instituto de Virología Fil: Ruiz, Vanesa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Tecnológicas; Argentina Fil: Baztarrica, Josefina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Rybicki, Edward P. University of Cape Town. Department of Molecular and cell Biology. Biopharming Research Unit; Sudáfrica. University of Cape Town. Faculty of Health Sciences. Institute of Infectious Disease and Molecular Medicine; Sudáfrica Fil: Meyers, Ann E. University of Cape Town. Department of Molecular and cell Biology. Biopharming Research Unit; Sudáfrica Fil: Wigdorovitz, Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2019-02-14T15:10:30Z 2019-02-14T15:10:30Z 2018-12 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/4443 https://www.sciencedirect.com/science/article/pii/S2215017X1830050X?via%3Dihub 2215-017X https://doi.org/10.1016/j.btre.2018.e00283 eng info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Biotechnology reports 20 : e00283. (December 2018) |
| spellingShingle | Extractos de Hoja Virus Fiebre Aftosa Inmunogenética Ratón Leaf Extracts Aphthovirus immunogenetics Mice Nicotiana Benthamiana Foot and Mouth Disease Virus Ruiz, Vanesa Baztarrica, Josefina Rybicki, Edward P. Meyers, Ann E. Wigdorovitz, Andres Minimally processed crude leaf extracts of Nicotiana benthamiana containing recombinant foot and mouth disease virus-like particles are immunogenic in mice |
| title | Minimally processed crude leaf extracts of Nicotiana benthamiana containing recombinant foot and mouth disease virus-like particles are immunogenic in mice |
| title_full | Minimally processed crude leaf extracts of Nicotiana benthamiana containing recombinant foot and mouth disease virus-like particles are immunogenic in mice |
| title_fullStr | Minimally processed crude leaf extracts of Nicotiana benthamiana containing recombinant foot and mouth disease virus-like particles are immunogenic in mice |
| title_full_unstemmed | Minimally processed crude leaf extracts of Nicotiana benthamiana containing recombinant foot and mouth disease virus-like particles are immunogenic in mice |
| title_short | Minimally processed crude leaf extracts of Nicotiana benthamiana containing recombinant foot and mouth disease virus-like particles are immunogenic in mice |
| title_sort | minimally processed crude leaf extracts of nicotiana benthamiana containing recombinant foot and mouth disease virus like particles are immunogenic in mice |
| topic | Extractos de Hoja Virus Fiebre Aftosa Inmunogenética Ratón Leaf Extracts Aphthovirus immunogenetics Mice Nicotiana Benthamiana Foot and Mouth Disease Virus |
| url | http://hdl.handle.net/20.500.12123/4443 https://www.sciencedirect.com/science/article/pii/S2215017X1830050X?via%3Dihub https://doi.org/10.1016/j.btre.2018.e00283 |
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