Evaluation of the transcriptional status of host cytokines and viral genes in the trachea of vaccinated and non-vaccinated chickens after challenge with the infectious laryngotracheitis virus
Infectious laryngotracheitis is a highly contagious disease of chickens responsible for significant economic losses for the poultry industry worldwide. The disease is caused by Gallid herpesvirus-1 (GaHV-1) commonly known as the infectious laryngotracheitis virus. Although characterized by their...
| Autores principales: | , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Taylor & Francis
2019
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/4438 http://dx.doi.org/10.1080/03079457.2015.1126804 |
| _version_ | 1855483433130655744 |
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| author | Vagnozzi, Ariel Eduardo Riblet, Sylva Zavala, Guillermo Ecco, Roselene Afonso, Claudio L. García, Maricarmen |
| author_browse | Afonso, Claudio L. Ecco, Roselene García, Maricarmen Riblet, Sylva Vagnozzi, Ariel Eduardo Zavala, Guillermo |
| author_facet | Vagnozzi, Ariel Eduardo Riblet, Sylva Zavala, Guillermo Ecco, Roselene Afonso, Claudio L. García, Maricarmen |
| author_sort | Vagnozzi, Ariel Eduardo |
| collection | INTA Digital |
| description | Infectious laryngotracheitis is a highly contagious disease of chickens responsible for significant
economic losses for the poultry industry worldwide. The disease is caused by Gallid herpesvirus-1
(GaHV-1) commonly known as the infectious laryngotracheitis virus. Although characterized by
their potential to regain virulence, chicken embryo origin (CEO) vaccines are the most effective
vaccines against laryngotracheitis as they significantly reduce the replication of challenge virus
in the trachea and conjunctiva. Knowledge on the nature of protective immunity elicited by CEO
vaccines is very limited. Therefore, elucidating the origin of the immune responses elicited by
CEO vaccination is relevant for development of safer control strategies. In this study the
transcription levels of key host immune genes (IFN-γ, IFN-β, IL-1β, IL-6, IL-8, IL-18) and viral
genes (ICP4, ICP27, UL46, UL49), as well as viral genome loads in trachea were quantified at
6 and 12 hours post-challenge of CEO vaccinated and non-vaccinated chickens. Immediately
after challenge a significant increase in IFN-γ gene expression was followed by a significant
reduction in viral replication. In contrast to the rapid induction of IFN-γ, expression of the
pro-inflammatory cytokines (IL-1β, IL-6, IL-8) and type I IFN β was either slightly reduced or
remained at basal levels. These suggest that the former cytokines may not play important
roles during immediate early responses induced by ILTV challenge in either vaccinated or
non-vaccinated chickens. Overall, these results suggest that the rapid expression of IFN-γ
may induce pathways of antiviral responses necessary for blocking early virus replication. |
| format | Artículo |
| id | INTA4438 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Taylor & Francis |
| publisherStr | Taylor & Francis |
| record_format | dspace |
| spelling | INTA44382019-02-13T18:26:20Z Evaluation of the transcriptional status of host cytokines and viral genes in the trachea of vaccinated and non-vaccinated chickens after challenge with the infectious laryngotracheitis virus Vagnozzi, Ariel Eduardo Riblet, Sylva Zavala, Guillermo Ecco, Roselene Afonso, Claudio L. García, Maricarmen Laryngotracheitis Cytokines Immune Response Interferons Chickens Animal Viruses Laringotraqueitis Citoquinas Respuesta Inmunológica Interferonas Pollo Virus de los Animales Infectious laryngotracheitis is a highly contagious disease of chickens responsible for significant economic losses for the poultry industry worldwide. The disease is caused by Gallid herpesvirus-1 (GaHV-1) commonly known as the infectious laryngotracheitis virus. Although characterized by their potential to regain virulence, chicken embryo origin (CEO) vaccines are the most effective vaccines against laryngotracheitis as they significantly reduce the replication of challenge virus in the trachea and conjunctiva. Knowledge on the nature of protective immunity elicited by CEO vaccines is very limited. Therefore, elucidating the origin of the immune responses elicited by CEO vaccination is relevant for development of safer control strategies. In this study the transcription levels of key host immune genes (IFN-γ, IFN-β, IL-1β, IL-6, IL-8, IL-18) and viral genes (ICP4, ICP27, UL46, UL49), as well as viral genome loads in trachea were quantified at 6 and 12 hours post-challenge of CEO vaccinated and non-vaccinated chickens. Immediately after challenge a significant increase in IFN-γ gene expression was followed by a significant reduction in viral replication. In contrast to the rapid induction of IFN-γ, expression of the pro-inflammatory cytokines (IL-1β, IL-6, IL-8) and type I IFN β was either slightly reduced or remained at basal levels. These suggest that the former cytokines may not play important roles during immediate early responses induced by ILTV challenge in either vaccinated or non-vaccinated chickens. Overall, these results suggest that the rapid expression of IFN-γ may induce pathways of antiviral responses necessary for blocking early virus replication. Instituto de Virología Fil: Vagnozzi, Ariel Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Riblet, Sylva. University of Georgia. College of Veterinary Medicine. Department of Population Health. Poultry Diagnostic and Research Center; Estados Unidos Fil: Zavala, Guillermo. Avian Health International. Flowery Branch; Estados Unidos Fil: Ecco, Roselene. Universidade Federal de Minas Gerais. Escola de Veterinária. Laboratorio de Patología; Brasil Fil: Afonso, Claudio L. Southeast Poultry Research Laboratory; Estados Unidos Fil: García, Maricarmen. University of Georgia. College of Veterinary Medicine. Department of Population Health. Poultry Diagnostic and Research Center; Estados Unidos 2019-02-13T18:22:15Z 2019-02-13T18:22:15Z 2016 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/4438 0307-9457 1465-3338 (Online) http://dx.doi.org/10.1080/03079457.2015.1126804 eng info:eu-repo/semantics/restrictedAccess application/pdf Taylor & Francis Avian pathology 45 (1) : 106–113. (2016) |
| spellingShingle | Laryngotracheitis Cytokines Immune Response Interferons Chickens Animal Viruses Laringotraqueitis Citoquinas Respuesta Inmunológica Interferonas Pollo Virus de los Animales Vagnozzi, Ariel Eduardo Riblet, Sylva Zavala, Guillermo Ecco, Roselene Afonso, Claudio L. García, Maricarmen Evaluation of the transcriptional status of host cytokines and viral genes in the trachea of vaccinated and non-vaccinated chickens after challenge with the infectious laryngotracheitis virus |
| title | Evaluation of the transcriptional status of host cytokines and viral genes in the trachea of vaccinated and non-vaccinated chickens after challenge with the infectious laryngotracheitis virus |
| title_full | Evaluation of the transcriptional status of host cytokines and viral genes in the trachea of vaccinated and non-vaccinated chickens after challenge with the infectious laryngotracheitis virus |
| title_fullStr | Evaluation of the transcriptional status of host cytokines and viral genes in the trachea of vaccinated and non-vaccinated chickens after challenge with the infectious laryngotracheitis virus |
| title_full_unstemmed | Evaluation of the transcriptional status of host cytokines and viral genes in the trachea of vaccinated and non-vaccinated chickens after challenge with the infectious laryngotracheitis virus |
| title_short | Evaluation of the transcriptional status of host cytokines and viral genes in the trachea of vaccinated and non-vaccinated chickens after challenge with the infectious laryngotracheitis virus |
| title_sort | evaluation of the transcriptional status of host cytokines and viral genes in the trachea of vaccinated and non vaccinated chickens after challenge with the infectious laryngotracheitis virus |
| topic | Laryngotracheitis Cytokines Immune Response Interferons Chickens Animal Viruses Laringotraqueitis Citoquinas Respuesta Inmunológica Interferonas Pollo Virus de los Animales |
| url | http://hdl.handle.net/20.500.12123/4438 http://dx.doi.org/10.1080/03079457.2015.1126804 |
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