Cytokine expression in the placenta of pregnant cattle after inoculation with Neospora caninum
Neospora caninum is recognized as a major cause of reproductive losses worldwide but its pathogenesis is not completely understood. Immune mediated placental pathology has been reported as being responsible for compromising pregnancy probably due to the adverse effects of exacerbated Th1 type respon...
| Autores principales: | , , , , , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2019
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| Materias: | |
| Acceso en línea: | https://www.sciencedirect.com/science/article/pii/S0165242714001676 http://hdl.handle.net/20.500.12123/4371 https://doi.org/10.1016/j.vetimm.2014.07.004 |
| _version_ | 1855483419978366976 |
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| author | Canton, German Jose Katzer, Frank Maley, Stephen W. Bartley, Paul M. Benavides-Silván, Julio Palarea-Albaladejo, Javier Pang, Yvonne Smith, Sionagh Rocchi, Mara Buxton, David Innes, Elisabeth A. Chianini, Francesca |
| author_browse | Bartley, Paul M. Benavides-Silván, Julio Buxton, David Canton, German Jose Chianini, Francesca Innes, Elisabeth A. Katzer, Frank Maley, Stephen W. Palarea-Albaladejo, Javier Pang, Yvonne Rocchi, Mara Smith, Sionagh |
| author_facet | Canton, German Jose Katzer, Frank Maley, Stephen W. Bartley, Paul M. Benavides-Silván, Julio Palarea-Albaladejo, Javier Pang, Yvonne Smith, Sionagh Rocchi, Mara Buxton, David Innes, Elisabeth A. Chianini, Francesca |
| author_sort | Canton, German Jose |
| collection | INTA Digital |
| description | Neospora caninum is recognized as a major cause of reproductive losses worldwide but its pathogenesis is not completely understood. Immune mediated placental pathology has been reported as being responsible for compromising pregnancy probably due to the adverse effects of exacerbated Th1 type response at the maternal–foetal interface. Different clinical outcomes are known to occur following experimental infections of cattle at different stages of gestation, with foetal death being the most common finding during early gestation, and the birth of live congenitally infected calves following infection later in gestation. The aim of the current study was to characterize the cytokine expression in the placenta of cattle experimentally challenged with tachyzoites of the Nc-1 strain during early, mid and late gestation. Moderate to severe infiltration of IL-12, IFN-γ and TNF-α expressing cells was observed in the placentas collected at early gestation and this infiltration was more pronounced in the samples collected from challenged dams carrying non-viable foetuses, compared with the mothers carrying viable foetuses. In contrast, the infiltration of Th1 cytokine expressing-cells was mild following N. caninum infection in mid gestation and scarce during infection in late gestation. Scarce expression of IL-4 was observed in the placentas from N. caninum-challenged and negative control animals throughout gestation. The milder Th1 immune response observed during later stages of gestation following Nc-1 infection could partially explain the less severe clinical outcome when compared to early pregnancy. |
| format | Artículo |
| id | INTA4371 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA43712019-02-01T11:56:08Z Cytokine expression in the placenta of pregnant cattle after inoculation with Neospora caninum Canton, German Jose Katzer, Frank Maley, Stephen W. Bartley, Paul M. Benavides-Silván, Julio Palarea-Albaladejo, Javier Pang, Yvonne Smith, Sionagh Rocchi, Mara Buxton, David Innes, Elisabeth A. Chianini, Francesca Ganado Bovino Neospora caninum Gestación Enfermedades de los Animales Citoquinas Placenta Cattle Pregnancy Animal Diseases Cytokines Neospora caninum is recognized as a major cause of reproductive losses worldwide but its pathogenesis is not completely understood. Immune mediated placental pathology has been reported as being responsible for compromising pregnancy probably due to the adverse effects of exacerbated Th1 type response at the maternal–foetal interface. Different clinical outcomes are known to occur following experimental infections of cattle at different stages of gestation, with foetal death being the most common finding during early gestation, and the birth of live congenitally infected calves following infection later in gestation. The aim of the current study was to characterize the cytokine expression in the placenta of cattle experimentally challenged with tachyzoites of the Nc-1 strain during early, mid and late gestation. Moderate to severe infiltration of IL-12, IFN-γ and TNF-α expressing cells was observed in the placentas collected at early gestation and this infiltration was more pronounced in the samples collected from challenged dams carrying non-viable foetuses, compared with the mothers carrying viable foetuses. In contrast, the infiltration of Th1 cytokine expressing-cells was mild following N. caninum infection in mid gestation and scarce during infection in late gestation. Scarce expression of IL-4 was observed in the placentas from N. caninum-challenged and negative control animals throughout gestation. The milder Th1 immune response observed during later stages of gestation following Nc-1 infection could partially explain the less severe clinical outcome when compared to early pregnancy. EEA Balcarce Fil: Canton, German Jose. Moredun Research Institute; Reino Unido. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Katzer, Frank. Moredun Research Institute; Reino Unido Fil: Maley, Stephen W. Moredun Research Institute; Reino Unido Fil: Bartley, Paul M. Moredun Research Institute; Reino Unido Fil: Benavides-Silván, Julio. Moredun Research Institute; Reino Unido. Consejo Superior de Investigaciones Científicas. Instituto de Ganadería de Montaña; España Fil: Palarea-Albaladejo, Javier. Biomathematics & Statistics Scotland; Reino Unido Fil: Pang, Yvonne. Moredun Research Institute; Reino Unido Fil: Smith, Sionagh. University of Edinburgh. Royal (Dick) School of Veterinary Studies and Roslin Institute; Reino Unido Fil: Rocchi, Mara. Moredun Research Institute; Reino Unido Fil: Buxton, David. Moredun Research Institute; Reino Unido Fil: Innes, Elisabeth A. Moredun Research Institute; Reino Unido Fil: Chianini, Francesca. Moredun Research Institute; Reino Unido 2019-02-01T11:54:30Z 2019-02-01T11:54:30Z 2014-09-15 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.sciencedirect.com/science/article/pii/S0165242714001676 http://hdl.handle.net/20.500.12123/4371 0165-2427 1873-2534 https://doi.org/10.1016/j.vetimm.2014.07.004 eng info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Veterinary Immunology and Immunopathology 161 (1–2) : 77-89 (September 2014) |
| spellingShingle | Ganado Bovino Neospora caninum Gestación Enfermedades de los Animales Citoquinas Placenta Cattle Pregnancy Animal Diseases Cytokines Canton, German Jose Katzer, Frank Maley, Stephen W. Bartley, Paul M. Benavides-Silván, Julio Palarea-Albaladejo, Javier Pang, Yvonne Smith, Sionagh Rocchi, Mara Buxton, David Innes, Elisabeth A. Chianini, Francesca Cytokine expression in the placenta of pregnant cattle after inoculation with Neospora caninum |
| title | Cytokine expression in the placenta of pregnant cattle after inoculation with Neospora caninum |
| title_full | Cytokine expression in the placenta of pregnant cattle after inoculation with Neospora caninum |
| title_fullStr | Cytokine expression in the placenta of pregnant cattle after inoculation with Neospora caninum |
| title_full_unstemmed | Cytokine expression in the placenta of pregnant cattle after inoculation with Neospora caninum |
| title_short | Cytokine expression in the placenta of pregnant cattle after inoculation with Neospora caninum |
| title_sort | cytokine expression in the placenta of pregnant cattle after inoculation with neospora caninum |
| topic | Ganado Bovino Neospora caninum Gestación Enfermedades de los Animales Citoquinas Placenta Cattle Pregnancy Animal Diseases Cytokines |
| url | https://www.sciencedirect.com/science/article/pii/S0165242714001676 http://hdl.handle.net/20.500.12123/4371 https://doi.org/10.1016/j.vetimm.2014.07.004 |
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