High rate of transplacental infection and transmission of Neospora caninum following experimental challenge of cattle at day 210 of gestation
In order to investigate the pathogenesis of neosporosis following a primary infection in late pregnancy, cattle were subcutaneously challenged with 5 × 108 Neospora caninum (NC1 isolate) tachyzoites at day 210 of gestation and serial necropsies were then carried out at 14, 28, 42 and 56 days post-in...
| Autores principales: | , , , , , , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
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BMC
2019
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| Materias: | |
| Acceso en línea: | https://veterinaryresearch.biomedcentral.com/articles/10.1186/1297-9716-43-83 http://hdl.handle.net/20.500.12123/4366 https://doi.org/10.1186/1297-9716-43-83 |
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| author | Benavides-Silván, Julio Katzer, Frank Maley, Stephen W. Bartley, Paul M. Canton, German Jose Palarea-Albaladejo, Javier Purslow, Caroline A. Pang, Yvonne Rocchi, Mara Chianini, Francesca Buxton, David Innes, Elisabeth A. |
| author_browse | Bartley, Paul M. Benavides-Silván, Julio Buxton, David Canton, German Jose Chianini, Francesca Innes, Elisabeth A. Katzer, Frank Maley, Stephen W. Palarea-Albaladejo, Javier Pang, Yvonne Purslow, Caroline A. Rocchi, Mara |
| author_facet | Benavides-Silván, Julio Katzer, Frank Maley, Stephen W. Bartley, Paul M. Canton, German Jose Palarea-Albaladejo, Javier Purslow, Caroline A. Pang, Yvonne Rocchi, Mara Chianini, Francesca Buxton, David Innes, Elisabeth A. |
| author_sort | Benavides-Silván, Julio |
| collection | INTA Digital |
| description | In order to investigate the pathogenesis of neosporosis following a primary infection in late pregnancy, cattle were subcutaneously challenged with 5 × 108 Neospora caninum (NC1 isolate) tachyzoites at day 210 of gestation and serial necropsies were then carried out at 14, 28, 42 and 56 days post-infection (dpi). No abortions occurred and all the foetuses were viable at the time of euthanasia. There was a high rate of vertical transmission, as parasites were detected by immunohistochemical labelling and PCR in all the foetuses from 28 dpi. Focal necrotic lesions were observed in the placentomes of the placenta from 28 dpi and showed resolution during later time points, denoted by infiltration of inflammatory cells at 42 dpi and fibrosis at 56 dpi. Foetuses at 28 and 42 dpi showed scarce and isolated lesions which are unlikely to represent a threat to foetal viability. No lesions were observed in the foetuses at 14 or 56 dpi suggesting control of the infection and resolution of the lesions by maternal and foetal immune responses. Once infection was established, it could not be cleared from the host and vertical transmission of the parasite occurred in all infected hosts. Parasite was detected in the placenta at 28 dpi, while in previous experimental infections of cattle at day 70 and 140 of gestation using the same challenge model, it was already present at day 14 post infection. This suggests that a change in the maternal immune response plays a crucial role in limiting the initial infection during the last term of pregnancy. |
| format | info:ar-repo/semantics/artículo |
| id | INTA4366 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | BMC |
| publisherStr | BMC |
| record_format | dspace |
| spelling | INTA43662019-01-31T13:19:57Z High rate of transplacental infection and transmission of Neospora caninum following experimental challenge of cattle at day 210 of gestation Benavides-Silván, Julio Katzer, Frank Maley, Stephen W. Bartley, Paul M. Canton, German Jose Palarea-Albaladejo, Javier Purslow, Caroline A. Pang, Yvonne Rocchi, Mara Chianini, Francesca Buxton, David Innes, Elisabeth A. Ganado Bovino Enfermedades de los Animales Neospora caninum Gestación Inmunidad Maternal Respuesta Inmunológica Cattle Animal Diseases Pregnancy Maternal Immunity Immune Response In order to investigate the pathogenesis of neosporosis following a primary infection in late pregnancy, cattle were subcutaneously challenged with 5 × 108 Neospora caninum (NC1 isolate) tachyzoites at day 210 of gestation and serial necropsies were then carried out at 14, 28, 42 and 56 days post-infection (dpi). No abortions occurred and all the foetuses were viable at the time of euthanasia. There was a high rate of vertical transmission, as parasites were detected by immunohistochemical labelling and PCR in all the foetuses from 28 dpi. Focal necrotic lesions were observed in the placentomes of the placenta from 28 dpi and showed resolution during later time points, denoted by infiltration of inflammatory cells at 42 dpi and fibrosis at 56 dpi. Foetuses at 28 and 42 dpi showed scarce and isolated lesions which are unlikely to represent a threat to foetal viability. No lesions were observed in the foetuses at 14 or 56 dpi suggesting control of the infection and resolution of the lesions by maternal and foetal immune responses. Once infection was established, it could not be cleared from the host and vertical transmission of the parasite occurred in all infected hosts. Parasite was detected in the placenta at 28 dpi, while in previous experimental infections of cattle at day 70 and 140 of gestation using the same challenge model, it was already present at day 14 post infection. This suggests that a change in the maternal immune response plays a crucial role in limiting the initial infection during the last term of pregnancy. EEA Balcarce Fil: Benavides-Silván, Julio. Moredun Research Institute; Reino Unido. Consejo Superior de Investigaciones Científicas. Instituto de Ganadería de Montaña; España Fil: Katzer, Frank. Moredun Research Institute; Reino Unido Fil: Maley, Stephen W. Moredun Research Institute; Reino Unido Fil: Bartley, Paul M. Moredun Research Institute; Reino Unido Fil: Canton, German Jose. Moredun Research Institute; Reino Unido. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Palarea-Albaladejo, Javier. Biomathematics & Statistics Scotland; Reino Unido Fil: Purslow, Caroline A. Moredun Research Institute; Reino Unido Fil: Pang, Yvonne. Moredun Research Institute; Reino Unido Fil: Rocchi, Mara. Moredun Research Institute; Reino Unido Fil: Chianini, Francesca. Moredun Research Institute; Reino Unido Fil: Buxton, David. Moredun Research Institute; Reino Unido Fil: Innes, Elisabeth A. Moredun Research Institute; Reino Unido 2019-01-31T13:16:29Z 2019-01-31T13:16:29Z 2012-12 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://veterinaryresearch.biomedcentral.com/articles/10.1186/1297-9716-43-83 http://hdl.handle.net/20.500.12123/4366 0928-4249 1297-9716 https://doi.org/10.1186/1297-9716-43-83 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf BMC Veterinary Research 43 : 83 (2012) |
| spellingShingle | Ganado Bovino Enfermedades de los Animales Neospora caninum Gestación Inmunidad Maternal Respuesta Inmunológica Cattle Animal Diseases Pregnancy Maternal Immunity Immune Response Benavides-Silván, Julio Katzer, Frank Maley, Stephen W. Bartley, Paul M. Canton, German Jose Palarea-Albaladejo, Javier Purslow, Caroline A. Pang, Yvonne Rocchi, Mara Chianini, Francesca Buxton, David Innes, Elisabeth A. High rate of transplacental infection and transmission of Neospora caninum following experimental challenge of cattle at day 210 of gestation |
| title | High rate of transplacental infection and transmission of Neospora caninum following experimental challenge of cattle at day 210 of gestation |
| title_full | High rate of transplacental infection and transmission of Neospora caninum following experimental challenge of cattle at day 210 of gestation |
| title_fullStr | High rate of transplacental infection and transmission of Neospora caninum following experimental challenge of cattle at day 210 of gestation |
| title_full_unstemmed | High rate of transplacental infection and transmission of Neospora caninum following experimental challenge of cattle at day 210 of gestation |
| title_short | High rate of transplacental infection and transmission of Neospora caninum following experimental challenge of cattle at day 210 of gestation |
| title_sort | high rate of transplacental infection and transmission of neospora caninum following experimental challenge of cattle at day 210 of gestation |
| topic | Ganado Bovino Enfermedades de los Animales Neospora caninum Gestación Inmunidad Maternal Respuesta Inmunológica Cattle Animal Diseases Pregnancy Maternal Immunity Immune Response |
| url | https://veterinaryresearch.biomedcentral.com/articles/10.1186/1297-9716-43-83 http://hdl.handle.net/20.500.12123/4366 https://doi.org/10.1186/1297-9716-43-83 |
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