Standard methods for fungal brood disease research = Métodos estándar para la investigación de enfermedades fúngicas de la cría
Chalkbrood and stonebrood are two fungal diseases associated with honey bee brood. Chalkbrood, caused by Ascosphaera apis, is a common and widespread disease that can result in severe reduction of emerging worker bees and thus overall colony productivity. Stonebrood is caused by Aspergillus spp. tha...
| Autores principales: | , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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Taylor & Francis
2019
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| Acceso en línea: | https://www.tandfonline.com/doi/abs/10.3896/IBRA.1.52.1.13 http://hdl.handle.net/20.500.12123/4363 https://doi.org/10.3896/IBRA.1.52.1.13 |
| _version_ | 1855483418288062464 |
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| author | Jensen, Annette Bruun Aronstein, Kathrine Flores, José Manuel Vojvodic, Svjetlana Palacio, María Alejandra Spivak, Marla |
| author_browse | Aronstein, Kathrine Flores, José Manuel Jensen, Annette Bruun Palacio, María Alejandra Spivak, Marla Vojvodic, Svjetlana |
| author_facet | Jensen, Annette Bruun Aronstein, Kathrine Flores, José Manuel Vojvodic, Svjetlana Palacio, María Alejandra Spivak, Marla |
| author_sort | Jensen, Annette Bruun |
| collection | INTA Digital |
| description | Chalkbrood and stonebrood are two fungal diseases associated with honey bee brood. Chalkbrood, caused by Ascosphaera apis, is a common and widespread disease that can result in severe reduction of emerging worker bees and thus overall colony productivity. Stonebrood is caused by Aspergillus spp. that are rarely observed, so the impact on colony health is not very well understood. A major concern with the presence of Aspergillus in honey bees is the production of airborne conidia, which can lead to allergic bronchopulmonary aspergillosis, pulmonary aspergilloma, or even invasive aspergillosis in lung tissues upon inhalation by humans. In the current chapter we describe the honey bee disease symptoms of these fungal pathogens. In addition, we provide research methodologies and protocols for isolating and culturing, in vivo and in vitro assays that are commonly used to study these host pathogen interactions. We give guidelines on the preferred methods used in current research and the application of molecular techniques. We have added photographs, drawings and illustrations to assist bee-extension personnel and bee scientists in the control of these two diseases. |
| format | Artículo |
| id | INTA4363 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Taylor & Francis |
| publisherStr | Taylor & Francis |
| record_format | dspace |
| spelling | INTA43632019-01-31T12:34:26Z Standard methods for fungal brood disease research = Métodos estándar para la investigación de enfermedades fúngicas de la cría Jensen, Annette Bruun Aronstein, Kathrine Flores, José Manuel Vojvodic, Svjetlana Palacio, María Alejandra Spivak, Marla Enfermedades de los Animales Incubación de Abejas Enfermedades Fungosas Chalk Brood Ascosphaera apis Abeja Melífera Aspergillus Animal Diseases Bee Broods Fungal Diseases Honey Bees Chalkbrood and stonebrood are two fungal diseases associated with honey bee brood. Chalkbrood, caused by Ascosphaera apis, is a common and widespread disease that can result in severe reduction of emerging worker bees and thus overall colony productivity. Stonebrood is caused by Aspergillus spp. that are rarely observed, so the impact on colony health is not very well understood. A major concern with the presence of Aspergillus in honey bees is the production of airborne conidia, which can lead to allergic bronchopulmonary aspergillosis, pulmonary aspergilloma, or even invasive aspergillosis in lung tissues upon inhalation by humans. In the current chapter we describe the honey bee disease symptoms of these fungal pathogens. In addition, we provide research methodologies and protocols for isolating and culturing, in vivo and in vitro assays that are commonly used to study these host pathogen interactions. We give guidelines on the preferred methods used in current research and the application of molecular techniques. We have added photographs, drawings and illustrations to assist bee-extension personnel and bee scientists in the control of these two diseases. La ascosferiosis (o cría yesificada) y cría de piedra son dos enfermedades fúngicas asociadas con la cría de la abeja melífera. La ascosferiosis, causada por el hongo Ascosphaera apis, es una enfermedad comú;n de amplia distribución que puede resultar en una severa disminución en el número de obreras emergentes y por lo tanto afecta la productividad de la colonia. La cría de piedra es causada por Aspergillus spp. los cuales son raramente observados y su impacto en la salud de la colonia no está esclarecido. El mayor problema con la presencia de Aspergillus en abejas es la producción de conidios aéreos, que pueden llevar a aspergillosis broncopulmonares alérgicas, aspergilomas pulmonares o aun aspergilosis invasivas en tejidos pulmonares en humanos luego de inhalarlos. En este capítulo describimos los síntomas de las enfermedades producidas por estos patógenos fú;ngicos. Además proveemos metodologías de investigación y protocolos para su aislamiento y cultivo, ensayos in vivo e in vitro que son comú;nmente usados para estudiar las interacciones de estos patógenos con su hospedero. Damos una guía sobre los métodos preferidos utilizados en la investigación actual y la aplicación de técnicas de moleculares. Hemos añadido fotografías, dibujos e ilustraciones para ayudar al personal. EEA Balcarce Fil: Jensen, Annette Bruun. University of Copenhagen. Department of Plant and Environmental Sciences; Dinamarca Fil: Aronstein, Kathrine. USDA-ARS. Honey Bee Research Unit; Estados Unidos Fil: Flores, José Manuel. University of Córdoba. Department of Zoology; España Fil: Vojvodic, Svjetlana. University of Arizona. Center for Insect Science; Estados Unidos Fil: Palacio, María Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina Fil: Spivak, Marla. University of Minnesota. Department of Entomology; Estados Unidos 2019-01-31T12:32:28Z 2019-01-31T12:32:28Z 2013 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.tandfonline.com/doi/abs/10.3896/IBRA.1.52.1.13 http://hdl.handle.net/20.500.12123/4363 0021-8839 2078-6913 https://doi.org/10.3896/IBRA.1.52.1.13 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Taylor & Francis Journal of Apicultural Research 52 (1) : 1-20 (2013) |
| spellingShingle | Enfermedades de los Animales Incubación de Abejas Enfermedades Fungosas Chalk Brood Ascosphaera apis Abeja Melífera Aspergillus Animal Diseases Bee Broods Fungal Diseases Honey Bees Jensen, Annette Bruun Aronstein, Kathrine Flores, José Manuel Vojvodic, Svjetlana Palacio, María Alejandra Spivak, Marla Standard methods for fungal brood disease research = Métodos estándar para la investigación de enfermedades fúngicas de la cría |
| title | Standard methods for fungal brood disease research = Métodos estándar para la investigación de enfermedades fúngicas de la cría |
| title_full | Standard methods for fungal brood disease research = Métodos estándar para la investigación de enfermedades fúngicas de la cría |
| title_fullStr | Standard methods for fungal brood disease research = Métodos estándar para la investigación de enfermedades fúngicas de la cría |
| title_full_unstemmed | Standard methods for fungal brood disease research = Métodos estándar para la investigación de enfermedades fúngicas de la cría |
| title_short | Standard methods for fungal brood disease research = Métodos estándar para la investigación de enfermedades fúngicas de la cría |
| title_sort | standard methods for fungal brood disease research metodos estandar para la investigacion de enfermedades fungicas de la cria |
| topic | Enfermedades de los Animales Incubación de Abejas Enfermedades Fungosas Chalk Brood Ascosphaera apis Abeja Melífera Aspergillus Animal Diseases Bee Broods Fungal Diseases Honey Bees |
| url | https://www.tandfonline.com/doi/abs/10.3896/IBRA.1.52.1.13 http://hdl.handle.net/20.500.12123/4363 https://doi.org/10.3896/IBRA.1.52.1.13 |
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