A Neospora caninum vaccine using recombinant proteins fails to prevent foetal infection in pregnant cattle after experimental intravenous challenge
The aim of the present study was to evaluate the immunogenicity and protective efficacy of rNcSAG1, rNcHSP20 and rNcGRA7 recombinant proteins formulated with immune stimulating complexes (ISCOMs) in pregnant heifers against vertical transmission of Neospora caninum. Twelve pregnant heifers were divi...
| Autores principales: | , , , , , , , , , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2019
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| Materias: | |
| Acceso en línea: | https://www.sciencedirect.com/science/article/pii/S016524271400258X http://hdl.handle.net/20.500.12123/4360 https://doi.org/10.1016/j.vetimm.2014.11.001 |
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| author | Hecker, Yanina Paola Cóceres, Verónica Mabel Wilkowsky, Silvina Elizabeth Jaramillo Ortiz, Jose Manuel Morrell, Eleonora Lidia Verna, Andrea Elizabeth Ganuza, Agustina Cano, Dora Beatriz Lischinsky, Lilian Haydee Ángel, Sergio Oscar Zamorano, Patricia Ines Odeon, Anselmo Carlos Leunda, Maria Rosa Campero, Carlos Manuel Morein, Bror Moore, Prando Dadin |
| author_browse | Campero, Carlos Manuel Cano, Dora Beatriz Cóceres, Verónica Mabel Ganuza, Agustina Hecker, Yanina Paola Jaramillo Ortiz, Jose Manuel Leunda, Maria Rosa Lischinsky, Lilian Haydee Moore, Prando Dadin Morein, Bror Morrell, Eleonora Lidia Odeon, Anselmo Carlos Verna, Andrea Elizabeth Wilkowsky, Silvina Elizabeth Zamorano, Patricia Ines Ángel, Sergio Oscar |
| author_facet | Hecker, Yanina Paola Cóceres, Verónica Mabel Wilkowsky, Silvina Elizabeth Jaramillo Ortiz, Jose Manuel Morrell, Eleonora Lidia Verna, Andrea Elizabeth Ganuza, Agustina Cano, Dora Beatriz Lischinsky, Lilian Haydee Ángel, Sergio Oscar Zamorano, Patricia Ines Odeon, Anselmo Carlos Leunda, Maria Rosa Campero, Carlos Manuel Morein, Bror Moore, Prando Dadin |
| author_sort | Hecker, Yanina Paola |
| collection | INTA Digital |
| description | The aim of the present study was to evaluate the immunogenicity and protective efficacy of rNcSAG1, rNcHSP20 and rNcGRA7 recombinant proteins formulated with immune stimulating complexes (ISCOMs) in pregnant heifers against vertical transmission of Neospora caninum. Twelve pregnant heifers were divided into 3 groups of 4 heifers each, receiving different formulations before mating. Immunogens were administered twice subcutaneously: group A animals were inoculated with three recombinant proteins (rNcSAG1, rNcHSP20, rNcGRA7) formulated with ISCOMs; group B animals received ISCOM-MATRIX (without antigen) and group C received sterile phosphate-buffered saline (PBS) only. The recombinant proteins were expressed in Escherichia coli and purified nickel resin. All groups were intravenously challenged with the NC-1 strain of N. caninum at Day 70 of gestation and dams slaughtered at week 17 of the experiment. Heifers from group A developed specific antibodies against rNcSAG1, rNcHSP20 and rNcGRA7 prior to the challenge. Following immunization, an statistically significant increase of antibodies against rNcSAG1 and rNcHSP20 in all animals of group A was detected compared to animals in groups B and C at weeks 5, 13 and 16 (P < 0.001). Levels of antibodies against rNcGRA7 were statistical higher in group A animals when compared with groups B and C at weeks 5 and 16 (P > 0.001). There were no differences in IFN-γ production among the experimental groups at any time point (P > 0.05). Transplacental transmission was determined in all foetuses of groups A, B and C by Western blot, immunohistochemistry and nested PCR. This work showed that rNcSAG1, rNcHSP20 and rNcGRA7 proteins while immunogenic in cattle failed to prevent the foetal infection in pregnant cattle challenged at Day 70 of gestation. |
| format | Artículo |
| id | INTA4360 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA43602019-06-11T14:30:02Z A Neospora caninum vaccine using recombinant proteins fails to prevent foetal infection in pregnant cattle after experimental intravenous challenge Hecker, Yanina Paola Cóceres, Verónica Mabel Wilkowsky, Silvina Elizabeth Jaramillo Ortiz, Jose Manuel Morrell, Eleonora Lidia Verna, Andrea Elizabeth Ganuza, Agustina Cano, Dora Beatriz Lischinsky, Lilian Haydee Ángel, Sergio Oscar Zamorano, Patricia Ines Odeon, Anselmo Carlos Leunda, Maria Rosa Campero, Carlos Manuel Morein, Bror Moore, Prando Dadin Ganado Bovino Gestación Vacuna Neospora caninum Anticuerpos Inmunidad Maternal Cattle Pregnancy Vaccines Antibodies Maternal Immunity The aim of the present study was to evaluate the immunogenicity and protective efficacy of rNcSAG1, rNcHSP20 and rNcGRA7 recombinant proteins formulated with immune stimulating complexes (ISCOMs) in pregnant heifers against vertical transmission of Neospora caninum. Twelve pregnant heifers were divided into 3 groups of 4 heifers each, receiving different formulations before mating. Immunogens were administered twice subcutaneously: group A animals were inoculated with three recombinant proteins (rNcSAG1, rNcHSP20, rNcGRA7) formulated with ISCOMs; group B animals received ISCOM-MATRIX (without antigen) and group C received sterile phosphate-buffered saline (PBS) only. The recombinant proteins were expressed in Escherichia coli and purified nickel resin. All groups were intravenously challenged with the NC-1 strain of N. caninum at Day 70 of gestation and dams slaughtered at week 17 of the experiment. Heifers from group A developed specific antibodies against rNcSAG1, rNcHSP20 and rNcGRA7 prior to the challenge. Following immunization, an statistically significant increase of antibodies against rNcSAG1 and rNcHSP20 in all animals of group A was detected compared to animals in groups B and C at weeks 5, 13 and 16 (P < 0.001). Levels of antibodies against rNcGRA7 were statistical higher in group A animals when compared with groups B and C at weeks 5 and 16 (P > 0.001). There were no differences in IFN-γ production among the experimental groups at any time point (P > 0.05). Transplacental transmission was determined in all foetuses of groups A, B and C by Western blot, immunohistochemistry and nested PCR. This work showed that rNcSAG1, rNcHSP20 and rNcGRA7 proteins while immunogenic in cattle failed to prevent the foetal infection in pregnant cattle challenged at Day 70 of gestation. EEA Balcarce Fil: Hecker, Yanina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Cóceres, Verónica Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas. Laboratorio de Parasitología Molecular; Argentina Fil: Wilkowsky, Silvina Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Jaramillo Ortiz, Jose Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Morrell, Eleonora Lidia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Verna, Andrea Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Ganuza, Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas. Laboratorio de Parasitología Molecular; Argentina Fil: Cano, Dora Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Lischinsky, Lilian Haydee. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Ángel, Sergio Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas. Laboratorio de Parasitología Molecular; Argentina Fil: Zamorano, Patricia Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Odeon, Anselmo Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Leunda, Maria Rosa. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Campero, Carlos Manuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Morein, Bror. Uppsala University. Department of Medical Sciences. Infectious Diseases; Suecia Fil: Moore, Prando Dadin. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina 2019-01-30T14:35:07Z 2019-01-30T14:35:07Z 2014-12-15 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.sciencedirect.com/science/article/pii/S016524271400258X http://hdl.handle.net/20.500.12123/4360 0165-2427 https://doi.org/10.1016/j.vetimm.2014.11.001 eng info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Veterinary Immunology and Immunopathology 162 (3–4) : 142-153 (December 2014) |
| spellingShingle | Ganado Bovino Gestación Vacuna Neospora caninum Anticuerpos Inmunidad Maternal Cattle Pregnancy Vaccines Antibodies Maternal Immunity Hecker, Yanina Paola Cóceres, Verónica Mabel Wilkowsky, Silvina Elizabeth Jaramillo Ortiz, Jose Manuel Morrell, Eleonora Lidia Verna, Andrea Elizabeth Ganuza, Agustina Cano, Dora Beatriz Lischinsky, Lilian Haydee Ángel, Sergio Oscar Zamorano, Patricia Ines Odeon, Anselmo Carlos Leunda, Maria Rosa Campero, Carlos Manuel Morein, Bror Moore, Prando Dadin A Neospora caninum vaccine using recombinant proteins fails to prevent foetal infection in pregnant cattle after experimental intravenous challenge |
| title | A Neospora caninum vaccine using recombinant proteins fails to prevent foetal infection in pregnant cattle after experimental intravenous challenge |
| title_full | A Neospora caninum vaccine using recombinant proteins fails to prevent foetal infection in pregnant cattle after experimental intravenous challenge |
| title_fullStr | A Neospora caninum vaccine using recombinant proteins fails to prevent foetal infection in pregnant cattle after experimental intravenous challenge |
| title_full_unstemmed | A Neospora caninum vaccine using recombinant proteins fails to prevent foetal infection in pregnant cattle after experimental intravenous challenge |
| title_short | A Neospora caninum vaccine using recombinant proteins fails to prevent foetal infection in pregnant cattle after experimental intravenous challenge |
| title_sort | neospora caninum vaccine using recombinant proteins fails to prevent foetal infection in pregnant cattle after experimental intravenous challenge |
| topic | Ganado Bovino Gestación Vacuna Neospora caninum Anticuerpos Inmunidad Maternal Cattle Pregnancy Vaccines Antibodies Maternal Immunity |
| url | https://www.sciencedirect.com/science/article/pii/S016524271400258X http://hdl.handle.net/20.500.12123/4360 https://doi.org/10.1016/j.vetimm.2014.11.001 |
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