Sunflower germin-like protein HaGLP1 promotes ROS accumulation and enhances protection against fungal pathogens in transgenic Arabidopsis thaliana
Germin-like proteins (GLPs) are a large, diverse and ubiquitous family of plant glycoproteins belonging to the Cupin super family. These proteins have been widely studied because of their diverse roles in important plant processes, including defence. The novel sunflower gene HaGLP1 encodes the first...
| Autores principales: | , , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Springer
2019
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| Materias: | |
| Acceso en línea: | https://link.springer.com/article/10.1007/s00299-015-1819-4 http://hdl.handle.net/20.500.12123/4358 https://doi.org/10.1007/s00299-015-1819-4 |
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| author | Beracochea, Valeria Cecilia Almasia, Natalia Ines Peluffo, Lucila Nahirñak, Vanesa Hopp, Horacio Esteban Paniego, Norma Beatriz Heinz, Ruth Amelia Vazquez Rovere, Cecilia Lia, Veronica Viviana |
| author_browse | Almasia, Natalia Ines Beracochea, Valeria Cecilia Heinz, Ruth Amelia Hopp, Horacio Esteban Lia, Veronica Viviana Nahirñak, Vanesa Paniego, Norma Beatriz Peluffo, Lucila Vazquez Rovere, Cecilia |
| author_facet | Beracochea, Valeria Cecilia Almasia, Natalia Ines Peluffo, Lucila Nahirñak, Vanesa Hopp, Horacio Esteban Paniego, Norma Beatriz Heinz, Ruth Amelia Vazquez Rovere, Cecilia Lia, Veronica Viviana |
| author_sort | Beracochea, Valeria Cecilia |
| collection | INTA Digital |
| description | Germin-like proteins (GLPs) are a large, diverse and ubiquitous family of plant glycoproteins belonging to the Cupin super family. These proteins have been widely studied because of their diverse roles in important plant processes, including defence. The novel sunflower gene HaGLP1 encodes the first germin-like protein characterized from the family Asteraceae. To analyse whether constitutive in vivo expression of the HaGLP1 gene may lead to disease tolerance, we developed transgenic Arabidopsis plants that were molecularly characterized and biologically assessed after inoculation with Sclerotinia sclerotiorum or Rhizoctonia solani. HaGLP1 expression in Arabidopsis plants conferred tolerance to S. sclerotiorum at the first stages of disease and interfered with R. solani infection, thus giving rise to significant protection against the latter. Furthermore, HaGLP1 expression in Arabidopsis plants elevated endogenous ROS levels. HaGLP1-induced tolerance does not appear to be related to a constitutive induction of the plant defence or the ROS-related genes examined here. In conclusion, our data suggest that HaGLP1 is an interesting candidate for the engineering of plants with increased fungal tolerance and that this gene could also be useful for the selection of naturally overexpressing sunflower genotypes for conventional breeding purposes. |
| format | Artículo |
| id | INTA4358 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA43582019-01-30T13:33:18Z Sunflower germin-like protein HaGLP1 promotes ROS accumulation and enhances protection against fungal pathogens in transgenic Arabidopsis thaliana Beracochea, Valeria Cecilia Almasia, Natalia Ines Peluffo, Lucila Nahirñak, Vanesa Hopp, Horacio Esteban Paniego, Norma Beatriz Heinz, Ruth Amelia Vazquez Rovere, Cecilia Lia, Veronica Viviana Helianthus Annuus Genes Genética Control de Enfermedades Enfermedades Fungosas Arabidopsis Thaliana Rhizoctonia solani Sclerotinia sclerotiorum Genetics Disease Control Fungal Diseases Girasol Germin-like proteins (GLPs) are a large, diverse and ubiquitous family of plant glycoproteins belonging to the Cupin super family. These proteins have been widely studied because of their diverse roles in important plant processes, including defence. The novel sunflower gene HaGLP1 encodes the first germin-like protein characterized from the family Asteraceae. To analyse whether constitutive in vivo expression of the HaGLP1 gene may lead to disease tolerance, we developed transgenic Arabidopsis plants that were molecularly characterized and biologically assessed after inoculation with Sclerotinia sclerotiorum or Rhizoctonia solani. HaGLP1 expression in Arabidopsis plants conferred tolerance to S. sclerotiorum at the first stages of disease and interfered with R. solani infection, thus giving rise to significant protection against the latter. Furthermore, HaGLP1 expression in Arabidopsis plants elevated endogenous ROS levels. HaGLP1-induced tolerance does not appear to be related to a constitutive induction of the plant defence or the ROS-related genes examined here. In conclusion, our data suggest that HaGLP1 is an interesting candidate for the engineering of plants with increased fungal tolerance and that this gene could also be useful for the selection of naturally overexpressing sunflower genotypes for conventional breeding purposes. Instituto de Biotecnología Fil: Beracochea, Valeria Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Almasia, Natalia Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Peluffo, Lucila. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Nahirñak, Vanesa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Hopp, Horacio Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina Fil: Paniego, Norma Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Heinz, Ruth Amelia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina Fil: Vazquez Rovere, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Lia, Veronica Viviana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina 2019-01-30T13:31:39Z 2019-01-30T13:31:39Z 2015-10 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://link.springer.com/article/10.1007/s00299-015-1819-4 http://hdl.handle.net/20.500.12123/4358 0721-7714 1432-203X https://doi.org/10.1007/s00299-015-1819-4 eng info:eu-repo/semantics/restrictedAccess application/pdf Springer Plant Cell Reports 34 (10) : 1717–1733 (October 2015) |
| spellingShingle | Helianthus Annuus Genes Genética Control de Enfermedades Enfermedades Fungosas Arabidopsis Thaliana Rhizoctonia solani Sclerotinia sclerotiorum Genetics Disease Control Fungal Diseases Girasol Beracochea, Valeria Cecilia Almasia, Natalia Ines Peluffo, Lucila Nahirñak, Vanesa Hopp, Horacio Esteban Paniego, Norma Beatriz Heinz, Ruth Amelia Vazquez Rovere, Cecilia Lia, Veronica Viviana Sunflower germin-like protein HaGLP1 promotes ROS accumulation and enhances protection against fungal pathogens in transgenic Arabidopsis thaliana |
| title | Sunflower germin-like protein HaGLP1 promotes ROS accumulation and enhances protection against fungal pathogens in transgenic Arabidopsis thaliana |
| title_full | Sunflower germin-like protein HaGLP1 promotes ROS accumulation and enhances protection against fungal pathogens in transgenic Arabidopsis thaliana |
| title_fullStr | Sunflower germin-like protein HaGLP1 promotes ROS accumulation and enhances protection against fungal pathogens in transgenic Arabidopsis thaliana |
| title_full_unstemmed | Sunflower germin-like protein HaGLP1 promotes ROS accumulation and enhances protection against fungal pathogens in transgenic Arabidopsis thaliana |
| title_short | Sunflower germin-like protein HaGLP1 promotes ROS accumulation and enhances protection against fungal pathogens in transgenic Arabidopsis thaliana |
| title_sort | sunflower germin like protein haglp1 promotes ros accumulation and enhances protection against fungal pathogens in transgenic arabidopsis thaliana |
| topic | Helianthus Annuus Genes Genética Control de Enfermedades Enfermedades Fungosas Arabidopsis Thaliana Rhizoctonia solani Sclerotinia sclerotiorum Genetics Disease Control Fungal Diseases Girasol |
| url | https://link.springer.com/article/10.1007/s00299-015-1819-4 http://hdl.handle.net/20.500.12123/4358 https://doi.org/10.1007/s00299-015-1819-4 |
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