Double-layered rotavirus-like particles are efficient carriers to elicit strong CTL responses to delivered heterologous antigens
The first 92 amino acids of VP2 of rotavirus are dispensable for VLP assembly and can be replaced by heterologous reporter proteins without affecting either self-assembly of chimeric VP2 or the interaction with VP6 to render chimeric VP2/6 VLPs. In this study, we constructed recombinant baculoviruse...
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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Elsevier
2019
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| Online Access: | https://www.sciencedirect.com/science/article/pii/S1359511314004000 http://hdl.handle.net/20.500.12123/4357 https://doi.org/10.1016/j.procbio.2014.07.014 |
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| author | Molinari, Maria Paula Peralta, Andrea Veronica Maletto, Belkys Angélica Pistoresi Palencia, Maria Cristina Crespo, Maria Ines Moron, Victor Gabriel Taboga, Oscar Alberto |
| author_browse | Crespo, Maria Ines Maletto, Belkys Angélica Molinari, Maria Paula Moron, Victor Gabriel Peralta, Andrea Veronica Pistoresi Palencia, Maria Cristina Taboga, Oscar Alberto |
| author_facet | Molinari, Maria Paula Peralta, Andrea Veronica Maletto, Belkys Angélica Pistoresi Palencia, Maria Cristina Crespo, Maria Ines Moron, Victor Gabriel Taboga, Oscar Alberto |
| author_sort | Molinari, Maria Paula |
| collection | INTA Digital |
| description | The first 92 amino acids of VP2 of rotavirus are dispensable for VLP assembly and can be replaced by heterologous reporter proteins without affecting either self-assembly of chimeric VP2 or the interaction with VP6 to render chimeric VP2/6 VLPs. In this study, we constructed recombinant baculoviruses expressing GFP or OVA fused to the amino terminus of a truncated VP2 sequence and produced chimeric VLPs by co-infection with a baculovirus expressing VP6. The results showed that these chimeric VLPs were efficiently uptaken by murine dendritic cells and that the heterologous sequences contained in the core of these VLPs seemed to be able to reach the MHC-I pathway as they elicited strong and specific CTL responses. Therefore, the data presented here suggest that chimeric VLPs could be used as excellent carriers to elicit CTL responses to antigens transported inside the VLPs. |
| format | info:ar-repo/semantics/artículo |
| id | INTA4357 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA43572019-09-09T12:00:58Z Double-layered rotavirus-like particles are efficient carriers to elicit strong CTL responses to delivered heterologous antigens Molinari, Maria Paula Peralta, Andrea Veronica Maletto, Belkys Angélica Pistoresi Palencia, Maria Cristina Crespo, Maria Ines Moron, Victor Gabriel Taboga, Oscar Alberto Rotavirus Vacuna Antígenos Baculovirus Virus Vacuna Sintética Vaccines Antigens Viruses Synthetic Vaccines Organismos Virales Vacuna Recombinante Recombinant Vaccines Viruslike Particles The first 92 amino acids of VP2 of rotavirus are dispensable for VLP assembly and can be replaced by heterologous reporter proteins without affecting either self-assembly of chimeric VP2 or the interaction with VP6 to render chimeric VP2/6 VLPs. In this study, we constructed recombinant baculoviruses expressing GFP or OVA fused to the amino terminus of a truncated VP2 sequence and produced chimeric VLPs by co-infection with a baculovirus expressing VP6. The results showed that these chimeric VLPs were efficiently uptaken by murine dendritic cells and that the heterologous sequences contained in the core of these VLPs seemed to be able to reach the MHC-I pathway as they elicited strong and specific CTL responses. Therefore, the data presented here suggest that chimeric VLPs could be used as excellent carriers to elicit CTL responses to antigens transported inside the VLPs. Instituto de Biotecnología Fil: Molinari, Maria Paula. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Peralta, Andrea Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Maletto, Belkys Angélica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina Fil: Pistoresi Palencia, Maria Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina Fil: Crespo, Maria Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina Fil: Moron, Victor Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina Fil: Taboga, Oscar Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2019-01-30T13:08:12Z 2019-01-30T13:08:12Z 2014-11 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.sciencedirect.com/science/article/pii/S1359511314004000 http://hdl.handle.net/20.500.12123/4357 1359-5113 https://doi.org/10.1016/j.procbio.2014.07.014 eng info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Process Biochemistry 49 (11) : 1929-1935 (November 2014) |
| spellingShingle | Rotavirus Vacuna Antígenos Baculovirus Virus Vacuna Sintética Vaccines Antigens Viruses Synthetic Vaccines Organismos Virales Vacuna Recombinante Recombinant Vaccines Viruslike Particles Molinari, Maria Paula Peralta, Andrea Veronica Maletto, Belkys Angélica Pistoresi Palencia, Maria Cristina Crespo, Maria Ines Moron, Victor Gabriel Taboga, Oscar Alberto Double-layered rotavirus-like particles are efficient carriers to elicit strong CTL responses to delivered heterologous antigens |
| title | Double-layered rotavirus-like particles are efficient carriers to elicit strong CTL responses to delivered heterologous antigens |
| title_full | Double-layered rotavirus-like particles are efficient carriers to elicit strong CTL responses to delivered heterologous antigens |
| title_fullStr | Double-layered rotavirus-like particles are efficient carriers to elicit strong CTL responses to delivered heterologous antigens |
| title_full_unstemmed | Double-layered rotavirus-like particles are efficient carriers to elicit strong CTL responses to delivered heterologous antigens |
| title_short | Double-layered rotavirus-like particles are efficient carriers to elicit strong CTL responses to delivered heterologous antigens |
| title_sort | double layered rotavirus like particles are efficient carriers to elicit strong ctl responses to delivered heterologous antigens |
| topic | Rotavirus Vacuna Antígenos Baculovirus Virus Vacuna Sintética Vaccines Antigens Viruses Synthetic Vaccines Organismos Virales Vacuna Recombinante Recombinant Vaccines Viruslike Particles |
| url | https://www.sciencedirect.com/science/article/pii/S1359511314004000 http://hdl.handle.net/20.500.12123/4357 https://doi.org/10.1016/j.procbio.2014.07.014 |
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