VP1 protein of Foot-and-mouth disease virus (FMDV) impairs baculovirus surface display
The Foot-and-mouth disease virus (FMDV) causes important economical losses in livestock farming. In order to develop a novel subunit vaccine against FMDV, we constructed recombinant baculoviruses that display the protein VP1 of FMDV on their surface, with either polar (fused to gp64) or nonpolar (fu...
| Autores principales: | , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2019
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| Materias: | |
| Acceso en línea: | https://www.sciencedirect.com/science/article/pii/S0168170213001123 http://hdl.handle.net/20.500.12123/4355 https://doi.org/10.1016/j.virusres.2013.03.018 |
| _version_ | 1855035307444928512 |
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| author | Peralta, Andrea Veronica Maroniche, Guillermo Andrés Alfonso, Victoria Molinari, Maria Paula Taboga, Oscar Alberto |
| author_browse | Alfonso, Victoria Maroniche, Guillermo Andrés Molinari, Maria Paula Peralta, Andrea Veronica Taboga, Oscar Alberto |
| author_facet | Peralta, Andrea Veronica Maroniche, Guillermo Andrés Alfonso, Victoria Molinari, Maria Paula Taboga, Oscar Alberto |
| author_sort | Peralta, Andrea Veronica |
| collection | INTA Digital |
| description | The Foot-and-mouth disease virus (FMDV) causes important economical losses in livestock farming. In order to develop a novel subunit vaccine against FMDV, we constructed recombinant baculoviruses that display the protein VP1 of FMDV on their surface, with either polar (fused to gp64) or nonpolar (fused to anchor membrane from VSV-G protein) distribution. Insect cells infected with the different recombinant baculoviruses expressed VP1 fusion protein to high levels. However, the recombinant VP1 protein was not carried by budded virions. Subcellular localization of VP1 revealed that the trafficking of the fusion protein to the cell plasma membrane was impaired. Our results suggest that VP1 contains cryptic domains that interfere with protein secretion and subsequent incorporation into budded baculoviruses. |
| format | info:ar-repo/semantics/artículo |
| id | INTA4355 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA43552019-09-09T12:02:19Z VP1 protein of Foot-and-mouth disease virus (FMDV) impairs baculovirus surface display Peralta, Andrea Veronica Maroniche, Guillermo Andrés Alfonso, Victoria Molinari, Maria Paula Taboga, Oscar Alberto Enfermedades de los Animales Virus Fiebre Aftosa Fiebre Aftosa Ganado Baculovirus Animal Diseases Aphthovirus Foot and Mouth Disease Livestock The Foot-and-mouth disease virus (FMDV) causes important economical losses in livestock farming. In order to develop a novel subunit vaccine against FMDV, we constructed recombinant baculoviruses that display the protein VP1 of FMDV on their surface, with either polar (fused to gp64) or nonpolar (fused to anchor membrane from VSV-G protein) distribution. Insect cells infected with the different recombinant baculoviruses expressed VP1 fusion protein to high levels. However, the recombinant VP1 protein was not carried by budded virions. Subcellular localization of VP1 revealed that the trafficking of the fusion protein to the cell plasma membrane was impaired. Our results suggest that VP1 contains cryptic domains that interfere with protein secretion and subsequent incorporation into budded baculoviruses. Instituto de Biotecnología Fil: Peralta, Andrea Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Maroniche, Guillermo Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Alfonso, Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Molinari, Maria Paula. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Taboga, Oscar Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2019-01-30T12:12:29Z 2019-01-30T12:12:29Z 2013-07 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.sciencedirect.com/science/article/pii/S0168170213001123 http://hdl.handle.net/20.500.12123/4355 0168-1702 https://doi.org/10.1016/j.virusres.2013.03.018 eng info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Virus Research 175 (1) : 87-90 (July 2013) |
| spellingShingle | Enfermedades de los Animales Virus Fiebre Aftosa Fiebre Aftosa Ganado Baculovirus Animal Diseases Aphthovirus Foot and Mouth Disease Livestock Peralta, Andrea Veronica Maroniche, Guillermo Andrés Alfonso, Victoria Molinari, Maria Paula Taboga, Oscar Alberto VP1 protein of Foot-and-mouth disease virus (FMDV) impairs baculovirus surface display |
| title | VP1 protein of Foot-and-mouth disease virus (FMDV) impairs baculovirus surface display |
| title_full | VP1 protein of Foot-and-mouth disease virus (FMDV) impairs baculovirus surface display |
| title_fullStr | VP1 protein of Foot-and-mouth disease virus (FMDV) impairs baculovirus surface display |
| title_full_unstemmed | VP1 protein of Foot-and-mouth disease virus (FMDV) impairs baculovirus surface display |
| title_short | VP1 protein of Foot-and-mouth disease virus (FMDV) impairs baculovirus surface display |
| title_sort | vp1 protein of foot and mouth disease virus fmdv impairs baculovirus surface display |
| topic | Enfermedades de los Animales Virus Fiebre Aftosa Fiebre Aftosa Ganado Baculovirus Animal Diseases Aphthovirus Foot and Mouth Disease Livestock |
| url | https://www.sciencedirect.com/science/article/pii/S0168170213001123 http://hdl.handle.net/20.500.12123/4355 https://doi.org/10.1016/j.virusres.2013.03.018 |
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