Detection of bovine herpesvirus 2 and bovine herpesvirus 4 DNA in trigeminal ganglia of naturally infected cattle by polymerase chain reaction
Establishment of latent infection within specific tissues in the host is a common biological feature of the herpesviruses. In the case of bovine herpesvirus 2 (BoHV-2), latency is established in neuronal tissues, while bovine herpesvirus 4 (BoHV-4) and ovine herpesvirus 2 (OvHV-2) latent virus targe...
| Autores principales: | , , , , , , , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
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Elsevier
2019
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| Materias: | |
| Acceso en línea: | https://www.sciencedirect.com/science/article/pii/S037811351400162X http://hdl.handle.net/20.500.12123/4345 https://doi.org/10.1016/j.vetmic.2014.03.012 |
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| author | Campos, Fabrício Souza Franco, Ana Cláudia Oliveira, Martha Trindade Firpo, R.M. Strelczuk, G. Fontoura, F.E. Kulma, Marcos Iuri Roos Maidana, Silvina Soledad Romera, Sonia Alejandra Spilki, Fernando Silva, Alessandra D. Avila Hübner, Sílvia De Oliveira Roehe, Paulo Michel |
| author_browse | Campos, Fabrício Souza Firpo, R.M. Fontoura, F.E. Franco, Ana Cláudia Hübner, Sílvia De Oliveira Kulma, Marcos Iuri Roos Maidana, Silvina Soledad Oliveira, Martha Trindade Roehe, Paulo Michel Romera, Sonia Alejandra Silva, Alessandra D. Avila Spilki, Fernando Strelczuk, G. |
| author_facet | Campos, Fabrício Souza Franco, Ana Cláudia Oliveira, Martha Trindade Firpo, R.M. Strelczuk, G. Fontoura, F.E. Kulma, Marcos Iuri Roos Maidana, Silvina Soledad Romera, Sonia Alejandra Spilki, Fernando Silva, Alessandra D. Avila Hübner, Sílvia De Oliveira Roehe, Paulo Michel |
| author_sort | Campos, Fabrício Souza |
| collection | INTA Digital |
| description | Establishment of latent infection within specific tissues in the host is a common biological feature of the herpesviruses. In the case of bovine herpesvirus 2 (BoHV-2), latency is established in neuronal tissues, while bovine herpesvirus 4 (BoHV-4) and ovine herpesvirus 2 (OvHV-2) latent virus targets on cells of the monocytic lineage. This study was conducted in quest of BoHV-2, BoHV-4 and OvHV-2 DNA in two hundred trigeminal ganglia (TG) specimens, derived from one hundred clinically healthy cattle, majority of them naturally infected with bovine herpesvirus 1 (BoHV-1) and bovine herpesvirus 5 (BoHV-5). Total DNA extracted from ganglia was analyzed by polymerase chain reaction (PCR) designed to amplify part of the genes coding for BoHV-2, and BoHV-4 glycoprotein B and, for OvHV-2, the gene coding for phosphoribosylformylglycinamidine synthase-like protein. BoHV-2 DNA was detected in TG samples of two (2%) and BoHV-4 DNA in nine (9%) of the animals, whereas OvHV-2 DNA could not be detected in any of the TG DNA. The two animals in which BoHV-2 DNA was identified were also co-infected with BoHV-1 and BoHV-5. Within the nine animals in which BoHV-4 DNA was detected, six were also co-infected with BoHV-1 and BoHV-5. This report provides for the first time evidence that viral DNA from BoHV-2 and BoHV-4 can be occasionally detected in TG of naturally infected cattle. Likewise, in this report we provided for the first time evidence that the co-infection of cattle with three distinct bovine herpesviruses might be a naturally occurring phenomenon. |
| format | info:ar-repo/semantics/artículo |
| id | INTA4345 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA43452022-01-14T14:09:38Z Detection of bovine herpesvirus 2 and bovine herpesvirus 4 DNA in trigeminal ganglia of naturally infected cattle by polymerase chain reaction Campos, Fabrício Souza Franco, Ana Cláudia Oliveira, Martha Trindade Firpo, R.M. Strelczuk, G. Fontoura, F.E. Kulma, Marcos Iuri Roos Maidana, Silvina Soledad Romera, Sonia Alejandra Spilki, Fernando Silva, Alessandra D. Avila Hübner, Sílvia De Oliveira Roehe, Paulo Michel Enfermedades de los Animales Herpes Virus Bovino Ganado Bovino Diagnóstico Infecciones Latentes ADN Animal Diseases Bovine Herpesvirus Cattle Diagnosis Latent Infections DNA Establishment of latent infection within specific tissues in the host is a common biological feature of the herpesviruses. In the case of bovine herpesvirus 2 (BoHV-2), latency is established in neuronal tissues, while bovine herpesvirus 4 (BoHV-4) and ovine herpesvirus 2 (OvHV-2) latent virus targets on cells of the monocytic lineage. This study was conducted in quest of BoHV-2, BoHV-4 and OvHV-2 DNA in two hundred trigeminal ganglia (TG) specimens, derived from one hundred clinically healthy cattle, majority of them naturally infected with bovine herpesvirus 1 (BoHV-1) and bovine herpesvirus 5 (BoHV-5). Total DNA extracted from ganglia was analyzed by polymerase chain reaction (PCR) designed to amplify part of the genes coding for BoHV-2, and BoHV-4 glycoprotein B and, for OvHV-2, the gene coding for phosphoribosylformylglycinamidine synthase-like protein. BoHV-2 DNA was detected in TG samples of two (2%) and BoHV-4 DNA in nine (9%) of the animals, whereas OvHV-2 DNA could not be detected in any of the TG DNA. The two animals in which BoHV-2 DNA was identified were also co-infected with BoHV-1 and BoHV-5. Within the nine animals in which BoHV-4 DNA was detected, six were also co-infected with BoHV-1 and BoHV-5. This report provides for the first time evidence that viral DNA from BoHV-2 and BoHV-4 can be occasionally detected in TG of naturally infected cattle. Likewise, in this report we provided for the first time evidence that the co-infection of cattle with three distinct bovine herpesviruses might be a naturally occurring phenomenon. Instituto de Virología Fil: Campos, Fabrício Souza. Universidade Federal do Rio Grande do Sul. Institute of Basic Health Sciences. Department of Microbiology, Immunology and Parasitology. Laboratory of Virology; Brasil Fil: Franco, Ana Cláudia. Universidade Federal do Rio Grande do Sul. Institute of Basic Health Sciences. Department of Microbiology, Immunology and Parasitology. Laboratory of Virology; Brasil Fil: Oliveira, Martha Trindade. Universidade Federal do Rio Grande do Sul. Institute of Basic Health Sciences. Department of Microbiology, Immunology and Parasitology. Laboratory of Virology; Brasil Fil: Firpo, R.M. Universidade Federal do Rio Grande do Sul. Institute of Basic Health Sciences. Department of Microbiology, Immunology and Parasitology. Laboratory of Virology; Brasil Fil: Strelczuk, G. Universidade Federal do Rio Grande do Sul. Institute of Basic Health Sciences. Department of Microbiology, Immunology and Parasitology. Laboratory of Virology; Brasil Fil: Fontoura, F.E. Universidade Federal do Rio Grande do Sul. Institute of Basic Health Sciences. Department of Microbiology, Immunology and Parasitology. Laboratory of Virology; Brasil Fil: Kulma, Marcos Iuri Roos. Universidade Federal do Rio Grande do Sul. Institute of Basic Health Sciences. Department of Microbiology, Immunology and Parasitology. Laboratory of Virology; Brasil Fil: Maidana, Silvina Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Romera, Sonia Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Spilki, F. R. Universidade Feevale. Institute of Health Sciences. Molecular Microbiology Laboratory; Brasil Fil: Silva, Alessandra D. Avila. Empresa Brasileira de Pesquisa Agropecuaria (EMBRAPA); Brasil Fil: Hübner, Sílvia De Oliveira. Universidade Federal de Pelotas. Faculty of Veterinary Medicine. Department of Preventive Veterinary Medicine. Laboratory of Virology; Brasil Fil: Roehe, Paulo. Universidade Federal do Rio Grande do Sul. Institute of Basic Health Sciences. Department of Microbiology, Immunology and Parasitology. Laboratory of Virology; Brasil. Institute for Veterinary Research “Desidério Finamor” (IPVDF); Brasil 2019-01-29T12:43:19Z 2019-01-29T12:43:19Z 2014-06-25 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.sciencedirect.com/science/article/pii/S037811351400162X http://hdl.handle.net/20.500.12123/4345 0378-1135 1873-2542 https://doi.org/10.1016/j.vetmic.2014.03.012 eng info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Veterinary Microbiology 171 (1–2) : 182-188 (June 2014) |
| spellingShingle | Enfermedades de los Animales Herpes Virus Bovino Ganado Bovino Diagnóstico Infecciones Latentes ADN Animal Diseases Bovine Herpesvirus Cattle Diagnosis Latent Infections DNA Campos, Fabrício Souza Franco, Ana Cláudia Oliveira, Martha Trindade Firpo, R.M. Strelczuk, G. Fontoura, F.E. Kulma, Marcos Iuri Roos Maidana, Silvina Soledad Romera, Sonia Alejandra Spilki, Fernando Silva, Alessandra D. Avila Hübner, Sílvia De Oliveira Roehe, Paulo Michel Detection of bovine herpesvirus 2 and bovine herpesvirus 4 DNA in trigeminal ganglia of naturally infected cattle by polymerase chain reaction |
| title | Detection of bovine herpesvirus 2 and bovine herpesvirus 4 DNA in trigeminal ganglia of naturally infected cattle by polymerase chain reaction |
| title_full | Detection of bovine herpesvirus 2 and bovine herpesvirus 4 DNA in trigeminal ganglia of naturally infected cattle by polymerase chain reaction |
| title_fullStr | Detection of bovine herpesvirus 2 and bovine herpesvirus 4 DNA in trigeminal ganglia of naturally infected cattle by polymerase chain reaction |
| title_full_unstemmed | Detection of bovine herpesvirus 2 and bovine herpesvirus 4 DNA in trigeminal ganglia of naturally infected cattle by polymerase chain reaction |
| title_short | Detection of bovine herpesvirus 2 and bovine herpesvirus 4 DNA in trigeminal ganglia of naturally infected cattle by polymerase chain reaction |
| title_sort | detection of bovine herpesvirus 2 and bovine herpesvirus 4 dna in trigeminal ganglia of naturally infected cattle by polymerase chain reaction |
| topic | Enfermedades de los Animales Herpes Virus Bovino Ganado Bovino Diagnóstico Infecciones Latentes ADN Animal Diseases Bovine Herpesvirus Cattle Diagnosis Latent Infections DNA |
| url | https://www.sciencedirect.com/science/article/pii/S037811351400162X http://hdl.handle.net/20.500.12123/4345 https://doi.org/10.1016/j.vetmic.2014.03.012 |
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