Accuracy of traditional and novel serology tests for predicting cross-protection in foot-and-mouth disease vaccinated cattle
Foot-and-mouth disease virus (FMDV) antigenic-match between vaccine and field viruses has traditionally been estimated in vitro by computing the r1 value using virus neutralization test (VNT) or ELISA titers. In this study we compared the accuracy in predicting cross-protection between the r1 value...
| Autores principales: | , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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Elsevier
2019
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| Acceso en línea: | https://www.sciencedirect.com/science/article/pii/S0264410X13017052 http://hdl.handle.net/20.500.12123/4342 https://doi.org/10.1016/j.vaccine.2013.12.007 |
| _version_ | 1855483414219587584 |
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| author | Brito, Barbara Patricia Perez, Andrés Maximiliano Capozzo, Alejandra Victoria |
| author_browse | Brito, Barbara Patricia Capozzo, Alejandra Victoria Perez, Andrés Maximiliano |
| author_facet | Brito, Barbara Patricia Perez, Andrés Maximiliano Capozzo, Alejandra Victoria |
| author_sort | Brito, Barbara Patricia |
| collection | INTA Digital |
| description | Foot-and-mouth disease virus (FMDV) antigenic-match between vaccine and field viruses has traditionally been estimated in vitro by computing the r1 value using virus neutralization test (VNT) or ELISA titers. In this study we compared the accuracy in predicting cross-protection between the r1 value estimated by VNT and two recently developed tests that measure IgG subtypes and avidity. Data analyzed consisted of 64 serum samples from FMDV A24/Cruzeiro vaccinated bovines challenged with the heterologous A/Argentina/2001 strain and evaluated for podal generalization. We computed the tests sensitivity (Se), specificity (Sp), and receiving operating characteristics (ROC) curve. The heterologous IgG1/IgG2 ratio was the most accurate test (Se = 0.71, Sp = 0.98), followed by heterologous IgG1 (Se = 0.53, Sp = 0.96), VNT (Se = 0.47, Sp = 1.00), whereas r1 accuracy was substantially low (Se = 0.41, Sp = 0.81). Because sensitivity of individual tests was limited, we argue that two or more of the tests should be used in combination to produce accurate estimates of protection. |
| format | Artículo |
| id | INTA4342 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA43422019-06-05T18:23:28Z Accuracy of traditional and novel serology tests for predicting cross-protection in foot-and-mouth disease vaccinated cattle Brito, Barbara Patricia Perez, Andrés Maximiliano Capozzo, Alejandra Victoria Ganado Bovino Enfermedades de los Animales Virus Fiebre Aftosa Fiebre Aftosa Serología Vacuna Cattle Animal Diseases Aphthovirus Foot and Mouth Disease Serology Vaccines Foot-and-mouth disease virus (FMDV) antigenic-match between vaccine and field viruses has traditionally been estimated in vitro by computing the r1 value using virus neutralization test (VNT) or ELISA titers. In this study we compared the accuracy in predicting cross-protection between the r1 value estimated by VNT and two recently developed tests that measure IgG subtypes and avidity. Data analyzed consisted of 64 serum samples from FMDV A24/Cruzeiro vaccinated bovines challenged with the heterologous A/Argentina/2001 strain and evaluated for podal generalization. We computed the tests sensitivity (Se), specificity (Sp), and receiving operating characteristics (ROC) curve. The heterologous IgG1/IgG2 ratio was the most accurate test (Se = 0.71, Sp = 0.98), followed by heterologous IgG1 (Se = 0.53, Sp = 0.96), VNT (Se = 0.47, Sp = 1.00), whereas r1 accuracy was substantially low (Se = 0.41, Sp = 0.81). Because sensitivity of individual tests was limited, we argue that two or more of the tests should be used in combination to produce accurate estimates of protection. Instituto de Virología Fil: Brito, Bárbara Patricia. University of California-Davis. Center for Animal Diseases Modeling and Surveillance; Estados Unidos Fil: Perez, Andrés Maximiliano. University of California-Davis. Center for Animal Diseases Modeling and Surveillance; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Capozzo, Alejandra Victoria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2019-01-28T16:59:13Z 2019-01-28T16:59:13Z 2014-01-16 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.sciencedirect.com/science/article/pii/S0264410X13017052 http://hdl.handle.net/20.500.12123/4342 0264-410X https://doi.org/10.1016/j.vaccine.2013.12.007 eng info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Vaccine 32 (4) : 433-436 (January 2014) |
| spellingShingle | Ganado Bovino Enfermedades de los Animales Virus Fiebre Aftosa Fiebre Aftosa Serología Vacuna Cattle Animal Diseases Aphthovirus Foot and Mouth Disease Serology Vaccines Brito, Barbara Patricia Perez, Andrés Maximiliano Capozzo, Alejandra Victoria Accuracy of traditional and novel serology tests for predicting cross-protection in foot-and-mouth disease vaccinated cattle |
| title | Accuracy of traditional and novel serology tests for predicting cross-protection in foot-and-mouth disease vaccinated cattle |
| title_full | Accuracy of traditional and novel serology tests for predicting cross-protection in foot-and-mouth disease vaccinated cattle |
| title_fullStr | Accuracy of traditional and novel serology tests for predicting cross-protection in foot-and-mouth disease vaccinated cattle |
| title_full_unstemmed | Accuracy of traditional and novel serology tests for predicting cross-protection in foot-and-mouth disease vaccinated cattle |
| title_short | Accuracy of traditional and novel serology tests for predicting cross-protection in foot-and-mouth disease vaccinated cattle |
| title_sort | accuracy of traditional and novel serology tests for predicting cross protection in foot and mouth disease vaccinated cattle |
| topic | Ganado Bovino Enfermedades de los Animales Virus Fiebre Aftosa Fiebre Aftosa Serología Vacuna Cattle Animal Diseases Aphthovirus Foot and Mouth Disease Serology Vaccines |
| url | https://www.sciencedirect.com/science/article/pii/S0264410X13017052 http://hdl.handle.net/20.500.12123/4342 https://doi.org/10.1016/j.vaccine.2013.12.007 |
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