Brucella spp. Lumazine synthase as a novel immunomodulator to produce egg yolk antibodies
Lumazine synthase from Brucella spp. (BLS) is a highly immunogenic decameric protein. It has been previously described as a carrier of peptides or proteins to increase their immunogenicity in different animal species, but its activity has never been evaluated in chickens. In this work, the use of BL...
| Autores principales: | , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Scientific Research
2019
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| Materias: | |
| Acceso en línea: | https://www.scirp.org/journal/PaperInformation.aspx?PaperID=17200 http://hdl.handle.net/20.500.12123/4341 http://dx.doi.org/10.4236/abb.2012.31012 |
| _version_ | 1855483414040281088 |
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| author | Bellido, Demian Chacana, Pablo Mozgovoj, Marina Valeria Gonzalez, Diego Goldbaum, Fernando Alberto Wigdorovitz, Andres Dus Santos, Maria Jose |
| author_browse | Bellido, Demian Chacana, Pablo Dus Santos, Maria Jose Goldbaum, Fernando Alberto Gonzalez, Diego Mozgovoj, Marina Valeria Wigdorovitz, Andres |
| author_facet | Bellido, Demian Chacana, Pablo Mozgovoj, Marina Valeria Gonzalez, Diego Goldbaum, Fernando Alberto Wigdorovitz, Andres Dus Santos, Maria Jose |
| author_sort | Bellido, Demian |
| collection | INTA Digital |
| description | Lumazine synthase from Brucella spp. (BLS) is a highly immunogenic decameric protein. It has been previously described as a carrier of peptides or proteins to increase their immunogenicity in different animal species, but its activity has never been evaluated in chickens. In this work, the use of BLS to improve the antibody response against bovine rotavirus (BRV) VP8d protein in laying hens was assessed. VP8d is the inner domain of the VP8 spike protein which preserves the sialic acid binding activity and the neutralizing epitopes present in the viral protein. Hens were immunized three times with 2 μg of VP8d alone or fused to BLS. Hens inoculated with BLSVP8d developed higher antibody titers (evaluated by ELISA and viral neutralization test) than hens immunized either with VP8d alone or the mixture of VP8d and BLS. Furthermore, IgY antibodies against BLSVP8d were able to fully protect mice against challenge with virulent BRV in a dose-depent-manner. Overall, these results demonstrate that BLS is a potent immonumodulator that enhances the antibody response in hens, thus increasing the concentration of specific IgY in the egg yolk, one of the main issues to be adressed in order to improve the use of the IgY technology. |
| format | Artículo |
| id | INTA4341 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Scientific Research |
| publisherStr | Scientific Research |
| record_format | dspace |
| spelling | INTA43412019-01-28T16:05:25Z Brucella spp. Lumazine synthase as a novel immunomodulator to produce egg yolk antibodies Bellido, Demian Chacana, Pablo Mozgovoj, Marina Valeria Gonzalez, Diego Goldbaum, Fernando Alberto Wigdorovitz, Andres Dus Santos, Maria Jose Brucella Inmunogenética Proteínas Yema de Huevo Rotavirus Bovino Anticuerpos Immunogenetics Proteins Egg Yolk Bovine Rotavirus Antibodies Lumazine synthase from Brucella spp. (BLS) is a highly immunogenic decameric protein. It has been previously described as a carrier of peptides or proteins to increase their immunogenicity in different animal species, but its activity has never been evaluated in chickens. In this work, the use of BLS to improve the antibody response against bovine rotavirus (BRV) VP8d protein in laying hens was assessed. VP8d is the inner domain of the VP8 spike protein which preserves the sialic acid binding activity and the neutralizing epitopes present in the viral protein. Hens were immunized three times with 2 μg of VP8d alone or fused to BLS. Hens inoculated with BLSVP8d developed higher antibody titers (evaluated by ELISA and viral neutralization test) than hens immunized either with VP8d alone or the mixture of VP8d and BLS. Furthermore, IgY antibodies against BLSVP8d were able to fully protect mice against challenge with virulent BRV in a dose-depent-manner. Overall, these results demonstrate that BLS is a potent immonumodulator that enhances the antibody response in hens, thus increasing the concentration of specific IgY in the egg yolk, one of the main issues to be adressed in order to improve the use of the IgY technology. Instituto de Virología Fil: Bellido, Demian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Chacana, Pablo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Mozgovoj, Marina Valeria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Gonzalez, Diego. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Goldbaum, Fernando Alberto. Fundación Instituto Leloir; Argentina Fil: Wigdorovitz, Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Dus Santos, Maria Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina 2019-01-28T15:48:56Z 2019-01-28T15:48:56Z 2012-02 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.scirp.org/journal/PaperInformation.aspx?PaperID=17200 http://hdl.handle.net/20.500.12123/4341 2156-8456 2156-8502 http://dx.doi.org/10.4236/abb.2012.31012 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Scientific Research Advances in Bioscience and Biotechnology 3 (1) : 80-86 (February 2012) |
| spellingShingle | Brucella Inmunogenética Proteínas Yema de Huevo Rotavirus Bovino Anticuerpos Immunogenetics Proteins Egg Yolk Bovine Rotavirus Antibodies Bellido, Demian Chacana, Pablo Mozgovoj, Marina Valeria Gonzalez, Diego Goldbaum, Fernando Alberto Wigdorovitz, Andres Dus Santos, Maria Jose Brucella spp. Lumazine synthase as a novel immunomodulator to produce egg yolk antibodies |
| title | Brucella spp. Lumazine synthase as a novel immunomodulator to produce egg yolk antibodies |
| title_full | Brucella spp. Lumazine synthase as a novel immunomodulator to produce egg yolk antibodies |
| title_fullStr | Brucella spp. Lumazine synthase as a novel immunomodulator to produce egg yolk antibodies |
| title_full_unstemmed | Brucella spp. Lumazine synthase as a novel immunomodulator to produce egg yolk antibodies |
| title_short | Brucella spp. Lumazine synthase as a novel immunomodulator to produce egg yolk antibodies |
| title_sort | brucella spp lumazine synthase as a novel immunomodulator to produce egg yolk antibodies |
| topic | Brucella Inmunogenética Proteínas Yema de Huevo Rotavirus Bovino Anticuerpos Immunogenetics Proteins Egg Yolk Bovine Rotavirus Antibodies |
| url | https://www.scirp.org/journal/PaperInformation.aspx?PaperID=17200 http://hdl.handle.net/20.500.12123/4341 http://dx.doi.org/10.4236/abb.2012.31012 |
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