Fine mapping of LrSV2, a race-specific adult plant leaf rust resistance gene on wheat chromosome 3BS
Three leaf rust resistance genes were identified in the durable leaf rust resistant Argentinean wheat variety Sinvalocho MA: the seedling resistance gene Lr3 on distal 6BL and two adult plant resistance genes, LrSV1 and LrSV2, on chromosomes 2DS and 3BS, respectively. To develop a high-resolution ge...
| Autores principales: | , , , , , , , , |
|---|---|
| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
Springer
2019
|
| Materias: | |
| Acceso en línea: | https://link.springer.com/article/10.1007/s00122-014-2285-z http://hdl.handle.net/20.500.12123/4333 https://doi.org/10.1007/s00122-014-2285-z |
| _version_ | 1855035303205535744 |
|---|---|
| author | Dieguez, Maria Jose Pergolesi, María Fernanda Velásquez, Silvia Melina Ingala, Lorena Romina López, M. Darino, Martin Alejandro Paux, Etienne Feuillet, Catherine Sacco, Francisco |
| author_browse | Darino, Martin Alejandro Dieguez, Maria Jose Feuillet, Catherine Ingala, Lorena Romina López, M. Paux, Etienne Pergolesi, María Fernanda Sacco, Francisco Velásquez, Silvia Melina |
| author_facet | Dieguez, Maria Jose Pergolesi, María Fernanda Velásquez, Silvia Melina Ingala, Lorena Romina López, M. Darino, Martin Alejandro Paux, Etienne Feuillet, Catherine Sacco, Francisco |
| author_sort | Dieguez, Maria Jose |
| collection | INTA Digital |
| description | Three leaf rust resistance genes were identified in the durable leaf rust resistant Argentinean wheat variety Sinvalocho MA: the seedling resistance gene Lr3 on distal 6BL and two adult plant resistance genes, LrSV1 and LrSV2, on chromosomes 2DS and 3BS, respectively. To develop a high-resolution genetic map for LrSV2, 10 markers were genotyped on 343 F2 individuals from a cross between Sinvalocho MA and Gama6. The closest co-dominant markers on both sides of the gene (3 microsatellites and 2 STMs) were analyzed on 965 additional F2s from the same cross. Microsatellite marker cfb5010 cosegregated with LrSV2 whereas flanking markers were found at 1 cM distal and at 0.3 cM proximal to the gene. SSR markers designed from the sequences of cv Chinese Spring BAC clones spanning the LrSV2 genetic interval were tested on the recombinants, allowing the identification of microsatellite swm13 at 0.15 cM distal to LrSV2. This delimited an interval of 0.45 cM around the gene flanked by the SSR markers swm13 and gwm533 at the subtelomeric end of chromosome 3BS. |
| format | info:ar-repo/semantics/artículo |
| id | INTA4333 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA43332019-01-25T13:26:16Z Fine mapping of LrSV2, a race-specific adult plant leaf rust resistance gene on wheat chromosome 3BS Dieguez, Maria Jose Pergolesi, María Fernanda Velásquez, Silvia Melina Ingala, Lorena Romina López, M. Darino, Martin Alejandro Paux, Etienne Feuillet, Catherine Sacco, Francisco Trigo Enfermedades de las Plantas Roya Resistencia Genética Cromosomas Marcadores Genéticos Wheat Plant Diseases Rusts Genetic Resistance Chromosomes Genetic Markers Marcadores Moleculares Three leaf rust resistance genes were identified in the durable leaf rust resistant Argentinean wheat variety Sinvalocho MA: the seedling resistance gene Lr3 on distal 6BL and two adult plant resistance genes, LrSV1 and LrSV2, on chromosomes 2DS and 3BS, respectively. To develop a high-resolution genetic map for LrSV2, 10 markers were genotyped on 343 F2 individuals from a cross between Sinvalocho MA and Gama6. The closest co-dominant markers on both sides of the gene (3 microsatellites and 2 STMs) were analyzed on 965 additional F2s from the same cross. Microsatellite marker cfb5010 cosegregated with LrSV2 whereas flanking markers were found at 1 cM distal and at 0.3 cM proximal to the gene. SSR markers designed from the sequences of cv Chinese Spring BAC clones spanning the LrSV2 genetic interval were tested on the recombinants, allowing the identification of microsatellite swm13 at 0.15 cM distal to LrSV2. This delimited an interval of 0.45 cM around the gene flanked by the SSR markers swm13 and gwm533 at the subtelomeric end of chromosome 3BS. Instituto de Genética Fil: Dieguez, María José. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Pergolesi, María Fernanda. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Velásquez, Silvia Melina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina Fil: Ingala, Lorena Romina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: López, M. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Darino, Martín Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Paux, Etienne. Institut National de la Recherche Agronomique. UMR Genetics, Diversity and Ecophysiology of Cereals; Francia Fil: Feuillet, Catherine. Institut National de la Recherche Agronomique. UMR Genetics, Diversity and Ecophysiology of Cereals; Francia Fil: Sacco, Francisco. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina 2019-01-25T13:24:09Z 2019-01-25T13:24:09Z 2014-05 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://link.springer.com/article/10.1007/s00122-014-2285-z http://hdl.handle.net/20.500.12123/4333 0040-5752 1432-2242 https://doi.org/10.1007/s00122-014-2285-z eng info:eu-repo/semantics/restrictedAccess application/pdf Springer Theoretical and Applied Genetics 127 (5) : 1133–1141 (May 2014) |
| spellingShingle | Trigo Enfermedades de las Plantas Roya Resistencia Genética Cromosomas Marcadores Genéticos Wheat Plant Diseases Rusts Genetic Resistance Chromosomes Genetic Markers Marcadores Moleculares Dieguez, Maria Jose Pergolesi, María Fernanda Velásquez, Silvia Melina Ingala, Lorena Romina López, M. Darino, Martin Alejandro Paux, Etienne Feuillet, Catherine Sacco, Francisco Fine mapping of LrSV2, a race-specific adult plant leaf rust resistance gene on wheat chromosome 3BS |
| title | Fine mapping of LrSV2, a race-specific adult plant leaf rust resistance gene on wheat chromosome 3BS |
| title_full | Fine mapping of LrSV2, a race-specific adult plant leaf rust resistance gene on wheat chromosome 3BS |
| title_fullStr | Fine mapping of LrSV2, a race-specific adult plant leaf rust resistance gene on wheat chromosome 3BS |
| title_full_unstemmed | Fine mapping of LrSV2, a race-specific adult plant leaf rust resistance gene on wheat chromosome 3BS |
| title_short | Fine mapping of LrSV2, a race-specific adult plant leaf rust resistance gene on wheat chromosome 3BS |
| title_sort | fine mapping of lrsv2 a race specific adult plant leaf rust resistance gene on wheat chromosome 3bs |
| topic | Trigo Enfermedades de las Plantas Roya Resistencia Genética Cromosomas Marcadores Genéticos Wheat Plant Diseases Rusts Genetic Resistance Chromosomes Genetic Markers Marcadores Moleculares |
| url | https://link.springer.com/article/10.1007/s00122-014-2285-z http://hdl.handle.net/20.500.12123/4333 https://doi.org/10.1007/s00122-014-2285-z |
| work_keys_str_mv | AT dieguezmariajose finemappingoflrsv2aracespecificadultplantleafrustresistancegeneonwheatchromosome3bs AT pergolesimariafernanda finemappingoflrsv2aracespecificadultplantleafrustresistancegeneonwheatchromosome3bs AT velasquezsilviamelina finemappingoflrsv2aracespecificadultplantleafrustresistancegeneonwheatchromosome3bs AT ingalalorenaromina finemappingoflrsv2aracespecificadultplantleafrustresistancegeneonwheatchromosome3bs AT lopezm finemappingoflrsv2aracespecificadultplantleafrustresistancegeneonwheatchromosome3bs AT darinomartinalejandro finemappingoflrsv2aracespecificadultplantleafrustresistancegeneonwheatchromosome3bs AT pauxetienne finemappingoflrsv2aracespecificadultplantleafrustresistancegeneonwheatchromosome3bs AT feuilletcatherine finemappingoflrsv2aracespecificadultplantleafrustresistancegeneonwheatchromosome3bs AT saccofrancisco finemappingoflrsv2aracespecificadultplantleafrustresistancegeneonwheatchromosome3bs |