Effect of gaseous atmosphere on different supplementation medium for In Vitro canine oocyte maturation
The aim of the present research was to verify the influence of two different atmosphere gases (A: 5%CO2, B: 5%CO2, 5%O2 and 90%N2) on three different maturation media on in vitro maturation (IVM) rates for oocytes from anoestrous and diestrous bitches that were cultured in vitro for 48h. Oocytes har...
| Autores principales: | , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
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Bentham Open
2019
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| Materias: | |
| Acceso en línea: | https://benthamopen.com/contents/pdf/TOVSJ/TOVSJ-8-1.pdf http://hdl.handle.net/20.500.12123/4331 https://doi.org/10.2174/1874318801408010001 |
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| author | González, Noelia Gil, María Lydia Rios, Glenda Laura Canizo, Jésica Romina De Blas, José Ignacio Alberio, Ricardo |
| author_browse | Alberio, Ricardo Canizo, Jésica Romina De Blas, José Ignacio Gil, María Lydia González, Noelia Rios, Glenda Laura |
| author_facet | González, Noelia Gil, María Lydia Rios, Glenda Laura Canizo, Jésica Romina De Blas, José Ignacio Alberio, Ricardo |
| author_sort | González, Noelia |
| collection | INTA Digital |
| description | The aim of the present research was to verify the influence of two different atmosphere gases (A: 5%CO2, B: 5%CO2, 5%O2 and 90%N2) on three different maturation media on in vitro maturation (IVM) rates for oocytes from anoestrous and diestrous bitches that were cultured in vitro for 48h. Oocytes harvested from bitches were selected and allocated into three maturation media groups, cultured in atmosphere A or B. Group 1, oestrous bitch serum; Group 2, bovine serum albumin (BSA), glutamine, cisteamine; Group 3, BSA, estrogen (E2), epidermal growth factor (EGF). Progression of nuclear maturation was evaluated under fluorescent microscopy after staining with Hoechst 33342 solution.
The results demonstrated that different maturation media was not influenced by the atmosphere in any case. Group 1 showed the lowest maturation rates in both cases: 4.3±1.3 vs 7.3±2.1 and 7.9±2.5 for groups 1, 2 and 3 respectively in atmosphere A (p≤ 0.5), 0 vs 7.2± 2.9 and 9.3± 4.0 in atmosphere B (p= 0.33). Maturation rates were similar among treatments 2 and 3 in both atmospheres, were slightly higher for treatment 3. Moreover, group 1 obtained the highest degeneration rates: 65.5±7.3 vs 39.63±7.1, 28.19±6.4 (p= 0.018) and 71.43±12.2 vs 27.71±10, 25.33± 10.20 (p= 0.010) for mediums 1, 2, and 3 in atmospheres A and B respectively.
Our findings indicate that atmosphere B seems to be more favorable habitat for canine oocytes and independently of gassed, maturation with not controlled supplementation, with estrous bitch serum, is not a good option. |
| format | info:ar-repo/semantics/artículo |
| id | INTA4331 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Bentham Open |
| publisherStr | Bentham Open |
| record_format | dspace |
| spelling | INTA43312019-03-26T11:56:04Z Effect of gaseous atmosphere on different supplementation medium for In Vitro canine oocyte maturation González, Noelia Gil, María Lydia Rios, Glenda Laura Canizo, Jésica Romina De Blas, José Ignacio Alberio, Ricardo Óvulo Perro Experimentación in Vitro Ova Dogs In Vitro Experimentation Ovocitos Atmósfera Gaseosa The aim of the present research was to verify the influence of two different atmosphere gases (A: 5%CO2, B: 5%CO2, 5%O2 and 90%N2) on three different maturation media on in vitro maturation (IVM) rates for oocytes from anoestrous and diestrous bitches that were cultured in vitro for 48h. Oocytes harvested from bitches were selected and allocated into three maturation media groups, cultured in atmosphere A or B. Group 1, oestrous bitch serum; Group 2, bovine serum albumin (BSA), glutamine, cisteamine; Group 3, BSA, estrogen (E2), epidermal growth factor (EGF). Progression of nuclear maturation was evaluated under fluorescent microscopy after staining with Hoechst 33342 solution. The results demonstrated that different maturation media was not influenced by the atmosphere in any case. Group 1 showed the lowest maturation rates in both cases: 4.3±1.3 vs 7.3±2.1 and 7.9±2.5 for groups 1, 2 and 3 respectively in atmosphere A (p≤ 0.5), 0 vs 7.2± 2.9 and 9.3± 4.0 in atmosphere B (p= 0.33). Maturation rates were similar among treatments 2 and 3 in both atmospheres, were slightly higher for treatment 3. Moreover, group 1 obtained the highest degeneration rates: 65.5±7.3 vs 39.63±7.1, 28.19±6.4 (p= 0.018) and 71.43±12.2 vs 27.71±10, 25.33± 10.20 (p= 0.010) for mediums 1, 2, and 3 in atmospheres A and B respectively. Our findings indicate that atmosphere B seems to be more favorable habitat for canine oocytes and independently of gassed, maturation with not controlled supplementation, with estrous bitch serum, is not a good option. EEA Balcarce Fil: González, Noelia. Universidad de Zaragoza. Facultad de Veterinaria. Reproduction and Obstetric Unit; España Fil: Gil, Lydia. Universidad de Zaragoza. Facultad de Veterinaria. Reproduction and Obstetric Unit; España Fil: Rios, Glenda Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Departamento de Producción Animal. Biotecnología de la Reproducción; Argentina Fil: Canizo, Jésica Romina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Departamento de Producción Animal. Biotecnología de la Reproducción; Argentina Fil: De Blas, José Ignacio. Universidad de Zaragoza. Facultad de Veterinaria. Infectious Diseases Unit; España Fil: Alberio, Ricardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Departamento de Producción Animal. Biotecnología de la Reproducción; Argentina 2019-01-25T12:21:33Z 2019-01-25T12:21:33Z 2014-02 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://benthamopen.com/contents/pdf/TOVSJ/TOVSJ-8-1.pdf http://hdl.handle.net/20.500.12123/4331 1874-3188 https://doi.org/10.2174/1874318801408010001 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Bentham Open The Open Veterinary Science Journal 8 : 1-5 (2014) |
| spellingShingle | Óvulo Perro Experimentación in Vitro Ova Dogs In Vitro Experimentation Ovocitos Atmósfera Gaseosa González, Noelia Gil, María Lydia Rios, Glenda Laura Canizo, Jésica Romina De Blas, José Ignacio Alberio, Ricardo Effect of gaseous atmosphere on different supplementation medium for In Vitro canine oocyte maturation |
| title | Effect of gaseous atmosphere on different supplementation medium for In Vitro canine oocyte maturation |
| title_full | Effect of gaseous atmosphere on different supplementation medium for In Vitro canine oocyte maturation |
| title_fullStr | Effect of gaseous atmosphere on different supplementation medium for In Vitro canine oocyte maturation |
| title_full_unstemmed | Effect of gaseous atmosphere on different supplementation medium for In Vitro canine oocyte maturation |
| title_short | Effect of gaseous atmosphere on different supplementation medium for In Vitro canine oocyte maturation |
| title_sort | effect of gaseous atmosphere on different supplementation medium for in vitro canine oocyte maturation |
| topic | Óvulo Perro Experimentación in Vitro Ova Dogs In Vitro Experimentation Ovocitos Atmósfera Gaseosa |
| url | https://benthamopen.com/contents/pdf/TOVSJ/TOVSJ-8-1.pdf http://hdl.handle.net/20.500.12123/4331 https://doi.org/10.2174/1874318801408010001 |
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