Effect of Bovine Viral Diarrhea Virus on the ovarian functionality and in vitro reproductive performance of persistently infected heifers
The aim of this study was to study the effect of Bovine Viral Diarrhea Virus on the reproductive female tract by means of analyzing the ovarian follicular population of persistently infected (PI) heifers, and evaluating the performance of oocytes procured form those heifers in in vitro fertilization...
| Autores principales: | , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2019
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| Materias: | |
| Acceso en línea: | https://www.sciencedirect.com/science/article/pii/S0378113513002095 http://hdl.handle.net/20.500.12123/4329 https://doi.org/10.1016/j.vetmic.2013.04.007 |
| _version_ | 1855035302458949632 |
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| author | González Altamiranda, Erika Analía Kaiser, German Gustavo Mucci, Nicolas Crescencio Verna, Andrea Elizabeth Campero, Carlos Manuel Odeon, Anselmo Carlos |
| author_browse | Campero, Carlos Manuel González Altamiranda, Erika Analía Kaiser, German Gustavo Mucci, Nicolas Crescencio Odeon, Anselmo Carlos Verna, Andrea Elizabeth |
| author_facet | González Altamiranda, Erika Analía Kaiser, German Gustavo Mucci, Nicolas Crescencio Verna, Andrea Elizabeth Campero, Carlos Manuel Odeon, Anselmo Carlos |
| author_sort | González Altamiranda, Erika Analía |
| collection | INTA Digital |
| description | The aim of this study was to study the effect of Bovine Viral Diarrhea Virus on the reproductive female tract by means of analyzing the ovarian follicular population of persistently infected (PI) heifers, and evaluating the performance of oocytes procured form those heifers in in vitro fertilization procedures.
Seven BVDV PI Aberdeen Angus and British crossbred heifers ranging from 18 to 36 months of age were spayed and their ovaries used for viral isolation, microscopic examination, and in vitro fertilization procedures. Bovine Viral Diarrhea Virus was detected from the follicular fluid and sera of all PI heifers. Microscopic examination of the ovaries from PI heifers showed a significant drop in the number of follicles cortical regions, compared with controls. A comparative analysis of the stages of follicular development showed a significant decrease in the number of primordial and tertiary follicles in the cortical regions of ovaries from PI heifers. Viral antigen was detected by immunohistochemistry, and was widely distributed throughout the ovarian tissues. There were differences in the rate of cleavage and embryo development between oocytes obtained from the ovaries of control animals and PI heifers. Furthermore, two developed embryos obtained from oocytes from one of the PI heifers were positive to BVDV, as well as two media from in vitro fertilization (IVF) procedures. The results of this study demonstrate that BVDV PI heifers exhibit alterations in follicular population through of the early interaction between the virus and germ cell line affecting directly the mechanisms involved in the ontogenesis of the ovary. |
| format | info:ar-repo/semantics/artículo |
| id | INTA4329 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA43292019-06-10T18:32:56Z Effect of Bovine Viral Diarrhea Virus on the ovarian functionality and in vitro reproductive performance of persistently infected heifers González Altamiranda, Erika Analía Kaiser, German Gustavo Mucci, Nicolas Crescencio Verna, Andrea Elizabeth Campero, Carlos Manuel Odeon, Anselmo Carlos Novilla Enfermedades de los Animales Pestivirus de la Diarrea Bovina Diarrea Viral Bovina Folículos Ováricos Heifers Animal Diseases Bovine Diarrhoea Pestivirus Bovine Viral Diarrhoea Ovarian Follicles The aim of this study was to study the effect of Bovine Viral Diarrhea Virus on the reproductive female tract by means of analyzing the ovarian follicular population of persistently infected (PI) heifers, and evaluating the performance of oocytes procured form those heifers in in vitro fertilization procedures. Seven BVDV PI Aberdeen Angus and British crossbred heifers ranging from 18 to 36 months of age were spayed and their ovaries used for viral isolation, microscopic examination, and in vitro fertilization procedures. Bovine Viral Diarrhea Virus was detected from the follicular fluid and sera of all PI heifers. Microscopic examination of the ovaries from PI heifers showed a significant drop in the number of follicles cortical regions, compared with controls. A comparative analysis of the stages of follicular development showed a significant decrease in the number of primordial and tertiary follicles in the cortical regions of ovaries from PI heifers. Viral antigen was detected by immunohistochemistry, and was widely distributed throughout the ovarian tissues. There were differences in the rate of cleavage and embryo development between oocytes obtained from the ovaries of control animals and PI heifers. Furthermore, two developed embryos obtained from oocytes from one of the PI heifers were positive to BVDV, as well as two media from in vitro fertilization (IVF) procedures. The results of this study demonstrate that BVDV PI heifers exhibit alterations in follicular population through of the early interaction between the virus and germ cell line affecting directly the mechanisms involved in the ontogenesis of the ovary. EEA Balcarce Fil: Gonzalez Altamiranda, Erika Analia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Kaiser, German Gustavo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Mucci, Nicolas Crescencio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Verna, Andrea Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Campero, Carlos Manuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Odeon, Anselmo Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina 2019-01-25T11:15:06Z 2019-01-25T11:15:06Z 2013-08-30 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.sciencedirect.com/science/article/pii/S0378113513002095 http://hdl.handle.net/20.500.12123/4329 0378-1135 https://doi.org/10.1016/j.vetmic.2013.04.007 eng info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Veterinary Microbiology 165 (3–4) : 326-332 (August 2013) |
| spellingShingle | Novilla Enfermedades de los Animales Pestivirus de la Diarrea Bovina Diarrea Viral Bovina Folículos Ováricos Heifers Animal Diseases Bovine Diarrhoea Pestivirus Bovine Viral Diarrhoea Ovarian Follicles González Altamiranda, Erika Analía Kaiser, German Gustavo Mucci, Nicolas Crescencio Verna, Andrea Elizabeth Campero, Carlos Manuel Odeon, Anselmo Carlos Effect of Bovine Viral Diarrhea Virus on the ovarian functionality and in vitro reproductive performance of persistently infected heifers |
| title | Effect of Bovine Viral Diarrhea Virus on the ovarian functionality and in vitro reproductive performance of persistently infected heifers |
| title_full | Effect of Bovine Viral Diarrhea Virus on the ovarian functionality and in vitro reproductive performance of persistently infected heifers |
| title_fullStr | Effect of Bovine Viral Diarrhea Virus on the ovarian functionality and in vitro reproductive performance of persistently infected heifers |
| title_full_unstemmed | Effect of Bovine Viral Diarrhea Virus on the ovarian functionality and in vitro reproductive performance of persistently infected heifers |
| title_short | Effect of Bovine Viral Diarrhea Virus on the ovarian functionality and in vitro reproductive performance of persistently infected heifers |
| title_sort | effect of bovine viral diarrhea virus on the ovarian functionality and in vitro reproductive performance of persistently infected heifers |
| topic | Novilla Enfermedades de los Animales Pestivirus de la Diarrea Bovina Diarrea Viral Bovina Folículos Ováricos Heifers Animal Diseases Bovine Diarrhoea Pestivirus Bovine Viral Diarrhoea Ovarian Follicles |
| url | https://www.sciencedirect.com/science/article/pii/S0378113513002095 http://hdl.handle.net/20.500.12123/4329 https://doi.org/10.1016/j.vetmic.2013.04.007 |
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