Production of a bovine IL-12p40 probe and application using in situ hybridization on ruminant fixed tissues
Pro-inflammatory cytokines (particularly IL-12) are important for initiating protective T helper 1 (Th1)-type immune responses and hence vital for combating intracellular infections and tumours. In situ hybridization (ISH) provides a powerful diagnostic tool allowing the identification and localizat...
| Autores principales: | , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2019
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| Materias: | |
| Acceso en línea: | https://www.sciencedirect.com/science/article/pii/S0165242712004242 http://hdl.handle.net/20.500.12123/4327 https://doi.org/10.1016/j.vetimm.2012.11.019 |
| _version_ | 1855483411021430784 |
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| author | Canton, German Jose Bartley, Paul M. Bartley, Kathryn Todd, Helen Chianini, Francesca Katzer, Frank |
| author_browse | Bartley, Kathryn Bartley, Paul M. Canton, German Jose Chianini, Francesca Katzer, Frank Todd, Helen |
| author_facet | Canton, German Jose Bartley, Paul M. Bartley, Kathryn Todd, Helen Chianini, Francesca Katzer, Frank |
| author_sort | Canton, German Jose |
| collection | INTA Digital |
| description | Pro-inflammatory cytokines (particularly IL-12) are important for initiating protective T helper 1 (Th1)-type immune responses and hence vital for combating intracellular infections and tumours. In situ hybridization (ISH) provides a powerful diagnostic tool allowing the identification and localization of cells producing these mediators in fixed tissues. The objective of this work was to produce a bovine IL-12p40 probe that allows detection of IL-12p40 mRNA in fixed tissues from different ruminant species. The RNA probe sequence is 447 bp in length and from a region with high cross-species-sequence homology (>97.3% homology) to the ovine, cervine, caprine and bubaline IL-12p40 genes. ISH was carried out on paraformaldehyde fixed tissues collected from cattle, sheep and goats. The probe was efficient in identifying IL-12p40 expressing cells in fixed tissues from all these species. In conclusion, the IL-12p40 probe was efficient in identifying and localizing cells that express IL-12p40, and provides a good immuno-diagnostic technique to characterize immune responses in fixed tissues. |
| format | Artículo |
| id | INTA4327 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA43272019-01-24T14:35:59Z Production of a bovine IL-12p40 probe and application using in situ hybridization on ruminant fixed tissues Canton, German Jose Bartley, Paul M. Bartley, Kathryn Todd, Helen Chianini, Francesca Katzer, Frank Rumiante Citoquinas Hibridación Respuesta Inmunológica Análisis de Tejidos Ruminants Cytokines Hybridization Immune Response Tissue Analysis Pro-inflammatory cytokines (particularly IL-12) are important for initiating protective T helper 1 (Th1)-type immune responses and hence vital for combating intracellular infections and tumours. In situ hybridization (ISH) provides a powerful diagnostic tool allowing the identification and localization of cells producing these mediators in fixed tissues. The objective of this work was to produce a bovine IL-12p40 probe that allows detection of IL-12p40 mRNA in fixed tissues from different ruminant species. The RNA probe sequence is 447 bp in length and from a region with high cross-species-sequence homology (>97.3% homology) to the ovine, cervine, caprine and bubaline IL-12p40 genes. ISH was carried out on paraformaldehyde fixed tissues collected from cattle, sheep and goats. The probe was efficient in identifying IL-12p40 expressing cells in fixed tissues from all these species. In conclusion, the IL-12p40 probe was efficient in identifying and localizing cells that express IL-12p40, and provides a good immuno-diagnostic technique to characterize immune responses in fixed tissues. EEA Balcarce Fil: Canton, German Jose. Moredun Research Institute; Reino Unido. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Bartley, Paul M. Moredun Research Institute; Reino Unido Fil: Bartley, Kathryn. Moredun Research Institute; Reino Unido Fil: Todd, Helen. Moredun Research Institute; Reino Unido Fil: Chianini, Francesca. Moredun Research Institute; Reino Unido Fil: Katzer, Frank. Moredun Research Institute; Reino Unido 2019-01-24T14:34:21Z 2019-01-24T14:34:21Z 2013-02-15 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.sciencedirect.com/science/article/pii/S0165242712004242 http://hdl.handle.net/20.500.12123/4327 0165-2427 1873-2534 https://doi.org/10.1016/j.vetimm.2012.11.019 eng info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Veterinary Immunology and Immunopathology 151 (3–4) : 342-347 (February 2013) |
| spellingShingle | Rumiante Citoquinas Hibridación Respuesta Inmunológica Análisis de Tejidos Ruminants Cytokines Hybridization Immune Response Tissue Analysis Canton, German Jose Bartley, Paul M. Bartley, Kathryn Todd, Helen Chianini, Francesca Katzer, Frank Production of a bovine IL-12p40 probe and application using in situ hybridization on ruminant fixed tissues |
| title | Production of a bovine IL-12p40 probe and application using in situ hybridization on ruminant fixed tissues |
| title_full | Production of a bovine IL-12p40 probe and application using in situ hybridization on ruminant fixed tissues |
| title_fullStr | Production of a bovine IL-12p40 probe and application using in situ hybridization on ruminant fixed tissues |
| title_full_unstemmed | Production of a bovine IL-12p40 probe and application using in situ hybridization on ruminant fixed tissues |
| title_short | Production of a bovine IL-12p40 probe and application using in situ hybridization on ruminant fixed tissues |
| title_sort | production of a bovine il 12p40 probe and application using in situ hybridization on ruminant fixed tissues |
| topic | Rumiante Citoquinas Hibridación Respuesta Inmunológica Análisis de Tejidos Ruminants Cytokines Hybridization Immune Response Tissue Analysis |
| url | https://www.sciencedirect.com/science/article/pii/S0165242712004242 http://hdl.handle.net/20.500.12123/4327 https://doi.org/10.1016/j.vetimm.2012.11.019 |
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