Peanut seed cultivars with contrasting resistance to Aspergillus parasiticus colonization display differential temporal response of protease inhibitors
Significant efforts are being made to minimize aflatoxin contamination in peanut seeds and one possible strategy is to understand and exploit the mechanisms of plant defense against fungal infection. In this study we have identified and characterized, at biochemical and molecular levels, plant prote...
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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American Phytopathological Society
2019
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| Online Access: | https://apsjournals.apsnet.org/doi/pdf/10.1094/PHYTO-09-16-0346-R http://hdl.handle.net/20.500.12123/4322 https://doi.org/10.1094/PHYTO-09-16-0346-R |
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| author | Muller, Virginia Bonacci, Gustavo Roberto Batthyany, Carlos Amé, María Valeria Carrari, Fernando Gieco, Jorge Omar Asis, Ramón |
| author_browse | Amé, María Valeria Asis, Ramón Batthyany, Carlos Bonacci, Gustavo Roberto Carrari, Fernando Gieco, Jorge Omar Muller, Virginia |
| author_facet | Muller, Virginia Bonacci, Gustavo Roberto Batthyany, Carlos Amé, María Valeria Carrari, Fernando Gieco, Jorge Omar Asis, Ramón |
| author_sort | Muller, Virginia |
| collection | INTA Digital |
| description | Significant efforts are being made to minimize aflatoxin contamination in peanut seeds and one possible strategy is to understand and exploit the mechanisms of plant defense against fungal infection. In this study we have identified and characterized, at biochemical and molecular levels, plant protease inhibitors (PPIs) produced in peanut seeds of the resistant PI 337394 and the susceptible Forman cultivar during Aspergillus parasiticus colonization. With chromatographic methods and 2D-electrophoresis-mass spectrometry we have isolated and identified four variants of Bowman-Birk trypsin inhibitor (BBTI) and a novel Kunitz-type protease inhibitor (KPI) produced in response to A. parasiticus colonization. KPI was detected only in the resistant cultivar, while BBTI was produced in the resistant cultivar in a higher concentration than susceptible cultivar and with different isoforms. The kinetic expression of KPI and BBTI genes along with trypsin inhibitory activity was analyzed in both cultivars during infection. In the susceptible cultivar an early PPI activity response was associated with BBTI occurrence. Meanwhile, in the resistant cultivar a later response with a larger increase in PPI activity was associated with BBTI and KPI occurrence. The biological significance of PPI in seed defense against fungal infection was analyzed and linked to inhibitory properties on enzymes released by the fungus during infection, and to the antifungal effect of KPI. |
| format | info:ar-repo/semantics/artículo |
| id | INTA4322 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | American Phytopathological Society |
| publisherStr | American Phytopathological Society |
| record_format | dspace |
| spelling | INTA43222019-01-24T11:54:05Z Peanut seed cultivars with contrasting resistance to Aspergillus parasiticus colonization display differential temporal response of protease inhibitors Muller, Virginia Bonacci, Gustavo Roberto Batthyany, Carlos Amé, María Valeria Carrari, Fernando Gieco, Jorge Omar Asis, Ramón Arachis hypogaea Semilla Variedades Resistencia a la Enfermedad Aspergillus parasiticus Inhibidores de Proteinasas Seeds Varieties Disease Resistance Proteinase Inhibitors Maní Inhibidores de Proteasas Protease Inhibitors Significant efforts are being made to minimize aflatoxin contamination in peanut seeds and one possible strategy is to understand and exploit the mechanisms of plant defense against fungal infection. In this study we have identified and characterized, at biochemical and molecular levels, plant protease inhibitors (PPIs) produced in peanut seeds of the resistant PI 337394 and the susceptible Forman cultivar during Aspergillus parasiticus colonization. With chromatographic methods and 2D-electrophoresis-mass spectrometry we have isolated and identified four variants of Bowman-Birk trypsin inhibitor (BBTI) and a novel Kunitz-type protease inhibitor (KPI) produced in response to A. parasiticus colonization. KPI was detected only in the resistant cultivar, while BBTI was produced in the resistant cultivar in a higher concentration than susceptible cultivar and with different isoforms. The kinetic expression of KPI and BBTI genes along with trypsin inhibitory activity was analyzed in both cultivars during infection. In the susceptible cultivar an early PPI activity response was associated with BBTI occurrence. Meanwhile, in the resistant cultivar a later response with a larger increase in PPI activity was associated with BBTI and KPI occurrence. The biological significance of PPI in seed defense against fungal infection was analyzed and linked to inhibitory properties on enzymes released by the fungus during infection, and to the antifungal effect of KPI. Instituto de Biotecnología Fil: Muller, Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina Fil: Bonacci, Gustavo Roberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina Fil: Batthyany, Carlos. Institut Pasteur de Montevideo. Unidad de Bioquímica y Proteómica Analítica; Uruguay Fil: Amé, María Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina Fil: Carrari, Fernando Oscar. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Gieco, Jorge Omar. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Manfredi; Argentina Fil: Asis, Ramón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina 2019-01-24T11:52:53Z 2019-01-24T11:52:53Z 2017-04 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://apsjournals.apsnet.org/doi/pdf/10.1094/PHYTO-09-16-0346-R http://hdl.handle.net/20.500.12123/4322 0031-949X 1943-7684 https://doi.org/10.1094/PHYTO-09-16-0346-R eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf American Phytopathological Society Phytopathology 107 (4) : 474-482 (April 2017) |
| spellingShingle | Arachis hypogaea Semilla Variedades Resistencia a la Enfermedad Aspergillus parasiticus Inhibidores de Proteinasas Seeds Varieties Disease Resistance Proteinase Inhibitors Maní Inhibidores de Proteasas Protease Inhibitors Muller, Virginia Bonacci, Gustavo Roberto Batthyany, Carlos Amé, María Valeria Carrari, Fernando Gieco, Jorge Omar Asis, Ramón Peanut seed cultivars with contrasting resistance to Aspergillus parasiticus colonization display differential temporal response of protease inhibitors |
| title | Peanut seed cultivars with contrasting resistance to Aspergillus parasiticus colonization display differential temporal response of protease inhibitors |
| title_full | Peanut seed cultivars with contrasting resistance to Aspergillus parasiticus colonization display differential temporal response of protease inhibitors |
| title_fullStr | Peanut seed cultivars with contrasting resistance to Aspergillus parasiticus colonization display differential temporal response of protease inhibitors |
| title_full_unstemmed | Peanut seed cultivars with contrasting resistance to Aspergillus parasiticus colonization display differential temporal response of protease inhibitors |
| title_short | Peanut seed cultivars with contrasting resistance to Aspergillus parasiticus colonization display differential temporal response of protease inhibitors |
| title_sort | peanut seed cultivars with contrasting resistance to aspergillus parasiticus colonization display differential temporal response of protease inhibitors |
| topic | Arachis hypogaea Semilla Variedades Resistencia a la Enfermedad Aspergillus parasiticus Inhibidores de Proteinasas Seeds Varieties Disease Resistance Proteinase Inhibitors Maní Inhibidores de Proteasas Protease Inhibitors |
| url | https://apsjournals.apsnet.org/doi/pdf/10.1094/PHYTO-09-16-0346-R http://hdl.handle.net/20.500.12123/4322 https://doi.org/10.1094/PHYTO-09-16-0346-R |
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