Lipoxygenase activation in peanut seed cultivars resistant and susceptible to Aspergillus parasiticus colonization

Accumulative evidence indicates that the lipoxygenase (LOX) pathway plays a significant role in the Aspergillus–seed interaction, such as interfering with activities of endogenous fungal oxylipins or producing antimicrobial compounds and signaling molecules. In this study, we characterized the LOX p...

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Main Authors: Muller, Virginia, Amé, María Valeria, Carrari, Fernando, Gieco, Jorge Omar, Asis, Ramón
Format: info:ar-repo/semantics/artículo
Language:Inglés
Published: American Phytopathological Society 2019
Subjects:
Online Access:https://apsjournals.apsnet.org/doi/pdf/10.1094/PHYTO-12-13-0338-R
http://hdl.handle.net/20.500.12123/4321
https://doi.org/10.1094/PHYTO-12-13-0338-R
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author Muller, Virginia
Amé, María Valeria
Carrari, Fernando
Gieco, Jorge Omar
Asis, Ramón
author_browse Amé, María Valeria
Asis, Ramón
Carrari, Fernando
Gieco, Jorge Omar
Muller, Virginia
author_facet Muller, Virginia
Amé, María Valeria
Carrari, Fernando
Gieco, Jorge Omar
Asis, Ramón
author_sort Muller, Virginia
collection INTA Digital
description Accumulative evidence indicates that the lipoxygenase (LOX) pathway plays a significant role in the Aspergillus–seed interaction, such as interfering with activities of endogenous fungal oxylipins or producing antimicrobial compounds and signaling molecules. In this study, we characterized the LOX pathway in peanut seed during Aspergillus parasiticus colonization in a model of two cultivars distinguished as resistant (‘PI337394’) and susceptible (‘Florman INTA’) to Aspergillus spp. infection and aflatoxin contamination. The LOX activity together with the content of LOX substrate and LOX products demonstrated the presence of a differential response mechanism to A. parasiticus infection between cultivars. Our findings suggest that this mechanism is under transcriptional control of previously identified (LOX 2 and LOX 3) and novel (LOX 4 and LOX 5) LOX genes. The results of this study support the role of these enzymes in defense during fungus infection in peanut seed.
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institution Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina)
language Inglés
publishDate 2019
publishDateRange 2019
publishDateSort 2019
publisher American Phytopathological Society
publisherStr American Phytopathological Society
record_format dspace
spelling INTA43212019-01-24T11:34:27Z Lipoxygenase activation in peanut seed cultivars resistant and susceptible to Aspergillus parasiticus colonization Muller, Virginia Amé, María Valeria Carrari, Fernando Gieco, Jorge Omar Asis, Ramón Arachis hypogaea Semilla Lipoxigenasa Aspergillus parasiticus Resistencia a la Enfermedad Seeds Lipoxygenase Disease Resistance Maní Accumulative evidence indicates that the lipoxygenase (LOX) pathway plays a significant role in the Aspergillus–seed interaction, such as interfering with activities of endogenous fungal oxylipins or producing antimicrobial compounds and signaling molecules. In this study, we characterized the LOX pathway in peanut seed during Aspergillus parasiticus colonization in a model of two cultivars distinguished as resistant (‘PI337394’) and susceptible (‘Florman INTA’) to Aspergillus spp. infection and aflatoxin contamination. The LOX activity together with the content of LOX substrate and LOX products demonstrated the presence of a differential response mechanism to A. parasiticus infection between cultivars. Our findings suggest that this mechanism is under transcriptional control of previously identified (LOX 2 and LOX 3) and novel (LOX 4 and LOX 5) LOX genes. The results of this study support the role of these enzymes in defense during fungus infection in peanut seed. Instituto de Biotecnología Fil: Muller, Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina Fil: Amé, María Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina Fil: Carrari, Fernando Oscar. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Gieco, Jorge Omar. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Manfredi; Argentina Fil: Asis, Ramón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina 2019-01-24T11:31:21Z 2019-01-24T11:31:21Z 2014-12 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://apsjournals.apsnet.org/doi/pdf/10.1094/PHYTO-12-13-0338-R http://hdl.handle.net/20.500.12123/4321 0031-949X 1943-7684 https://doi.org/10.1094/PHYTO-12-13-0338-R eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf American Phytopathological Society Phytopathology 104 (12) : 1340-1348 (December 2014)
spellingShingle Arachis hypogaea
Semilla
Lipoxigenasa
Aspergillus parasiticus
Resistencia a la Enfermedad
Seeds
Lipoxygenase
Disease Resistance
Maní
Muller, Virginia
Amé, María Valeria
Carrari, Fernando
Gieco, Jorge Omar
Asis, Ramón
Lipoxygenase activation in peanut seed cultivars resistant and susceptible to Aspergillus parasiticus colonization
title Lipoxygenase activation in peanut seed cultivars resistant and susceptible to Aspergillus parasiticus colonization
title_full Lipoxygenase activation in peanut seed cultivars resistant and susceptible to Aspergillus parasiticus colonization
title_fullStr Lipoxygenase activation in peanut seed cultivars resistant and susceptible to Aspergillus parasiticus colonization
title_full_unstemmed Lipoxygenase activation in peanut seed cultivars resistant and susceptible to Aspergillus parasiticus colonization
title_short Lipoxygenase activation in peanut seed cultivars resistant and susceptible to Aspergillus parasiticus colonization
title_sort lipoxygenase activation in peanut seed cultivars resistant and susceptible to aspergillus parasiticus colonization
topic Arachis hypogaea
Semilla
Lipoxigenasa
Aspergillus parasiticus
Resistencia a la Enfermedad
Seeds
Lipoxygenase
Disease Resistance
Maní
url https://apsjournals.apsnet.org/doi/pdf/10.1094/PHYTO-12-13-0338-R
http://hdl.handle.net/20.500.12123/4321
https://doi.org/10.1094/PHYTO-12-13-0338-R
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