Discovery and molecular characterization of a group A rotavirus strain detected in an Argentinean vicuña (Vicugna vicugna)
The wild vicuña (Vicugna vicugna) is one of the four species of native South American camelids (SACs) in addition to the wild guanaco, and their domesticated counterparts, alpaca and llama, respectively. Serological data have indicated the presence of group A rotaviruses (RVA) specific antibodies in...
| Autores principales: | , , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
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Elsevier
2019
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| Materias: | |
| Acceso en línea: | https://www.sciencedirect.com/science/article/pii/S0378113512004208 http://hdl.handle.net/20.500.12123/4318 https://doi.org/10.1016/j.vetmic.2012.07.035 |
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| author | Badaracco, Alejandra Matthijnssens, Jelle Romero, Sandra Raquel Heylen, Elisabeth Zeller, Mark Garaicoechea, Lorena Laura Van Ranst, Marc Parreño, Gladys Viviana |
| author_browse | Badaracco, Alejandra Garaicoechea, Lorena Laura Heylen, Elisabeth Matthijnssens, Jelle Parreño, Gladys Viviana Romero, Sandra Raquel Van Ranst, Marc Zeller, Mark |
| author_facet | Badaracco, Alejandra Matthijnssens, Jelle Romero, Sandra Raquel Heylen, Elisabeth Zeller, Mark Garaicoechea, Lorena Laura Van Ranst, Marc Parreño, Gladys Viviana |
| author_sort | Badaracco, Alejandra |
| collection | INTA Digital |
| description | The wild vicuña (Vicugna vicugna) is one of the four species of native South American camelids (SACs) in addition to the wild guanaco, and their domesticated counterparts, alpaca and llama, respectively. Serological data have indicated the presence of group A rotaviruses (RVA) specific antibodies in all 4 members of the SAC, and so far, RVA has been detected from alpacas, llamas and guanacos. A total of 59 fecal samples from healthy wild newborn and juvenile vicuñas, raised in captivity in Jujuy, Argentina were collected and analyzed by ELISA to detect RVA antigen. Two samples (3%) were found to contain G8 RVA strains and one strain (RVA/Vicuña-wt/ARG/C75/2010/G8P[14]) was selected for further genome analyses, revealing the G8-P[14]-I2-R2-C2-M2-Ax-N2-T6-E3-Hx genotype constellation. Unfortunately, no sequence data could be obtained for NSP1 and NSP5. Except for the E3 NSP4 genotype, this partial genotype constellation is reminiscent to bovine RVA strains and bovine-like RVA strains isolated from sheep, guanaco, antelope and humans. This relationship was confirmed phylogenetically, providing further evidence of the widespread presence of this genotype constellation in animals belonging to the artiodactyls. In particular, a close phylogenetic relationship was found between C75 and guanaco RVA strain RVA/Guanaco-wt/ARG/Chubut/1999/G8P[14] for at least 5 gene segments, suggesting a partial conservation of the genotype constellation of RVA strains infecting different species of SACs, even though nowadays their natural habitats are not overlapping. The further monitoring of the sanitary health of wild newborn and juvenile vicuñas is essential to improve the management practices applied in their sustainable exploitation. |
| format | info:ar-repo/semantics/artículo |
| id | INTA4318 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA43182019-01-23T15:01:13Z Discovery and molecular characterization of a group A rotavirus strain detected in an Argentinean vicuña (Vicugna vicugna) Badaracco, Alejandra Matthijnssens, Jelle Romero, Sandra Raquel Heylen, Elisabeth Zeller, Mark Garaicoechea, Lorena Laura Van Ranst, Marc Parreño, Gladys Viviana Vicuña Virus de los Animales Rotavirus Identificación Anticuerpos Vicunas Animal Viruses Identification Antibodies Argentina The wild vicuña (Vicugna vicugna) is one of the four species of native South American camelids (SACs) in addition to the wild guanaco, and their domesticated counterparts, alpaca and llama, respectively. Serological data have indicated the presence of group A rotaviruses (RVA) specific antibodies in all 4 members of the SAC, and so far, RVA has been detected from alpacas, llamas and guanacos. A total of 59 fecal samples from healthy wild newborn and juvenile vicuñas, raised in captivity in Jujuy, Argentina were collected and analyzed by ELISA to detect RVA antigen. Two samples (3%) were found to contain G8 RVA strains and one strain (RVA/Vicuña-wt/ARG/C75/2010/G8P[14]) was selected for further genome analyses, revealing the G8-P[14]-I2-R2-C2-M2-Ax-N2-T6-E3-Hx genotype constellation. Unfortunately, no sequence data could be obtained for NSP1 and NSP5. Except for the E3 NSP4 genotype, this partial genotype constellation is reminiscent to bovine RVA strains and bovine-like RVA strains isolated from sheep, guanaco, antelope and humans. This relationship was confirmed phylogenetically, providing further evidence of the widespread presence of this genotype constellation in animals belonging to the artiodactyls. In particular, a close phylogenetic relationship was found between C75 and guanaco RVA strain RVA/Guanaco-wt/ARG/Chubut/1999/G8P[14] for at least 5 gene segments, suggesting a partial conservation of the genotype constellation of RVA strains infecting different species of SACs, even though nowadays their natural habitats are not overlapping. The further monitoring of the sanitary health of wild newborn and juvenile vicuñas is essential to improve the management practices applied in their sustainable exploitation. Instituto de Virología Fil: Badaracco, Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Matthijnssens, Jelle. Katholikie Universiteit Leuven. Rega Institute for Medical Research. Laboratory of Clinical & Epidemiological Virology; Bélgica Fil: Romero, Sandra Raquel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Abra Pampa; Argentina Fil: Heylen, Elisabeth. Katholikie Universiteit Leuven. Rega Institute for Medical Research. Laboratory of Clinical & Epidemiological Virology; Bélgica Fil: Zeller, Mark. Katholikie Universiteit Leuven. Rega Institute for Medical Research. Laboratory of Clinical & Epidemiological Virology; Bélgica Fil: Garaicoechea, Lorena Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Van Ranst, Marc. Katholikie Universiteit Leuven. Rega Institute for Medical Research. Laboratory of Clinical & Epidemiological Virology; Bélgica Fil: Parreño, Gladys Viviana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina 2019-01-23T14:59:02Z 2019-01-23T14:59:02Z 2013-01-25 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.sciencedirect.com/science/article/pii/S0378113512004208 http://hdl.handle.net/20.500.12123/4318 0378-1135 https://doi.org/10.1016/j.vetmic.2012.07.035 eng info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Veterinary Microbiology 161 (3–4) : 247-254 (January 2013) |
| spellingShingle | Vicuña Virus de los Animales Rotavirus Identificación Anticuerpos Vicunas Animal Viruses Identification Antibodies Argentina Badaracco, Alejandra Matthijnssens, Jelle Romero, Sandra Raquel Heylen, Elisabeth Zeller, Mark Garaicoechea, Lorena Laura Van Ranst, Marc Parreño, Gladys Viviana Discovery and molecular characterization of a group A rotavirus strain detected in an Argentinean vicuña (Vicugna vicugna) |
| title | Discovery and molecular characterization of a group A rotavirus strain detected in an Argentinean vicuña (Vicugna vicugna) |
| title_full | Discovery and molecular characterization of a group A rotavirus strain detected in an Argentinean vicuña (Vicugna vicugna) |
| title_fullStr | Discovery and molecular characterization of a group A rotavirus strain detected in an Argentinean vicuña (Vicugna vicugna) |
| title_full_unstemmed | Discovery and molecular characterization of a group A rotavirus strain detected in an Argentinean vicuña (Vicugna vicugna) |
| title_short | Discovery and molecular characterization of a group A rotavirus strain detected in an Argentinean vicuña (Vicugna vicugna) |
| title_sort | discovery and molecular characterization of a group a rotavirus strain detected in an argentinean vicuna vicugna vicugna |
| topic | Vicuña Virus de los Animales Rotavirus Identificación Anticuerpos Vicunas Animal Viruses Identification Antibodies Argentina |
| url | https://www.sciencedirect.com/science/article/pii/S0378113512004208 http://hdl.handle.net/20.500.12123/4318 https://doi.org/10.1016/j.vetmic.2012.07.035 |
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