Efficacy of a BVDV subunit vaccine produced in alfalfa transgenic plants
Bovine viral diarrhea virus (BVDV) is considered an important cause of economic loss within bovine herds worldwide. In Argentina, only the use of inactivated vaccines is allowed, however, the efficacy of inactivated BVDV vaccines is variable due to its low immunogenicity. The use of recombinant subu...
| Main Authors: | , , , , , , , , , , |
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| Format: | Artículo |
| Language: | Inglés |
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Elsevier
2019
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| Online Access: | https://www.sciencedirect.com/science/article/pii/S0165242712004291 http://hdl.handle.net/20.500.12123/4317 https://doi.org/10.1016/j.vetimm.2012.12.004 |
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| author | Perez Aguirreburialde, Maria Sol Gomez, Maria Cristina Ostachuck, Agustín Wolman, Federico Javier Albanesi, Guillermo Luis Pecora, Andrea Odeon, Anselmo Carlos Ardila, Fernando Jorge Escribano, José M. Dus Santos, Maria Jose Wigdorovitz, Andres |
| author_browse | Albanesi, Guillermo Luis Ardila, Fernando Jorge Dus Santos, Maria Jose Escribano, José M. Gomez, Maria Cristina Odeon, Anselmo Carlos Ostachuck, Agustín Pecora, Andrea Perez Aguirreburialde, Maria Sol Wigdorovitz, Andres Wolman, Federico Javier |
| author_facet | Perez Aguirreburialde, Maria Sol Gomez, Maria Cristina Ostachuck, Agustín Wolman, Federico Javier Albanesi, Guillermo Luis Pecora, Andrea Odeon, Anselmo Carlos Ardila, Fernando Jorge Escribano, José M. Dus Santos, Maria Jose Wigdorovitz, Andres |
| author_sort | Perez Aguirreburialde, Maria Sol |
| collection | INTA Digital |
| description | Bovine viral diarrhea virus (BVDV) is considered an important cause of economic loss within bovine herds worldwide. In Argentina, only the use of inactivated vaccines is allowed, however, the efficacy of inactivated BVDV vaccines is variable due to its low immunogenicity. The use of recombinant subunit vaccines has been proposed as an alternative to overcome this difficulty. Different studies on protection against BVDV infection have focused the E2 protein, supporting its putative use in subunit vaccines. Utilization of transgenic plants expressing recombinant antigens for the formulation of experimental vaccines represents an innovative and cost effective alternative to the classical fermentation systems.
The aim of this work was to develop transgenic alfalfa plants (Medicago sativa, L.) expressing a truncated version of the structural protein E2 from BVDV fused to a molecule named APCH, that target to antigen presenting cells (APCH-tE2). The concentration of recombinant APCH-tE2 in alfalfa leaves was 1 μg/g at fresh weight and its expression remained stable after vegetative propagation. A methodology based an aqueous two phases system was standardized for concentration and partial purification of APCH-tE2 from alfalfa. Guinea pigs parentally immunized with leaf extracts developed high titers of neutralizing antibodies. In bovine, the APCH-tE2 subunit vaccine was able to induce BVDV-specific neutralizing antibodies. After challenge, bovines inoculated with 3 μg of APCH-tE2 produced in alfalfa transgenic plants showed complete virological protection. |
| format | Artículo |
| id | INTA4317 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA43172019-01-23T14:29:26Z Efficacy of a BVDV subunit vaccine produced in alfalfa transgenic plants Perez Aguirreburialde, Maria Sol Gomez, Maria Cristina Ostachuck, Agustín Wolman, Federico Javier Albanesi, Guillermo Luis Pecora, Andrea Odeon, Anselmo Carlos Ardila, Fernando Jorge Escribano, José M. Dus Santos, Maria Jose Wigdorovitz, Andres Ganado Bovino Enfermedades de los Animales Vacuna Diarrea Viral Bovina Medicago Sativa Plantas Transgénicas Cattle Animal Diseases Vaccines Bovine Viral Diarrhoea Transgenic Plants Alfalfa Bovine viral diarrhea virus (BVDV) is considered an important cause of economic loss within bovine herds worldwide. In Argentina, only the use of inactivated vaccines is allowed, however, the efficacy of inactivated BVDV vaccines is variable due to its low immunogenicity. The use of recombinant subunit vaccines has been proposed as an alternative to overcome this difficulty. Different studies on protection against BVDV infection have focused the E2 protein, supporting its putative use in subunit vaccines. Utilization of transgenic plants expressing recombinant antigens for the formulation of experimental vaccines represents an innovative and cost effective alternative to the classical fermentation systems. The aim of this work was to develop transgenic alfalfa plants (Medicago sativa, L.) expressing a truncated version of the structural protein E2 from BVDV fused to a molecule named APCH, that target to antigen presenting cells (APCH-tE2). The concentration of recombinant APCH-tE2 in alfalfa leaves was 1 μg/g at fresh weight and its expression remained stable after vegetative propagation. A methodology based an aqueous two phases system was standardized for concentration and partial purification of APCH-tE2 from alfalfa. Guinea pigs parentally immunized with leaf extracts developed high titers of neutralizing antibodies. In bovine, the APCH-tE2 subunit vaccine was able to induce BVDV-specific neutralizing antibodies. After challenge, bovines inoculated with 3 μg of APCH-tE2 produced in alfalfa transgenic plants showed complete virological protection. Instituto de Virología Fil: Pérez Aguirreburualde, María Sol. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Gomez, Maria Cristina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Ostachuck, Agustín. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Wolman, Federico Javier. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Fil: Albanesi, Guillermo Luis. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina Fil: Pecora, Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Odeon, Anselmo Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Ardila, Fernando Jorge. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Escribano, José M. Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA). Departamento de Biotecnología; España Fil: Dus Santos, Maria Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Wigdorovitz, Andrés. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina 2019-01-23T14:27:20Z 2019-01-23T14:27:20Z 2013-02-15 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.sciencedirect.com/science/article/pii/S0165242712004291 http://hdl.handle.net/20.500.12123/4317 0165-2427 https://doi.org/10.1016/j.vetimm.2012.12.004 eng info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Veterinary Immunology and Immunopathology 151 (3–4) : 315-324. (February 2013) |
| spellingShingle | Ganado Bovino Enfermedades de los Animales Vacuna Diarrea Viral Bovina Medicago Sativa Plantas Transgénicas Cattle Animal Diseases Vaccines Bovine Viral Diarrhoea Transgenic Plants Alfalfa Perez Aguirreburialde, Maria Sol Gomez, Maria Cristina Ostachuck, Agustín Wolman, Federico Javier Albanesi, Guillermo Luis Pecora, Andrea Odeon, Anselmo Carlos Ardila, Fernando Jorge Escribano, José M. Dus Santos, Maria Jose Wigdorovitz, Andres Efficacy of a BVDV subunit vaccine produced in alfalfa transgenic plants |
| title | Efficacy of a BVDV subunit vaccine produced in alfalfa transgenic plants |
| title_full | Efficacy of a BVDV subunit vaccine produced in alfalfa transgenic plants |
| title_fullStr | Efficacy of a BVDV subunit vaccine produced in alfalfa transgenic plants |
| title_full_unstemmed | Efficacy of a BVDV subunit vaccine produced in alfalfa transgenic plants |
| title_short | Efficacy of a BVDV subunit vaccine produced in alfalfa transgenic plants |
| title_sort | efficacy of a bvdv subunit vaccine produced in alfalfa transgenic plants |
| topic | Ganado Bovino Enfermedades de los Animales Vacuna Diarrea Viral Bovina Medicago Sativa Plantas Transgénicas Cattle Animal Diseases Vaccines Bovine Viral Diarrhoea Transgenic Plants Alfalfa |
| url | https://www.sciencedirect.com/science/article/pii/S0165242712004291 http://hdl.handle.net/20.500.12123/4317 https://doi.org/10.1016/j.vetimm.2012.12.004 |
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