Polymorphisms at the 3′ untranslated region of SLC11A1 gene are associated with protection to Brucella infection in goats
Goats are susceptible to brucellosis and the detection of Brucella-infected animals is carried out by serological tests. In other ruminant species, polymorphisms in microsatellites (Ms) of 3′ untranslated region (3′UTR) of the solute carrier family 11 member A1 (SLC11A1) gene were associated with re...
| Autores principales: | , , , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2019
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| Acceso en línea: | https://www.sciencedirect.com/science/article/pii/S0165242714001202 http://hdl.handle.net/20.500.12123/4315 https://doi.org/10.1016/j.vetimm.2014.05.007 |
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| author | Iacoboni, Paola Anabella Hasenauer, Flavia Carolina Caffaro, María Eugenia Gaido, Analia Beatriz Rossetto, Cristina Neumann, Roberto Daniel Salatin, Antonio Omar Bertoni, Emiliano Agustín Poli, Mario Andres Rossetti, Carlos Alberto |
| author_browse | Bertoni, Emiliano Agustín Caffaro, María Eugenia Gaido, Analia Beatriz Hasenauer, Flavia Carolina Iacoboni, Paola Anabella Neumann, Roberto Daniel Poli, Mario Andres Rossetti, Carlos Alberto Rossetto, Cristina Salatin, Antonio Omar |
| author_facet | Iacoboni, Paola Anabella Hasenauer, Flavia Carolina Caffaro, María Eugenia Gaido, Analia Beatriz Rossetto, Cristina Neumann, Roberto Daniel Salatin, Antonio Omar Bertoni, Emiliano Agustín Poli, Mario Andres Rossetti, Carlos Alberto |
| author_sort | Iacoboni, Paola Anabella |
| collection | INTA Digital |
| description | Goats are susceptible to brucellosis and the detection of Brucella-infected animals is carried out by serological tests. In other ruminant species, polymorphisms in microsatellites (Ms) of 3′ untranslated region (3′UTR) of the solute carrier family 11 member A1 (SLC11A1) gene were associated with resistance to Brucella abortus infection. Goats present two polymorphic Ms at the 3′UTR end of SLC11A1 gene, called regions A and B. Here, we evaluated if polymorphisms in regions A and/or B are associated with Brucella infection in goats. Serum (for the detection of Brucella-specific antibodies) and hair samples (for DNA isolation and structure analysis of the SLC11A1 gene) were randomly collected from 229 adult native goats from the northwest of Argentina. Serological status was evaluated by buffer plate antigen test (BPAT) complemented by the fluorescent polarization assay (FPA), and the genotype of the 3′UTR of the SLC11A1 gene was determined by capillary electrophoresis and confirmed by sequence analysis. Polymorphisms in regions A and B of the 3′UTR SLC11A1 gene were found statistically significant associated with protection to Brucella infection. Specifically, the association study indicates statistical significance of the allele A15 and B7/B7 genotype with absence of Brucella-specific antibodies (p = 0.0003 and 0.0088, respectively). These data open a promising opportunity for limiting goat brucellosis through selective breeding of animals based on genetic markers associated with natural resistance to B. melitensis infection. |
| format | Artículo |
| id | INTA4315 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA43152019-01-23T13:03:33Z Polymorphisms at the 3′ untranslated region of SLC11A1 gene are associated with protection to Brucella infection in goats Iacoboni, Paola Anabella Hasenauer, Flavia Carolina Caffaro, María Eugenia Gaido, Analia Beatriz Rossetto, Cristina Neumann, Roberto Daniel Salatin, Antonio Omar Bertoni, Emiliano Agustín Poli, Mario Andres Rossetti, Carlos Alberto Caprinos Genética Polimorfismo Brucella Brucelosis Goats Genetics Polymorphism Brucellosis Goats are susceptible to brucellosis and the detection of Brucella-infected animals is carried out by serological tests. In other ruminant species, polymorphisms in microsatellites (Ms) of 3′ untranslated region (3′UTR) of the solute carrier family 11 member A1 (SLC11A1) gene were associated with resistance to Brucella abortus infection. Goats present two polymorphic Ms at the 3′UTR end of SLC11A1 gene, called regions A and B. Here, we evaluated if polymorphisms in regions A and/or B are associated with Brucella infection in goats. Serum (for the detection of Brucella-specific antibodies) and hair samples (for DNA isolation and structure analysis of the SLC11A1 gene) were randomly collected from 229 adult native goats from the northwest of Argentina. Serological status was evaluated by buffer plate antigen test (BPAT) complemented by the fluorescent polarization assay (FPA), and the genotype of the 3′UTR of the SLC11A1 gene was determined by capillary electrophoresis and confirmed by sequence analysis. Polymorphisms in regions A and B of the 3′UTR SLC11A1 gene were found statistically significant associated with protection to Brucella infection. Specifically, the association study indicates statistical significance of the allele A15 and B7/B7 genotype with absence of Brucella-specific antibodies (p = 0.0003 and 0.0088, respectively). These data open a promising opportunity for limiting goat brucellosis through selective breeding of animals based on genetic markers associated with natural resistance to B. melitensis infection. Instituto de Patobiología Fil: Iacoboni, Paola Anabella. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina Fil: Hasenauer, Flavia Carolina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Caffaro, María Eugenia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Gaido, Analía Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina Fil: Rossetto, Cristina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Yuto; Argentina Fil: Neumann, Roberto Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina Fil: Salatin, Antonio Omar. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina Fil: Bertoni, Emiliano Agustín. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina Fil: Poli, Mario Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Rossetti, Carlos Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina 2019-01-23T13:01:40Z 2019-01-23T13:01:40Z 2014-08-15 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.sciencedirect.com/science/article/pii/S0165242714001202 http://hdl.handle.net/20.500.12123/4315 0165-2427 https://doi.org/10.1016/j.vetimm.2014.05.007 eng info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Veterinary Immunology and Immunopathology 160 (3–4) : 230-234 (August 2014) |
| spellingShingle | Caprinos Genética Polimorfismo Brucella Brucelosis Goats Genetics Polymorphism Brucellosis Iacoboni, Paola Anabella Hasenauer, Flavia Carolina Caffaro, María Eugenia Gaido, Analia Beatriz Rossetto, Cristina Neumann, Roberto Daniel Salatin, Antonio Omar Bertoni, Emiliano Agustín Poli, Mario Andres Rossetti, Carlos Alberto Polymorphisms at the 3′ untranslated region of SLC11A1 gene are associated with protection to Brucella infection in goats |
| title | Polymorphisms at the 3′ untranslated region of SLC11A1 gene are associated with protection to Brucella infection in goats |
| title_full | Polymorphisms at the 3′ untranslated region of SLC11A1 gene are associated with protection to Brucella infection in goats |
| title_fullStr | Polymorphisms at the 3′ untranslated region of SLC11A1 gene are associated with protection to Brucella infection in goats |
| title_full_unstemmed | Polymorphisms at the 3′ untranslated region of SLC11A1 gene are associated with protection to Brucella infection in goats |
| title_short | Polymorphisms at the 3′ untranslated region of SLC11A1 gene are associated with protection to Brucella infection in goats |
| title_sort | polymorphisms at the 3 untranslated region of slc11a1 gene are associated with protection to brucella infection in goats |
| topic | Caprinos Genética Polimorfismo Brucella Brucelosis Goats Genetics Polymorphism Brucellosis |
| url | https://www.sciencedirect.com/science/article/pii/S0165242714001202 http://hdl.handle.net/20.500.12123/4315 https://doi.org/10.1016/j.vetimm.2014.05.007 |
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