Physiopathological effects of Escherichia coli O157:H7 inoculation in weaned calves fed with colostrum containing antibodies to EspB and Intimin
Escherichia coli O157:H7 is responsible for severe intestinal disease and hemolytic uremic syndrome (HUS), a serious systemic complication which particularly affects children. Cattle are the primary reservoir for E. coli O157:H7 and the main source of infection for humans. In this study, we evaluate...
| Autores principales: | , , , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
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Elsevier
2019
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| Materias: | |
| Acceso en línea: | https://www.sciencedirect.com/science/article/pii/S0264410X14006112 http://hdl.handle.net/20.500.12123/4314 https://doi.org/10.1016/j.vaccine.2014.04.073 |
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| author | Rabinovitz, Bettina Carol Vilte, Daniel Alejandro Larzabal, Mariano Abdala, Alejandro Ariel Galarza, Roxana Ivon Zotta, Elsa Ibarra, Cristina E. Mercado, Elsa Cristina Cataldi, Angel Adrian |
| author_browse | Abdala, Alejandro Ariel Cataldi, Angel Adrian Galarza, Roxana Ivon Ibarra, Cristina E. Larzabal, Mariano Mercado, Elsa Cristina Rabinovitz, Bettina Carol Vilte, Daniel Alejandro Zotta, Elsa |
| author_facet | Rabinovitz, Bettina Carol Vilte, Daniel Alejandro Larzabal, Mariano Abdala, Alejandro Ariel Galarza, Roxana Ivon Zotta, Elsa Ibarra, Cristina E. Mercado, Elsa Cristina Cataldi, Angel Adrian |
| author_sort | Rabinovitz, Bettina Carol |
| collection | INTA Digital |
| description | Escherichia coli O157:H7 is responsible for severe intestinal disease and hemolytic uremic syndrome (HUS), a serious systemic complication which particularly affects children. Cattle are the primary reservoir for E. coli O157:H7 and the main source of infection for humans. In this study, we evaluated the ability of transferred maternal colostral antibodies against γ-Intimin C280 and EspB, to protect young weaned calves from E. coli O157:H7 infection. Hyperimmune colostra were obtained by immunization of pregnant cows with a mix of the mentioned antigens. All vaccinated cows mounted a significant IgG response against γ-Intimin C280, and EspB in sera and colostra. Colostrum-fed calves also exhibited high serum IgG titers against γ-Intimin C280 and EspB along with a rise in mucosal γ-Intimin C280-specific IgG antibodies at recto-anal junction and ileum. Additionally, 70 day-old calves received a challenge with E. coli O157:H7 but no reduction in total bacterial shedding or frequency of E. coli O157:H7 excretion from these calves was observed. Most tissue samples showed granulocyte focal infiltrations of the lamina propria and enterocyte erosion. In conclusion, up to the 70th day, the passively acquired γ-Intimin-C280 and EspB-IgG antibodies present in sera and recto-anal mucosa reached a titer insufficient to reduce EHEC O157 shedding and damages of experimentally inoculated young calves. |
| format | info:ar-repo/semantics/artículo |
| id | INTA4314 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA43142019-01-23T12:17:44Z Physiopathological effects of Escherichia coli O157:H7 inoculation in weaned calves fed with colostrum containing antibodies to EspB and Intimin Rabinovitz, Bettina Carol Vilte, Daniel Alejandro Larzabal, Mariano Abdala, Alejandro Ariel Galarza, Roxana Ivon Zotta, Elsa Ibarra, Cristina E. Mercado, Elsa Cristina Cataldi, Angel Adrian Escherichia coli Inoculación Ternero Alimentación de los Animales Calostro Anticuerpos Destete Inoculation Calves Animal Feeding Colostrum Antibodies Weaning Escherichia coli O157:H7 Escherichia coli O157:H7 is responsible for severe intestinal disease and hemolytic uremic syndrome (HUS), a serious systemic complication which particularly affects children. Cattle are the primary reservoir for E. coli O157:H7 and the main source of infection for humans. In this study, we evaluated the ability of transferred maternal colostral antibodies against γ-Intimin C280 and EspB, to protect young weaned calves from E. coli O157:H7 infection. Hyperimmune colostra were obtained by immunization of pregnant cows with a mix of the mentioned antigens. All vaccinated cows mounted a significant IgG response against γ-Intimin C280, and EspB in sera and colostra. Colostrum-fed calves also exhibited high serum IgG titers against γ-Intimin C280 and EspB along with a rise in mucosal γ-Intimin C280-specific IgG antibodies at recto-anal junction and ileum. Additionally, 70 day-old calves received a challenge with E. coli O157:H7 but no reduction in total bacterial shedding or frequency of E. coli O157:H7 excretion from these calves was observed. Most tissue samples showed granulocyte focal infiltrations of the lamina propria and enterocyte erosion. In conclusion, up to the 70th day, the passively acquired γ-Intimin-C280 and EspB-IgG antibodies present in sera and recto-anal mucosa reached a titer insufficient to reduce EHEC O157 shedding and damages of experimentally inoculated young calves. Instituto de Patobiología Fil: Rabinovitz, Bettina Carol. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina Fil: Vilte, Daniel Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina Fil: Larzabal, Mariano. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Abdala, Alejandro Ariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina Fil: Galarza, Roxana Ivon. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina Fil: Zotta, Elsa. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Fisiología. Laboratorio de Fisiopatogenia; Argentina Fil: Ibarra, Cristina E. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Fisiología. Laboratorio de Fisiopatogenia; Argentina Fil: Mercado, Elsa Cristina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina Fil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina 2019-01-23T12:16:07Z 2019-01-23T12:16:07Z 2014-06-24 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.sciencedirect.com/science/article/pii/S0264410X14006112 http://hdl.handle.net/20.500.12123/4314 0264-410X https://doi.org/10.1016/j.vaccine.2014.04.073 eng info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Vaccine 32 (30) : 3823-3829 (June 2014) |
| spellingShingle | Escherichia coli Inoculación Ternero Alimentación de los Animales Calostro Anticuerpos Destete Inoculation Calves Animal Feeding Colostrum Antibodies Weaning Escherichia coli O157:H7 Rabinovitz, Bettina Carol Vilte, Daniel Alejandro Larzabal, Mariano Abdala, Alejandro Ariel Galarza, Roxana Ivon Zotta, Elsa Ibarra, Cristina E. Mercado, Elsa Cristina Cataldi, Angel Adrian Physiopathological effects of Escherichia coli O157:H7 inoculation in weaned calves fed with colostrum containing antibodies to EspB and Intimin |
| title | Physiopathological effects of Escherichia coli O157:H7 inoculation in weaned calves fed with colostrum containing antibodies to EspB and Intimin |
| title_full | Physiopathological effects of Escherichia coli O157:H7 inoculation in weaned calves fed with colostrum containing antibodies to EspB and Intimin |
| title_fullStr | Physiopathological effects of Escherichia coli O157:H7 inoculation in weaned calves fed with colostrum containing antibodies to EspB and Intimin |
| title_full_unstemmed | Physiopathological effects of Escherichia coli O157:H7 inoculation in weaned calves fed with colostrum containing antibodies to EspB and Intimin |
| title_short | Physiopathological effects of Escherichia coli O157:H7 inoculation in weaned calves fed with colostrum containing antibodies to EspB and Intimin |
| title_sort | physiopathological effects of escherichia coli o157 h7 inoculation in weaned calves fed with colostrum containing antibodies to espb and intimin |
| topic | Escherichia coli Inoculación Ternero Alimentación de los Animales Calostro Anticuerpos Destete Inoculation Calves Animal Feeding Colostrum Antibodies Weaning Escherichia coli O157:H7 |
| url | https://www.sciencedirect.com/science/article/pii/S0264410X14006112 http://hdl.handle.net/20.500.12123/4314 https://doi.org/10.1016/j.vaccine.2014.04.073 |
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