Co-inoculation of baculovirus and FMDV vaccine in mice, elicits very early protection against foot and mouth disease virus without interfering with long lasting immunity

Baculoviruses (Bvs) potentiate the immune response against soluble antigens. We investigated whether Bv could be used as immunoactivator in foot-and-mouth disease (FMD) vaccines using the BALB/c mouse model. Mice were vaccinated with a single dose of inactivated FMDV (iFMDV), iFMDV + Bv, Bv, or cult...

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Autores principales: Quattrocchi, Valeria, Molinari, Maria Paula, Langellotti, Cecilia Ana, Gnazzo, Victoria, Taboga, Oscar Alberto, Zamorano, Patricia Ines
Formato: info:ar-repo/semantics/artículo
Lenguaje:Inglés
Publicado: Elsevier 2019
Materias:
Acceso en línea:https://www.sciencedirect.com/science/article/pii/S0264410X13004131
http://hdl.handle.net/20.500.12123/4312
https://doi.org/10.1016/j.vaccine.2013.03.067
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author Quattrocchi, Valeria
Molinari, Maria Paula
Langellotti, Cecilia Ana
Gnazzo, Victoria
Taboga, Oscar Alberto
Zamorano, Patricia Ines
author_browse Gnazzo, Victoria
Langellotti, Cecilia Ana
Molinari, Maria Paula
Quattrocchi, Valeria
Taboga, Oscar Alberto
Zamorano, Patricia Ines
author_facet Quattrocchi, Valeria
Molinari, Maria Paula
Langellotti, Cecilia Ana
Gnazzo, Victoria
Taboga, Oscar Alberto
Zamorano, Patricia Ines
author_sort Quattrocchi, Valeria
collection INTA Digital
description Baculoviruses (Bvs) potentiate the immune response against soluble antigens. We investigated whether Bv could be used as immunoactivator in foot-and-mouth disease (FMD) vaccines using the BALB/c mouse model. Mice were vaccinated with a single dose of inactivated FMDV (iFMDV), iFMDV + Bv, Bv, or culture medium. Humoral and cellular immune responses were higher in animals immunized with iFMDV + Bv than in mice vaccinated with iFMDV alone. Animals receiving iFMDV + Bv had significantly lower viremia at 2, 4 and 7 dpv, than those immunized with iFMDV alone. In order to prolong the immune response, iFMDV oil vaccine was co-inoculated with Bv. Animals receiving iFMDV oil vaccine + Bv were protected two days earlier than those receiving the iFMDV oil vaccine alone. Both formulations protected until 14 dpv, the last day of the experiment. This is the first report in which Bv is used as an adjuvant in a FMDV vaccine.
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institution Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina)
language Inglés
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publishDateRange 2019
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spelling INTA43122019-02-19T13:58:44Z Co-inoculation of baculovirus and FMDV vaccine in mice, elicits very early protection against foot and mouth disease virus without interfering with long lasting immunity Quattrocchi, Valeria Molinari, Maria Paula Langellotti, Cecilia Ana Gnazzo, Victoria Taboga, Oscar Alberto Zamorano, Patricia Ines Virus Fiebre Aftosa Ratón Baculovirus Inoculación Vacuna Inmunidad Enfermedades de los Animales Aphthovirus Mice Inoculation Vaccines Immunity Animal Diseases Baculoviruses (Bvs) potentiate the immune response against soluble antigens. We investigated whether Bv could be used as immunoactivator in foot-and-mouth disease (FMD) vaccines using the BALB/c mouse model. Mice were vaccinated with a single dose of inactivated FMDV (iFMDV), iFMDV + Bv, Bv, or culture medium. Humoral and cellular immune responses were higher in animals immunized with iFMDV + Bv than in mice vaccinated with iFMDV alone. Animals receiving iFMDV + Bv had significantly lower viremia at 2, 4 and 7 dpv, than those immunized with iFMDV alone. In order to prolong the immune response, iFMDV oil vaccine was co-inoculated with Bv. Animals receiving iFMDV oil vaccine + Bv were protected two days earlier than those receiving the iFMDV oil vaccine alone. Both formulations protected until 14 dpv, the last day of the experiment. This is the first report in which Bv is used as an adjuvant in a FMDV vaccine. Instituto de Virología Fil: Quattrocchi, Valeria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Molinari, Maria Paula. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Langellotti, Cecilia Ana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Gnazzo, Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Taboga, Oscar Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Zamorano, Patricia Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad del Salvador; Argentina 2019-01-22T15:42:12Z 2019-01-22T15:42:12Z 2013-05-31 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.sciencedirect.com/science/article/pii/S0264410X13004131 http://hdl.handle.net/20.500.12123/4312 0264-410X https://doi.org/10.1016/j.vaccine.2013.03.067 eng info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Vaccine 31 (24) : 2713-2718 (May 2013)
spellingShingle Virus Fiebre Aftosa
Ratón
Baculovirus
Inoculación
Vacuna
Inmunidad
Enfermedades de los Animales
Aphthovirus
Mice
Inoculation
Vaccines
Immunity
Animal Diseases
Quattrocchi, Valeria
Molinari, Maria Paula
Langellotti, Cecilia Ana
Gnazzo, Victoria
Taboga, Oscar Alberto
Zamorano, Patricia Ines
Co-inoculation of baculovirus and FMDV vaccine in mice, elicits very early protection against foot and mouth disease virus without interfering with long lasting immunity
title Co-inoculation of baculovirus and FMDV vaccine in mice, elicits very early protection against foot and mouth disease virus without interfering with long lasting immunity
title_full Co-inoculation of baculovirus and FMDV vaccine in mice, elicits very early protection against foot and mouth disease virus without interfering with long lasting immunity
title_fullStr Co-inoculation of baculovirus and FMDV vaccine in mice, elicits very early protection against foot and mouth disease virus without interfering with long lasting immunity
title_full_unstemmed Co-inoculation of baculovirus and FMDV vaccine in mice, elicits very early protection against foot and mouth disease virus without interfering with long lasting immunity
title_short Co-inoculation of baculovirus and FMDV vaccine in mice, elicits very early protection against foot and mouth disease virus without interfering with long lasting immunity
title_sort co inoculation of baculovirus and fmdv vaccine in mice elicits very early protection against foot and mouth disease virus without interfering with long lasting immunity
topic Virus Fiebre Aftosa
Ratón
Baculovirus
Inoculación
Vacuna
Inmunidad
Enfermedades de los Animales
Aphthovirus
Mice
Inoculation
Vaccines
Immunity
Animal Diseases
url https://www.sciencedirect.com/science/article/pii/S0264410X13004131
http://hdl.handle.net/20.500.12123/4312
https://doi.org/10.1016/j.vaccine.2013.03.067
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