Experimental inoculation of Neospora caninum in pregnant water buffalo
The aim of this study was to characterize the pathogenesis of Neospora caninum in experimentally inoculated pregnant water buffalo (Bubalus bubalis). Twelve Mediterranean female water buffaloes ranging in age from 4 to 14 years old and seronegative to N. caninum by indirect fluorescent antibody test...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Artículo |
| Language: | Inglés |
| Published: |
Elsevier
2019
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| Online Access: | http://hdl.handle.net/20.500.12123/4307 https://www.sciencedirect.com/science/article/pii/S0304401711008685 https://doi.org/10.1016/j.vetpar.2011.12.030 |
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| author | Konrad, Jose Luis Moore, Prando Dadin Crudeli, Gustavo Angel Caspe, Sergio Gaston Cano, Dora Beatriz Leunda, Maria Rosa Lischinsky, Lilian Haydee Regidor-Cerrillo, Javier Odeon, Anselmo Carlos Ortega-Mora, Luis M. Echaide, Ignacio Eduardo Campero, Carlos Manuel |
| author_browse | Campero, Carlos Manuel Cano, Dora Beatriz Caspe, Sergio Gaston Crudeli, Gustavo Angel Echaide, Ignacio Eduardo Konrad, Jose Luis Leunda, Maria Rosa Lischinsky, Lilian Haydee Moore, Prando Dadin Odeon, Anselmo Carlos Ortega-Mora, Luis M. Regidor-Cerrillo, Javier |
| author_facet | Konrad, Jose Luis Moore, Prando Dadin Crudeli, Gustavo Angel Caspe, Sergio Gaston Cano, Dora Beatriz Leunda, Maria Rosa Lischinsky, Lilian Haydee Regidor-Cerrillo, Javier Odeon, Anselmo Carlos Ortega-Mora, Luis M. Echaide, Ignacio Eduardo Campero, Carlos Manuel |
| author_sort | Konrad, Jose Luis |
| collection | INTA Digital |
| description | The aim of this study was to characterize the pathogenesis of Neospora caninum in experimentally inoculated pregnant water buffalo (Bubalus bubalis). Twelve Mediterranean female water buffaloes ranging in age from 4 to 14 years old and seronegative to N. caninum by indirect fluorescent antibody test (IFAT) were involved. Ten females were intravenously inoculated with 108 tachyzoites of NC-1 strain at 70 (n = 3) or 90 (n = 7) days of pregnancy (dp). Two control animals were inoculated with placebo at 70 and 90 dp, respectively. Serum samples were obtained weekly following inoculation to the end of the experiment. Three animals inoculated at 70 dp were slaughtered at 28 days post inoculation (dpi), three animals inoculated at 90 dp were slaughtered at 28 dpi and the remaining four animals inoculated at 90 dp were slaughtered at 42 dpi. Fetal fluids from cavities and tissue samples were recovered for IFAT and histopathology, immunohistochemistry and PCR, respectively. Genomic DNA from fetal tissues was used for parasite DNA detection and microsatellite genotyping in order to confirm the NC-1 specific-infection. Dams developed specific antibodies one week after the inoculation and serological titers did not decrease significantly to the end of the experiment. No abortions were recorded during the experimental time; however, one fetus from a dam inoculated at 70 dp was not viable at necropsy. Specific antibodies were detected in only two fetuses from dams inoculated at 90 dp that were slaughtered at 42 dpi. No macroscopic changes in the placentas and organs of viable fetuses were observed. Nonsuppurative placentitis was a common microscopic observation in Neospora-inoculated specimens. Microscopic fetal lesions included nonsuppurative peribronchiolar interstitial pneumonia, epicarditis and myocarditis, interstitial nephritis, myositis and periportal hepatitis. Positive IHC results were obtained in two fetuses from dams inoculated at 70 dp and slaughtered at 28 dpi. N. caninum DNA was detected in placentas and fetuses from all inoculated animals. The pattern of amplified microsatellites from placental and fetal tissues resembled the NC-1 strain. Water buffaloes, like cattle, are susceptible to experimental inoculation with N. caninum at early pregnancy. |
| format | Artículo |
| id | INTA4307 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA43072021-09-30T13:41:43Z Experimental inoculation of Neospora caninum in pregnant water buffalo Konrad, Jose Luis Moore, Prando Dadin Crudeli, Gustavo Angel Caspe, Sergio Gaston Cano, Dora Beatriz Leunda, Maria Rosa Lischinsky, Lilian Haydee Regidor-Cerrillo, Javier Odeon, Anselmo Carlos Ortega-Mora, Luis M. Echaide, Ignacio Eduardo Campero, Carlos Manuel Búfalo de Agua Enfermedades de los Animales Neospora caninum Infección Experimental Gestación Water Buffaloes Animal Diseases Experimental Infection Pregnancy The aim of this study was to characterize the pathogenesis of Neospora caninum in experimentally inoculated pregnant water buffalo (Bubalus bubalis). Twelve Mediterranean female water buffaloes ranging in age from 4 to 14 years old and seronegative to N. caninum by indirect fluorescent antibody test (IFAT) were involved. Ten females were intravenously inoculated with 108 tachyzoites of NC-1 strain at 70 (n = 3) or 90 (n = 7) days of pregnancy (dp). Two control animals were inoculated with placebo at 70 and 90 dp, respectively. Serum samples were obtained weekly following inoculation to the end of the experiment. Three animals inoculated at 70 dp were slaughtered at 28 days post inoculation (dpi), three animals inoculated at 90 dp were slaughtered at 28 dpi and the remaining four animals inoculated at 90 dp were slaughtered at 42 dpi. Fetal fluids from cavities and tissue samples were recovered for IFAT and histopathology, immunohistochemistry and PCR, respectively. Genomic DNA from fetal tissues was used for parasite DNA detection and microsatellite genotyping in order to confirm the NC-1 specific-infection. Dams developed specific antibodies one week after the inoculation and serological titers did not decrease significantly to the end of the experiment. No abortions were recorded during the experimental time; however, one fetus from a dam inoculated at 70 dp was not viable at necropsy. Specific antibodies were detected in only two fetuses from dams inoculated at 90 dp that were slaughtered at 42 dpi. No macroscopic changes in the placentas and organs of viable fetuses were observed. Nonsuppurative placentitis was a common microscopic observation in Neospora-inoculated specimens. Microscopic fetal lesions included nonsuppurative peribronchiolar interstitial pneumonia, epicarditis and myocarditis, interstitial nephritis, myositis and periportal hepatitis. Positive IHC results were obtained in two fetuses from dams inoculated at 70 dp and slaughtered at 28 dpi. N. caninum DNA was detected in placentas and fetuses from all inoculated animals. The pattern of amplified microsatellites from placental and fetal tissues resembled the NC-1 strain. Water buffaloes, like cattle, are susceptible to experimental inoculation with N. caninum at early pregnancy. EEA Mercedes Fil: Konrad, Jose Luis. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias. Cátedra de Teriogenología; Argentina Fil: Moore, Prando Dadin. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Crudeli, Gustavo Angel. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias. Cátedra de Teriogenología; Argentina Fil: Caspe, Sergio Gaston. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mercedes; Argentina Fil: Cano, Dora Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Leunda, Maria Rosa. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Lischinsky, Lilian Haydee. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Regidor-Cerrillo, Javier. Universidad Complutense de Madrid. Facultad de Ciencias Veterinarias. Departamento de Salud Animal. SALUVET; España Fil: Odeon, Anselmo Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Ortega-Mora, Luis M. Universidad Complutense de Madrid. Facultad de Ciencias Veterinarias. Departamento de Salud Animal. SALUVET; España Fil: Echaide, Ignacio Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina Fil: Campero, Carlos Manuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina 2019-01-22T12:17:52Z 2019-01-22T12:17:52Z 2012-06-08 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/4307 https://www.sciencedirect.com/science/article/pii/S0304401711008685 0304-4017 https://doi.org/10.1016/j.vetpar.2011.12.030 eng info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Veterinary Parasitology 187 (1–2) : 72-78 (June 2012) |
| spellingShingle | Búfalo de Agua Enfermedades de los Animales Neospora caninum Infección Experimental Gestación Water Buffaloes Animal Diseases Experimental Infection Pregnancy Konrad, Jose Luis Moore, Prando Dadin Crudeli, Gustavo Angel Caspe, Sergio Gaston Cano, Dora Beatriz Leunda, Maria Rosa Lischinsky, Lilian Haydee Regidor-Cerrillo, Javier Odeon, Anselmo Carlos Ortega-Mora, Luis M. Echaide, Ignacio Eduardo Campero, Carlos Manuel Experimental inoculation of Neospora caninum in pregnant water buffalo |
| title | Experimental inoculation of Neospora caninum in pregnant water buffalo |
| title_full | Experimental inoculation of Neospora caninum in pregnant water buffalo |
| title_fullStr | Experimental inoculation of Neospora caninum in pregnant water buffalo |
| title_full_unstemmed | Experimental inoculation of Neospora caninum in pregnant water buffalo |
| title_short | Experimental inoculation of Neospora caninum in pregnant water buffalo |
| title_sort | experimental inoculation of neospora caninum in pregnant water buffalo |
| topic | Búfalo de Agua Enfermedades de los Animales Neospora caninum Infección Experimental Gestación Water Buffaloes Animal Diseases Experimental Infection Pregnancy |
| url | http://hdl.handle.net/20.500.12123/4307 https://www.sciencedirect.com/science/article/pii/S0304401711008685 https://doi.org/10.1016/j.vetpar.2011.12.030 |
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