Long-term effect of tillage systems on soil microbiological, chemical and physical parameters and the incidence of charcoal rot by Macrophomina phaseolina (Tassi) Goid in soybean
A 20-year field experiment was employed with the aim of evaluating the effect of tillage systems on biological, chemical and physical aspects of the soil, and to establish whether there was a correlation of these parameters with the incidence of charcoal rot (Macrophomina phaseolina) of soybean and...
| Main Authors: | , , , , , , , , |
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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Elsevier
2019
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| Online Access: | https://www.sciencedirect.com/science/article/pii/S0261219412001159 http://hdl.handle.net/20.500.12123/4302 https://doi.org/10.1016/j.cropro.2012.04.018 |
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| author | Perez Brandan, Carolina Arzeno, Jose Luis Huidobro, Dina Jorgelina Grumberg, Betiana Clarisa Conforto, Erica Cinthia Hilton, Sally Bending, Gary D. Meriles, Jose Manuel Vargas Gil, Silvina |
| author_browse | Arzeno, Jose Luis Bending, Gary D. Conforto, Erica Cinthia Grumberg, Betiana Clarisa Hilton, Sally Huidobro, Dina Jorgelina Meriles, Jose Manuel Perez Brandan, Carolina Vargas Gil, Silvina |
| author_facet | Perez Brandan, Carolina Arzeno, Jose Luis Huidobro, Dina Jorgelina Grumberg, Betiana Clarisa Conforto, Erica Cinthia Hilton, Sally Bending, Gary D. Meriles, Jose Manuel Vargas Gil, Silvina |
| author_sort | Perez Brandan, Carolina |
| collection | INTA Digital |
| description | A 20-year field experiment was employed with the aim of evaluating the effect of tillage systems on biological, chemical and physical aspects of the soil, and to establish whether there was a correlation of these parameters with the incidence of charcoal rot (Macrophomina phaseolina) of soybean and crop yield. The tillage systems evaluated were direct seeding (DS), DS + scarifier (DS + S), minimum tillage (MT) and conventional tillage (CT). DS presented higher values than CT in culturable total fungi (26.33 × 105 vs. 2.33 × 105 CFU g−1 dry soil), total bacteria (182 × 107 vs. 64 × 107 CFU g−1 dry soil), microbial respiration (0.77 mg CO2 g−1 week−1 vs. 0.45 mg CO2 g−1 week−1) and fluorescein diacetate (FDA) hydrolysis (4.17 ug fluorescein g−1 h−1 vs. 1.70 ug fluorescein g−1 h−1 in CT. Fungal and bacterial community fingerprints, by terminal restriction fragment length polymorphism (T-RFLP) analysis, of Intergenic spacer regions of rRNA and 16S rRNA genes, respectively, were influenced by the tillage system. Also FAME (fatty acid methyl ester) profiles showed that microbial community structure in DS and CT was clearly different. DS samples contained significantly higher total microbial biomass than the other tillage treatments, but there were no significant differences in fungal biomass or any consistent trend with respect to stress index. Our results showed that microbial communities were more abundant and active in DS than in CT in response to high nutrient content in soil. Indeed, DS systems presented higher soil OM, total N, K and Ca than CT. Electrical conductivity and aggregate stability (AS) were also improved by DS. Soybean grown in high-quality soil was not affected by charcoal rot, however, under CT, disease incidence in soybean was 54%. These differences were correlated to the higher microbial abundance and activity under DS, the biological component being a key factor determining soil capacity to suppress the soilborne pathogen. |
| format | info:ar-repo/semantics/artículo |
| id | INTA4302 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA43022019-01-21T14:50:55Z Long-term effect of tillage systems on soil microbiological, chemical and physical parameters and the incidence of charcoal rot by Macrophomina phaseolina (Tassi) Goid in soybean Perez Brandan, Carolina Arzeno, Jose Luis Huidobro, Dina Jorgelina Grumberg, Betiana Clarisa Conforto, Erica Cinthia Hilton, Sally Bending, Gary D. Meriles, Jose Manuel Vargas Gil, Silvina Labranza Propiedades Físico - Químicas Suelo Soja Macrophomina phaseolina Biología del Suelo Hongos del Suelo Tillage Soil Chemicophysical Properties Soybeans Soil Biology Soil Fungi Sistemas de Labranza A 20-year field experiment was employed with the aim of evaluating the effect of tillage systems on biological, chemical and physical aspects of the soil, and to establish whether there was a correlation of these parameters with the incidence of charcoal rot (Macrophomina phaseolina) of soybean and crop yield. The tillage systems evaluated were direct seeding (DS), DS + scarifier (DS + S), minimum tillage (MT) and conventional tillage (CT). DS presented higher values than CT in culturable total fungi (26.33 × 105 vs. 2.33 × 105 CFU g−1 dry soil), total bacteria (182 × 107 vs. 64 × 107 CFU g−1 dry soil), microbial respiration (0.77 mg CO2 g−1 week−1 vs. 0.45 mg CO2 g−1 week−1) and fluorescein diacetate (FDA) hydrolysis (4.17 ug fluorescein g−1 h−1 vs. 1.70 ug fluorescein g−1 h−1 in CT. Fungal and bacterial community fingerprints, by terminal restriction fragment length polymorphism (T-RFLP) analysis, of Intergenic spacer regions of rRNA and 16S rRNA genes, respectively, were influenced by the tillage system. Also FAME (fatty acid methyl ester) profiles showed that microbial community structure in DS and CT was clearly different. DS samples contained significantly higher total microbial biomass than the other tillage treatments, but there were no significant differences in fungal biomass or any consistent trend with respect to stress index. Our results showed that microbial communities were more abundant and active in DS than in CT in response to high nutrient content in soil. Indeed, DS systems presented higher soil OM, total N, K and Ca than CT. Electrical conductivity and aggregate stability (AS) were also improved by DS. Soybean grown in high-quality soil was not affected by charcoal rot, however, under CT, disease incidence in soybean was 54%. These differences were correlated to the higher microbial abundance and activity under DS, the biological component being a key factor determining soil capacity to suppress the soilborne pathogen. EEA Salta Fil: Perez Brandan, Carolina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina Fil: Arzeno, Jose Luis. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina Fil: Huidobro, Dina Jorgelina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina Fil: Grumberg, Betiana Clarisa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina Fil: Conforto, Erica Cinthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Hilton, Sally. University of Warwick. School of Life Sciences; Reino Unido Fil: Bending, Gary D. University of Warwick. School of Life Sciences; Reino Unido Fil: Meriles, Jose Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal; Argentina Fil: Vargas Gil, Silvina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina 2019-01-21T14:48:11Z 2019-01-21T14:48:11Z 2012-10 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.sciencedirect.com/science/article/pii/S0261219412001159 http://hdl.handle.net/20.500.12123/4302 0261-2194 https://doi.org/10.1016/j.cropro.2012.04.018 eng info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Crop Protection 40 : 73-82 /October 2012) |
| spellingShingle | Labranza Propiedades Físico - Químicas Suelo Soja Macrophomina phaseolina Biología del Suelo Hongos del Suelo Tillage Soil Chemicophysical Properties Soybeans Soil Biology Soil Fungi Sistemas de Labranza Perez Brandan, Carolina Arzeno, Jose Luis Huidobro, Dina Jorgelina Grumberg, Betiana Clarisa Conforto, Erica Cinthia Hilton, Sally Bending, Gary D. Meriles, Jose Manuel Vargas Gil, Silvina Long-term effect of tillage systems on soil microbiological, chemical and physical parameters and the incidence of charcoal rot by Macrophomina phaseolina (Tassi) Goid in soybean |
| title | Long-term effect of tillage systems on soil microbiological, chemical and physical parameters and the incidence of charcoal rot by Macrophomina phaseolina (Tassi) Goid in soybean |
| title_full | Long-term effect of tillage systems on soil microbiological, chemical and physical parameters and the incidence of charcoal rot by Macrophomina phaseolina (Tassi) Goid in soybean |
| title_fullStr | Long-term effect of tillage systems on soil microbiological, chemical and physical parameters and the incidence of charcoal rot by Macrophomina phaseolina (Tassi) Goid in soybean |
| title_full_unstemmed | Long-term effect of tillage systems on soil microbiological, chemical and physical parameters and the incidence of charcoal rot by Macrophomina phaseolina (Tassi) Goid in soybean |
| title_short | Long-term effect of tillage systems on soil microbiological, chemical and physical parameters and the incidence of charcoal rot by Macrophomina phaseolina (Tassi) Goid in soybean |
| title_sort | long term effect of tillage systems on soil microbiological chemical and physical parameters and the incidence of charcoal rot by macrophomina phaseolina tassi goid in soybean |
| topic | Labranza Propiedades Físico - Químicas Suelo Soja Macrophomina phaseolina Biología del Suelo Hongos del Suelo Tillage Soil Chemicophysical Properties Soybeans Soil Biology Soil Fungi Sistemas de Labranza |
| url | https://www.sciencedirect.com/science/article/pii/S0261219412001159 http://hdl.handle.net/20.500.12123/4302 https://doi.org/10.1016/j.cropro.2012.04.018 |
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