Detection transposable elements in Botrytis cinerea in latent infection stage from symptomless apples
Objective: To detect Botrytis cinerea (B. cinerea) latent infections on apples before storage, which is essential for effective control strategies in the fruit postharvest industry. Methods: In the present study, a polymerase chain reaction detection method, based on primers designed on B. cinerea t...
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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Hainan Medical University
2019
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| Subjects: | |
| Online Access: | http://oaji.net/articles/2015/2154-1445415270.pdf http://hdl.handle.net/20.500.12123/4297 https://doi.org/10.12980/JCLM.2.2014JCLM-2013-0005 |
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| author | Fernandez, Jorge Gastón Fernández Baldo, Martín Alejandro Muñoz, Claudio Javier Salinas, Eloy Raba, Julio Sanz Ferramola, Maria Isabel |
| author_browse | Fernandez, Jorge Gastón Fernández Baldo, Martín Alejandro Muñoz, Claudio Javier Raba, Julio Salinas, Eloy Sanz Ferramola, Maria Isabel |
| author_facet | Fernandez, Jorge Gastón Fernández Baldo, Martín Alejandro Muñoz, Claudio Javier Salinas, Eloy Raba, Julio Sanz Ferramola, Maria Isabel |
| author_sort | Fernandez, Jorge Gastón |
| collection | INTA Digital |
| description | Objective: To detect Botrytis cinerea (B. cinerea) latent infections on apples before storage, which is essential for effective control strategies in the fruit postharvest industry. Methods: In the present study, a polymerase chain reaction detection method, based on primers designed on B. cinerea transposable elements (boty and flipper) and intergenic spacer region as internal control, were utilized to reveal the presence of symptomless infections on apple fruits. This molecular method proved to be highly specific and sensitive in detecting latent infections. It revealed the presence of the pathogen in 83% of the samples from infected apples with 104 conidia/ mL, whereas those infected with 106 conidia/mL detected 94% as compared to the traditional method that revealed the pathogen in 40% and 66% of the samples inoculated with 104 and 106 conidia/mL respectively. Furthermore, the method characterized B. cinerea as subpopulation transposa-type by the presence of the transposable elements boty and flipper Results: The results obtained from DNA quantification method were compared with enzymelinked immunosorbent assay and these studies showed good correlation. Therefore our method has important advantages compared with others detection methods for B. cinerea, because the proposed methodology allowed distinguishes between its two subpopulations (vacuma and transposa) and this would allow establish possible appropriate control strategies. Conclusions: Finally, the method can be an interesting alternative for its possible application in the phytosanitary programs of the fruit industry worldwide. |
| format | info:ar-repo/semantics/artículo |
| id | INTA4297 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Hainan Medical University |
| publisherStr | Hainan Medical University |
| record_format | dspace |
| spelling | INTA42972019-01-21T11:47:02Z Detection transposable elements in Botrytis cinerea in latent infection stage from symptomless apples Fernandez, Jorge Gastón Fernández Baldo, Martín Alejandro Muñoz, Claudio Javier Salinas, Eloy Raba, Julio Sanz Ferramola, Maria Isabel Botrytis Cinerea Manzana Hongos Enfermedades de las Plantas Enfermedades Postcosecha Infecciones Latentes Apples Fungi Plant Diseases Postharvest Decay Latent Infections Enfermedades durante Almacenamiento Objective: To detect Botrytis cinerea (B. cinerea) latent infections on apples before storage, which is essential for effective control strategies in the fruit postharvest industry. Methods: In the present study, a polymerase chain reaction detection method, based on primers designed on B. cinerea transposable elements (boty and flipper) and intergenic spacer region as internal control, were utilized to reveal the presence of symptomless infections on apple fruits. This molecular method proved to be highly specific and sensitive in detecting latent infections. It revealed the presence of the pathogen in 83% of the samples from infected apples with 104 conidia/ mL, whereas those infected with 106 conidia/mL detected 94% as compared to the traditional method that revealed the pathogen in 40% and 66% of the samples inoculated with 104 and 106 conidia/mL respectively. Furthermore, the method characterized B. cinerea as subpopulation transposa-type by the presence of the transposable elements boty and flipper Results: The results obtained from DNA quantification method were compared with enzymelinked immunosorbent assay and these studies showed good correlation. Therefore our method has important advantages compared with others detection methods for B. cinerea, because the proposed methodology allowed distinguishes between its two subpopulations (vacuma and transposa) and this would allow establish possible appropriate control strategies. Conclusions: Finally, the method can be an interesting alternative for its possible application in the phytosanitary programs of the fruit industry worldwide. EEA Mendoza Fil: Fernández, Jorge Gastón. Universidad Nacional de San Luis. Facultad de Quimica, Bioquimica y Farmacia. Departamento de Quimica. Area de Quimica Analitica; Argentina Fil: Fernández Baldo, Martín Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina. Universidad Nacional de San Luis. Departamento de Química. Instituto de Química de San Luis; Argentina Fil: Muñoz, Claudio Javier. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina Fil: Salinas, Eloy. Universidad Nacional de San Luis. Facultad de Quimica, Bioquimica y Farmacia. Departamento de Quimica. Area de Quimica Analitica; Argentina Fil: Raba, Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina. Universidad Nacional de San Luis. Departamento de Química. Instituto de Química de San Luis; Argentina Fil: Sanz Ferramola, Maria Isabel. Universidad Nacional de San Luis. Facultad de Quimica, Bioquimica y Farmacia. Departamento de Quimica. Area de Quimica Analitica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina. Universidad Nacional de San Luis. Departamento de Química. Instituto de Química de San Luis; Argentina 2019-01-21T11:45:10Z 2019-01-21T11:45:10Z 2014 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://oaji.net/articles/2015/2154-1445415270.pdf http://hdl.handle.net/20.500.12123/4297 2309-5288 2309-6152 https://doi.org/10.12980/JCLM.2.2014JCLM-2013-0005 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Hainan Medical University Journal of Coastal Life Medicine 2 (2) : 125-131 (2014) |
| spellingShingle | Botrytis Cinerea Manzana Hongos Enfermedades de las Plantas Enfermedades Postcosecha Infecciones Latentes Apples Fungi Plant Diseases Postharvest Decay Latent Infections Enfermedades durante Almacenamiento Fernandez, Jorge Gastón Fernández Baldo, Martín Alejandro Muñoz, Claudio Javier Salinas, Eloy Raba, Julio Sanz Ferramola, Maria Isabel Detection transposable elements in Botrytis cinerea in latent infection stage from symptomless apples |
| title | Detection transposable elements in Botrytis cinerea in latent infection stage from symptomless apples |
| title_full | Detection transposable elements in Botrytis cinerea in latent infection stage from symptomless apples |
| title_fullStr | Detection transposable elements in Botrytis cinerea in latent infection stage from symptomless apples |
| title_full_unstemmed | Detection transposable elements in Botrytis cinerea in latent infection stage from symptomless apples |
| title_short | Detection transposable elements in Botrytis cinerea in latent infection stage from symptomless apples |
| title_sort | detection transposable elements in botrytis cinerea in latent infection stage from symptomless apples |
| topic | Botrytis Cinerea Manzana Hongos Enfermedades de las Plantas Enfermedades Postcosecha Infecciones Latentes Apples Fungi Plant Diseases Postharvest Decay Latent Infections Enfermedades durante Almacenamiento |
| url | http://oaji.net/articles/2015/2154-1445415270.pdf http://hdl.handle.net/20.500.12123/4297 https://doi.org/10.12980/JCLM.2.2014JCLM-2013-0005 |
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