Exploring the Intrinsic Limits of Nitrogenase Transfer from Bacteria to Eukaryotes
Biological nitrogen fixation is widespread among the Eubacteria and Archae domains but completely absent in eukaryotes. The lack of lateral transfer of nitrogen-fixation genes from prokaryotes to eukaryotes has been partially attributed to the physiological requirements necessary for the function of...
| Main Authors: | , , |
|---|---|
| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
| Published: |
Springer
2019
|
| Subjects: | |
| Online Access: | https://link.springer.com/article/10.1007/s00239-013-9578-8 http://hdl.handle.net/20.500.12123/4296 https://doi.org/10.1007/s00239-013-9578-8 |
| _version_ | 1855035296107724800 |
|---|---|
| author | Soto, Gabriela Cynthia Fox, Ana Romina Ayub, Nicolás Daniel |
| author_browse | Ayub, Nicolás Daniel Fox, Ana Romina Soto, Gabriela Cynthia |
| author_facet | Soto, Gabriela Cynthia Fox, Ana Romina Ayub, Nicolás Daniel |
| author_sort | Soto, Gabriela Cynthia |
| collection | INTA Digital |
| description | Biological nitrogen fixation is widespread among the Eubacteria and Archae domains but completely absent in eukaryotes. The lack of lateral transfer of nitrogen-fixation genes from prokaryotes to eukaryotes has been partially attributed to the physiological requirements necessary for the function of the nitrogenase complex. However, symbiotic bacterial nitrogenase activity is protected by the nodule, a plant structure whose organogenesis can be trigged in the absence of bacteria. To explore the intrinsic potentiality of this plant organ, we generated rhizobium-independent nodules in alfalfa by overexpressing the MsDMI3 kinase lacking the autoinhibitory domain. These transgenic nodules showed similar levels of leghemoglobin, free oxygen, ATP, and NADPH to those of efficient Sinorhizobium meliloti B399-infected nodules, suggesting that the rhizobium-independent nodules can provide an optimal microenvironment for nitrogenase activity. Finally, we discuss the intrinsic evolutionary constraints on transfer of nitrogen-fixation genes between bacteria and eukaryotes. |
| format | info:ar-repo/semantics/artículo |
| id | INTA4296 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA42962019-01-18T14:56:28Z Exploring the Intrinsic Limits of Nitrogenase Transfer from Bacteria to Eukaryotes Soto, Gabriela Cynthia Fox, Ana Romina Ayub, Nicolás Daniel Fijación Biológica del Nitrógeno Eukaryota Oxígeno Biological Nitrogen Fixation Oxygen Eukaryotes Biological nitrogen fixation is widespread among the Eubacteria and Archae domains but completely absent in eukaryotes. The lack of lateral transfer of nitrogen-fixation genes from prokaryotes to eukaryotes has been partially attributed to the physiological requirements necessary for the function of the nitrogenase complex. However, symbiotic bacterial nitrogenase activity is protected by the nodule, a plant structure whose organogenesis can be trigged in the absence of bacteria. To explore the intrinsic potentiality of this plant organ, we generated rhizobium-independent nodules in alfalfa by overexpressing the MsDMI3 kinase lacking the autoinhibitory domain. These transgenic nodules showed similar levels of leghemoglobin, free oxygen, ATP, and NADPH to those of efficient Sinorhizobium meliloti B399-infected nodules, suggesting that the rhizobium-independent nodules can provide an optimal microenvironment for nitrogenase activity. Finally, we discuss the intrinsic evolutionary constraints on transfer of nitrogen-fixation genes between bacteria and eukaryotes. Instituto de Genética Fil: Soto, Gabriela Cynthia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina Fil: Fox, Ana Romina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Ayub, Nicolás Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2019-01-18T14:53:36Z 2019-01-18T14:53:36Z 2013-08 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://link.springer.com/article/10.1007/s00239-013-9578-8 http://hdl.handle.net/20.500.12123/4296 0022-2844 1432-1432 https://doi.org/10.1007/s00239-013-9578-8 eng info:eu-repo/semantics/restrictedAccess application/pdf Springer Journal of Molecular Evolution 77 (1–2) : 3–7 (August 2013) |
| spellingShingle | Fijación Biológica del Nitrógeno Eukaryota Oxígeno Biological Nitrogen Fixation Oxygen Eukaryotes Soto, Gabriela Cynthia Fox, Ana Romina Ayub, Nicolás Daniel Exploring the Intrinsic Limits of Nitrogenase Transfer from Bacteria to Eukaryotes |
| title | Exploring the Intrinsic Limits of Nitrogenase Transfer from Bacteria to Eukaryotes |
| title_full | Exploring the Intrinsic Limits of Nitrogenase Transfer from Bacteria to Eukaryotes |
| title_fullStr | Exploring the Intrinsic Limits of Nitrogenase Transfer from Bacteria to Eukaryotes |
| title_full_unstemmed | Exploring the Intrinsic Limits of Nitrogenase Transfer from Bacteria to Eukaryotes |
| title_short | Exploring the Intrinsic Limits of Nitrogenase Transfer from Bacteria to Eukaryotes |
| title_sort | exploring the intrinsic limits of nitrogenase transfer from bacteria to eukaryotes |
| topic | Fijación Biológica del Nitrógeno Eukaryota Oxígeno Biological Nitrogen Fixation Oxygen Eukaryotes |
| url | https://link.springer.com/article/10.1007/s00239-013-9578-8 http://hdl.handle.net/20.500.12123/4296 https://doi.org/10.1007/s00239-013-9578-8 |
| work_keys_str_mv | AT sotogabrielacynthia exploringtheintrinsiclimitsofnitrogenasetransferfrombacteriatoeukaryotes AT foxanaromina exploringtheintrinsiclimitsofnitrogenasetransferfrombacteriatoeukaryotes AT ayubnicolasdaniel exploringtheintrinsiclimitsofnitrogenasetransferfrombacteriatoeukaryotes |