Association between spoligotype-VNTR types and virulence of Mycobacterium bovis in cattle
Mycobacterium bovis is the causative agent of bovine tuberculosis, a disease that affects approximately 5% of Argentine cattle. The aim of this research was to study if it is possible to infer the degree of virulence of different M. bovis genotypes based on scorified observations of tuberculosis les...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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Taylor & Francis
2019
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| Online Access: | https://www.tandfonline.com/doi/full/10.4161/viru.27193 http://hdl.handle.net/20.500.12123/4294 https://doi.org/10.4161/viru.27193 |
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| author | Garbaccio, Sergio Gabriel Macias, Analía Shimizu, Ernesto Paolicchi, Fernando Pezzone, Natalia Zapata, Laura Abdala, Alejandro Ariel Tarabla, Hector Dante Peyru, Maite Caimi, Karina Cynthia Zumarraga, Martin Jose Canal, Ana María Cataldi, Angel Adrian Magnano, Gabriel |
| author_browse | Abdala, Alejandro Ariel Caimi, Karina Cynthia Canal, Ana María Cataldi, Angel Adrian Garbaccio, Sergio Gabriel Macias, Analía Magnano, Gabriel Paolicchi, Fernando Peyru, Maite Pezzone, Natalia Shimizu, Ernesto Tarabla, Hector Dante Zapata, Laura Zumarraga, Martin Jose |
| author_facet | Garbaccio, Sergio Gabriel Macias, Analía Shimizu, Ernesto Paolicchi, Fernando Pezzone, Natalia Zapata, Laura Abdala, Alejandro Ariel Tarabla, Hector Dante Peyru, Maite Caimi, Karina Cynthia Zumarraga, Martin Jose Canal, Ana María Cataldi, Angel Adrian Magnano, Gabriel |
| author_sort | Garbaccio, Sergio Gabriel |
| collection | INTA Digital |
| description | Mycobacterium bovis is the causative agent of bovine tuberculosis, a disease that affects approximately 5% of Argentine cattle. The aim of this research was to study if it is possible to infer the degree of virulence of different M. bovis genotypes based on scorified observations of tuberculosis lesions in cattle. In this study, we performed association analyses between several parameters with tuberculosis lesions: M. bovis genotype, degree of progression of tuberculosis, and animal age. For this purpose, the genotype was determined by spoligotyping and the degree of bovine tuberculosis gross lesion was quantified with a score based on clinical observations (number, size, and location of granulomas along with histopathologic features). This study was performed with naturally infected cattle of slaughterhouses from three provinces in Argentina. A total of 265 M. bovis isolates were obtained from 378 pathological lesion samples and 192 spoligotyping and VNTR (based on ETR sequences) typing patterns were obtained. SB0140 was the most predominant spoligotype, followed by SB0145. The spoligotype with the highest lesion score was SB0273 (median score of 27 ± 4.46), followed by SB0520 (18 ± 5.8). Furthermore, the most common spoligotype, SB0140, had a median score of 11 ± 0.74. Finally, the spoligotype with the lowest score was SB0145 (8 ± 1.0). ETR typing of SB0140, SB0145, SB0273, and SB0520 did not subdivide the lesion scores in those spoligotypes. In conclusion, SB0273 and SB0520 were the spoligotypes with the strongest association with hypervirulence and both spoligotypes were only found in Río Cuarto at the south of Córdoba province. Interestingly, there is no other report of any of these spoligotyes in Latin America. |
| format | info:ar-repo/semantics/artículo |
| id | INTA4294 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Taylor & Francis |
| publisherStr | Taylor & Francis |
| record_format | dspace |
| spelling | INTA42942019-01-18T13:49:48Z Association between spoligotype-VNTR types and virulence of Mycobacterium bovis in cattle Garbaccio, Sergio Gabriel Macias, Analía Shimizu, Ernesto Paolicchi, Fernando Pezzone, Natalia Zapata, Laura Abdala, Alejandro Ariel Tarabla, Hector Dante Peyru, Maite Caimi, Karina Cynthia Zumarraga, Martin Jose Canal, Ana María Cataldi, Angel Adrian Magnano, Gabriel Mycobacterium Bovis Ganado Bovino Enfermedades de los Animales Virulencia Genotipos Cattle Animal Diseases Virulence Genotypes Mycobacterium bovis is the causative agent of bovine tuberculosis, a disease that affects approximately 5% of Argentine cattle. The aim of this research was to study if it is possible to infer the degree of virulence of different M. bovis genotypes based on scorified observations of tuberculosis lesions in cattle. In this study, we performed association analyses between several parameters with tuberculosis lesions: M. bovis genotype, degree of progression of tuberculosis, and animal age. For this purpose, the genotype was determined by spoligotyping and the degree of bovine tuberculosis gross lesion was quantified with a score based on clinical observations (number, size, and location of granulomas along with histopathologic features). This study was performed with naturally infected cattle of slaughterhouses from three provinces in Argentina. A total of 265 M. bovis isolates were obtained from 378 pathological lesion samples and 192 spoligotyping and VNTR (based on ETR sequences) typing patterns were obtained. SB0140 was the most predominant spoligotype, followed by SB0145. The spoligotype with the highest lesion score was SB0273 (median score of 27 ± 4.46), followed by SB0520 (18 ± 5.8). Furthermore, the most common spoligotype, SB0140, had a median score of 11 ± 0.74. Finally, the spoligotype with the lowest score was SB0145 (8 ± 1.0). ETR typing of SB0140, SB0145, SB0273, and SB0520 did not subdivide the lesion scores in those spoligotypes. In conclusion, SB0273 and SB0520 were the spoligotypes with the strongest association with hypervirulence and both spoligotypes were only found in Río Cuarto at the south of Córdoba province. Interestingly, there is no other report of any of these spoligotyes in Latin America. Instituto de Patobiología Fil: Garbaccio, Sergio Gabriel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina Fil: Macias, Analía. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina Fil: Shimizu, Ernesto. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Laboratorio de Bacteriología; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina Fil: Paolicchi, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Laboratorio de Bacteriología; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina Fil: Pezzone, Natalia. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Patología Básica; Argentina Fil: Magnano, Gabriel. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina Fil: Zapata, Laura. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina Fil: Abdala, Alejandro Ariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina Fil: Tarabla, Hector Dante. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina Fil: Peyru, Maite. Entre Ríos (provincia). Secretaría de la Producción de Entre Ríos. Dirección General de Producción Animal; Argentina Fil: Caimi, Karina Cynthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Canal, Ana María. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Patología Básica; Argentina Fil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina 2019-01-18T13:48:36Z 2019-01-18T13:48:36Z 2014-01 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.tandfonline.com/doi/full/10.4161/viru.27193 http://hdl.handle.net/20.500.12123/4294 2150-5594 2150-5608 https://doi.org/10.4161/viru.27193 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Taylor & Francis Virulence 5 (2) : 297-302 (2014) |
| spellingShingle | Mycobacterium Bovis Ganado Bovino Enfermedades de los Animales Virulencia Genotipos Cattle Animal Diseases Virulence Genotypes Garbaccio, Sergio Gabriel Macias, Analía Shimizu, Ernesto Paolicchi, Fernando Pezzone, Natalia Zapata, Laura Abdala, Alejandro Ariel Tarabla, Hector Dante Peyru, Maite Caimi, Karina Cynthia Zumarraga, Martin Jose Canal, Ana María Cataldi, Angel Adrian Magnano, Gabriel Association between spoligotype-VNTR types and virulence of Mycobacterium bovis in cattle |
| title | Association between spoligotype-VNTR types and virulence of Mycobacterium bovis in cattle |
| title_full | Association between spoligotype-VNTR types and virulence of Mycobacterium bovis in cattle |
| title_fullStr | Association between spoligotype-VNTR types and virulence of Mycobacterium bovis in cattle |
| title_full_unstemmed | Association between spoligotype-VNTR types and virulence of Mycobacterium bovis in cattle |
| title_short | Association between spoligotype-VNTR types and virulence of Mycobacterium bovis in cattle |
| title_sort | association between spoligotype vntr types and virulence of mycobacterium bovis in cattle |
| topic | Mycobacterium Bovis Ganado Bovino Enfermedades de los Animales Virulencia Genotipos Cattle Animal Diseases Virulence Genotypes |
| url | https://www.tandfonline.com/doi/full/10.4161/viru.27193 http://hdl.handle.net/20.500.12123/4294 https://doi.org/10.4161/viru.27193 |
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