Immunogenicity of hypothetical highly conserved proteins as novel antigens in Anaplasma marginale
Anaplasma marginale is a tick-transmitted Gram-negative intraerythrocytic bacterium and the etiological agent of bovine Anaplasmosis. Even though considerable research efforts have been undertaken, Anaplasmosis vaccine development remains a challenging field. Outer-membrane-specific antigens respons...
| Autores principales: | , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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Springer
2019
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| Acceso en línea: | https://link.springer.com/article/10.1007/s00284-013-0475-6 http://hdl.handle.net/20.500.12123/4291 https://doi.org/10.1007/s00284-013-0475-6 |
| _version_ | 1855483403831345152 |
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| author | Nuñez, Pablo Alfredo Moretta, Rosalia Ester Ruybal, Paula Wilkowsky, Silvina Elizabeth Farber, Marisa Diana |
| author_browse | Farber, Marisa Diana Moretta, Rosalia Ester Nuñez, Pablo Alfredo Ruybal, Paula Wilkowsky, Silvina Elizabeth |
| author_facet | Nuñez, Pablo Alfredo Moretta, Rosalia Ester Ruybal, Paula Wilkowsky, Silvina Elizabeth Farber, Marisa Diana |
| author_sort | Nuñez, Pablo Alfredo |
| collection | INTA Digital |
| description | Anaplasma marginale is a tick-transmitted Gram-negative intraerythrocytic bacterium and the etiological agent of bovine Anaplasmosis. Even though considerable research efforts have been undertaken, Anaplasmosis vaccine development remains a challenging field. Outer-membrane-specific antigens responsible for the ability of more complex immunogens could have a significant role in the protective response. Thus, the identification of outer-membrane antigens represents a major goal in the development of bacterial vaccines. Considering that 40 % of the annotated proteins in A. marginale remain as hypothetical, we selected three candidate antigens, AM1108, AM127, and AM216 based on experimental evidence, in silico structure prediction of β-barrel outer membrane, and orthology clustering. Sequence alignment and analysis demonstrated a high degree of conservation for the three proteins between the isolates from Argentina compared to the American strains. We confirmed the transcription of the three genes in the intraerythrocytic stage. AM1108 and AM216 recombinant proteins elicited specific T-cell response proliferation and a significant rise in TNF-α and IFN-γ transcript levels, respectively. Only AM1108 was able to be recognized by specific antibodies from infected bovines. This study allowed the identification of new candidate components of the outer-membrane fraction of A. marginale. Further studies will be required to analyze their potential as effective antigens for being included in rational vaccine strategies. |
| format | Artículo |
| id | INTA4291 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA42912019-01-18T12:05:34Z Immunogenicity of hypothetical highly conserved proteins as novel antigens in Anaplasma marginale Nuñez, Pablo Alfredo Moretta, Rosalia Ester Ruybal, Paula Wilkowsky, Silvina Elizabeth Farber, Marisa Diana Anaplasma Marginale Inmunogenética Antígenos Antigenos Bacterianos Vacuna Immunogenetics Antigens Bacterial Antigens Vaccines Anaplasma marginale is a tick-transmitted Gram-negative intraerythrocytic bacterium and the etiological agent of bovine Anaplasmosis. Even though considerable research efforts have been undertaken, Anaplasmosis vaccine development remains a challenging field. Outer-membrane-specific antigens responsible for the ability of more complex immunogens could have a significant role in the protective response. Thus, the identification of outer-membrane antigens represents a major goal in the development of bacterial vaccines. Considering that 40 % of the annotated proteins in A. marginale remain as hypothetical, we selected three candidate antigens, AM1108, AM127, and AM216 based on experimental evidence, in silico structure prediction of β-barrel outer membrane, and orthology clustering. Sequence alignment and analysis demonstrated a high degree of conservation for the three proteins between the isolates from Argentina compared to the American strains. We confirmed the transcription of the three genes in the intraerythrocytic stage. AM1108 and AM216 recombinant proteins elicited specific T-cell response proliferation and a significant rise in TNF-α and IFN-γ transcript levels, respectively. Only AM1108 was able to be recognized by specific antibodies from infected bovines. This study allowed the identification of new candidate components of the outer-membrane fraction of A. marginale. Further studies will be required to analyze their potential as effective antigens for being included in rational vaccine strategies. Instituto de Biotecnología Fil: Nuñez, Pablo Alfredo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Moretta, Rosalia Ester. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Ruybal, Paula. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Wilkowsky, Silvina Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Farber, Marisa Diana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina 2019-01-18T12:04:03Z 2019-01-18T12:04:03Z 2014-03 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://link.springer.com/article/10.1007/s00284-013-0475-6 http://hdl.handle.net/20.500.12123/4291 0343-8651 1432-0991 https://doi.org/10.1007/s00284-013-0475-6 eng info:eu-repo/semantics/restrictedAccess application/pdf Springer Current Microbiology 68 (3) : 269–277 (March 2014) |
| spellingShingle | Anaplasma Marginale Inmunogenética Antígenos Antigenos Bacterianos Vacuna Immunogenetics Antigens Bacterial Antigens Vaccines Nuñez, Pablo Alfredo Moretta, Rosalia Ester Ruybal, Paula Wilkowsky, Silvina Elizabeth Farber, Marisa Diana Immunogenicity of hypothetical highly conserved proteins as novel antigens in Anaplasma marginale |
| title | Immunogenicity of hypothetical highly conserved proteins as novel antigens in Anaplasma marginale |
| title_full | Immunogenicity of hypothetical highly conserved proteins as novel antigens in Anaplasma marginale |
| title_fullStr | Immunogenicity of hypothetical highly conserved proteins as novel antigens in Anaplasma marginale |
| title_full_unstemmed | Immunogenicity of hypothetical highly conserved proteins as novel antigens in Anaplasma marginale |
| title_short | Immunogenicity of hypothetical highly conserved proteins as novel antigens in Anaplasma marginale |
| title_sort | immunogenicity of hypothetical highly conserved proteins as novel antigens in anaplasma marginale |
| topic | Anaplasma Marginale Inmunogenética Antígenos Antigenos Bacterianos Vacuna Immunogenetics Antigens Bacterial Antigens Vaccines |
| url | https://link.springer.com/article/10.1007/s00284-013-0475-6 http://hdl.handle.net/20.500.12123/4291 https://doi.org/10.1007/s00284-013-0475-6 |
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