Isolation of Pseudobutyrivibrio ruminis and Pseudobutyrivibrio xylanivorans from rumen of Creole goats fed native forage diet

We isolated and identified functional groups of bacteria in the rumen of Creole goats involved in ruminal fermentation of native forage shrubs. The functional bacterial groups were evaluated by comparing the total viable, total anaerobic, cellulolytic, hemicellulolytic, and amylolytic bacterial coun...

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Main Authors: Grilli, Diego Javier, Ceron Cucchi, Maria Esperanza, Paez Lama, Sebastián Antonio, Egea, Angela Vanina, Schnittger, Leonhard, Cravero, Silvio Lorenzo Pedro, Sosa Escudero, Miguel Angel, Allegretti, Liliana Inés, Arenas, Graciela Nora
Format: info:ar-repo/semantics/artículo
Language:Inglés
Published: Springer 2019
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Online Access:https://link.springer.com/article/10.1007/s12223-012-0219-1
http://hdl.handle.net/20.500.12123/4275
https://doi.org/10.1007/s12223-012-0219-1
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author Grilli, Diego Javier
Ceron Cucchi, Maria Esperanza
Paez Lama, Sebastián Antonio
Egea, Angela Vanina
Schnittger, Leonhard
Cravero, Silvio Lorenzo Pedro
Sosa Escudero, Miguel Angel
Allegretti, Liliana Inés
Arenas, Graciela Nora
author_browse Allegretti, Liliana Inés
Arenas, Graciela Nora
Ceron Cucchi, Maria Esperanza
Cravero, Silvio Lorenzo Pedro
Egea, Angela Vanina
Grilli, Diego Javier
Paez Lama, Sebastián Antonio
Schnittger, Leonhard
Sosa Escudero, Miguel Angel
author_facet Grilli, Diego Javier
Ceron Cucchi, Maria Esperanza
Paez Lama, Sebastián Antonio
Egea, Angela Vanina
Schnittger, Leonhard
Cravero, Silvio Lorenzo Pedro
Sosa Escudero, Miguel Angel
Allegretti, Liliana Inés
Arenas, Graciela Nora
author_sort Grilli, Diego Javier
collection INTA Digital
description We isolated and identified functional groups of bacteria in the rumen of Creole goats involved in ruminal fermentation of native forage shrubs. The functional bacterial groups were evaluated by comparing the total viable, total anaerobic, cellulolytic, hemicellulolytic, and amylolytic bacterial counts in the samples taken from fistulated goats fed native forage diet (Atriplex lampa and Prosopis flexuosa). Alfalfa hay and corn were used as control diet. The roll tubes method increased the possibility of isolating and 16S rDNA gene sequencing allowed definitive identification of bacterial species involved in the ruminal fermentation. The starch and fiber contents of the diets influenced the number of total anaerobic bacteria and fibrolytic and amylolytic functional groups. Pseudobutyrivibrio ruminis and Pseudobutyrivibrio xylanivorans were the main species isolated and identified. The identification of bacterial strains involved in the rumen fermentation helps to explain the ability of these animals to digest fiber plant cell wall contained in native forage species.
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institution Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina)
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publishDate 2019
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spelling INTA42752019-01-16T14:58:08Z Isolation of Pseudobutyrivibrio ruminis and Pseudobutyrivibrio xylanivorans from rumen of Creole goats fed native forage diet Grilli, Diego Javier Ceron Cucchi, Maria Esperanza Paez Lama, Sebastián Antonio Egea, Angela Vanina Schnittger, Leonhard Cravero, Silvio Lorenzo Pedro Sosa Escudero, Miguel Angel Allegretti, Liliana Inés Arenas, Graciela Nora Caprinos Razas (animales) Digestión Ruminal Forrajes Alimentación de los Animales Goats Breeds (animals) Rumen Digestion Forage Animal Feeding Raza Criolla Pseudobutyrivibrio ruminis Pseudobutyrivibrio xylanivorans We isolated and identified functional groups of bacteria in the rumen of Creole goats involved in ruminal fermentation of native forage shrubs. The functional bacterial groups were evaluated by comparing the total viable, total anaerobic, cellulolytic, hemicellulolytic, and amylolytic bacterial counts in the samples taken from fistulated goats fed native forage diet (Atriplex lampa and Prosopis flexuosa). Alfalfa hay and corn were used as control diet. The roll tubes method increased the possibility of isolating and 16S rDNA gene sequencing allowed definitive identification of bacterial species involved in the ruminal fermentation. The starch and fiber contents of the diets influenced the number of total anaerobic bacteria and fibrolytic and amylolytic functional groups. Pseudobutyrivibrio ruminis and Pseudobutyrivibrio xylanivorans were the main species isolated and identified. The identification of bacterial strains involved in the rumen fermentation helps to explain the ability of these animals to digest fiber plant cell wall contained in native forage species. Instituto de Patobiología Fil: Grilli, Diego Javier. Universidad Juan Agustín Maza. Facultad de Ciencias Veterinarias y Ambientales. Cátedra de Bacteriología; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Cátedra de Microbiología; Argentina. Fil: Ceron Cucchi, Maria Esperanza. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina Fil: Paez Lama, Sebastián Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Argentino de Investigaciones de las Zonas Áridas. Provincia de Mendoza. Instituto Argentino de Investigaciones de las Zonas Áridas. Universidad Nacional de Cuyo. Instituto Argentino de Investigaciones de las Zonas Áridas; Argentina Fil: Egea, Angela Vanina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Argentino de Investigaciones de las Zonas Áridas. Provincia de Mendoza. Instituto Argentino de Investigaciones de las Zonas Áridas. Universidad Nacional de Cuyo. Instituto Argentino de Investigaciones de las Zonas Áridas; Argentina Fil: Schnittger, Leonhard. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina Fil: Cravero, Silvio Lorenzo Pedro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina Fil: Sosa Escudero, Miguel Angel. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina Fil: Allegretti, Liliana Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Argentino de Investigaciones de las Zonas Áridas. Provincia de Mendoza. Instituto Argentino de Investigaciones de las Zonas Áridas. Universidad Nacional de Cuyo. Instituto Argentino de Investigaciones de las Zonas Áridas; Argentina Fil: Arenas, Graciela Nora. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Cátedra de Microbiología; Argentina 2019-01-16T14:56:51Z 2019-01-16T14:56:51Z 2013-09 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://link.springer.com/article/10.1007/s12223-012-0219-1 http://hdl.handle.net/20.500.12123/4275 0015-5632 1874-9356 https://doi.org/10.1007/s12223-012-0219-1 eng info:eu-repo/semantics/restrictedAccess application/pdf Springer Folia Microbiologica 58 (5) : 367–373 (September 2013)
spellingShingle Caprinos
Razas (animales)
Digestión Ruminal
Forrajes
Alimentación de los Animales
Goats
Breeds (animals)
Rumen Digestion
Forage
Animal Feeding
Raza Criolla
Pseudobutyrivibrio ruminis
Pseudobutyrivibrio xylanivorans
Grilli, Diego Javier
Ceron Cucchi, Maria Esperanza
Paez Lama, Sebastián Antonio
Egea, Angela Vanina
Schnittger, Leonhard
Cravero, Silvio Lorenzo Pedro
Sosa Escudero, Miguel Angel
Allegretti, Liliana Inés
Arenas, Graciela Nora
Isolation of Pseudobutyrivibrio ruminis and Pseudobutyrivibrio xylanivorans from rumen of Creole goats fed native forage diet
title Isolation of Pseudobutyrivibrio ruminis and Pseudobutyrivibrio xylanivorans from rumen of Creole goats fed native forage diet
title_full Isolation of Pseudobutyrivibrio ruminis and Pseudobutyrivibrio xylanivorans from rumen of Creole goats fed native forage diet
title_fullStr Isolation of Pseudobutyrivibrio ruminis and Pseudobutyrivibrio xylanivorans from rumen of Creole goats fed native forage diet
title_full_unstemmed Isolation of Pseudobutyrivibrio ruminis and Pseudobutyrivibrio xylanivorans from rumen of Creole goats fed native forage diet
title_short Isolation of Pseudobutyrivibrio ruminis and Pseudobutyrivibrio xylanivorans from rumen of Creole goats fed native forage diet
title_sort isolation of pseudobutyrivibrio ruminis and pseudobutyrivibrio xylanivorans from rumen of creole goats fed native forage diet
topic Caprinos
Razas (animales)
Digestión Ruminal
Forrajes
Alimentación de los Animales
Goats
Breeds (animals)
Rumen Digestion
Forage
Animal Feeding
Raza Criolla
Pseudobutyrivibrio ruminis
Pseudobutyrivibrio xylanivorans
url https://link.springer.com/article/10.1007/s12223-012-0219-1
http://hdl.handle.net/20.500.12123/4275
https://doi.org/10.1007/s12223-012-0219-1
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