Genetic analysis of the 3′ untranslated region of the bovine SLC11A1 gene reveals novel polymorphisms
Polymorphisms in microsatellites at the 3′ untranslated region (3′UTR) of the SLC11A1 (solute carrier family 11 member A1) gene have been associated with natural resistance to Brucella abortus and Mycobacterium bovis infection in livestock species. Here, we carried out an individual genetic analysis...
| Autores principales: | , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Springer
2019
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| Materias: | |
| Acceso en línea: | https://link.springer.com/article/10.1007/s11033-012-2091-0 http://hdl.handle.net/20.500.12123/4274 https://doi.org/10.1007/s11033-012-2091-0 |
| _version_ | 1855483400618508288 |
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| author | Hasenauer, Flavia Carolina Caffaro, María Eugenia Czibener, Cecilia Comerci, Diego José Poli, Mario Andres Rossetti, Carlos Alberto |
| author_browse | Caffaro, María Eugenia Comerci, Diego José Czibener, Cecilia Hasenauer, Flavia Carolina Poli, Mario Andres Rossetti, Carlos Alberto |
| author_facet | Hasenauer, Flavia Carolina Caffaro, María Eugenia Czibener, Cecilia Comerci, Diego José Poli, Mario Andres Rossetti, Carlos Alberto |
| author_sort | Hasenauer, Flavia Carolina |
| collection | INTA Digital |
| description | Polymorphisms in microsatellites at the 3′ untranslated region (3′UTR) of the SLC11A1 (solute carrier family 11 member A1) gene have been associated with natural resistance to Brucella abortus and Mycobacterium bovis infection in livestock species. Here, we carried out an individual genetic analysis of the two microsatellites present at the 3′UTR SLC11A1 gene in 254 Bos taurus purebred, 125 B. indicus purebred and 54 B. taurus × B. indicus crossbred cattle. The genotyping by capillary electrophoresis showed the presence of four alleles (157, 159, 161 and 163) for the first microsatellite (MS1) and six alleles (175, 177, 179, 181, 183 and 185) for the second microsatellite (MS2). The alleles 159 and 175 were the most frequent in all breeds analyzed. B. taurus showed the most homogeneous haplotype and genotype for both microsatellites, whereas B. indicus showed the most heterogeneous haplotype and genotype. Two novel variants (alleles 161 and 163) within the MS1 are reported as well as novel variants in MS2 in Holstein breed. The knowledge of the polymorphisms distribution in both microsatellites at the 3′UTR of the SLC11A1 gene in cattle breeds is useful for future experimental design to evaluate the association between reported genotypes and natural resistance to pathogens infection. |
| format | Artículo |
| id | INTA4274 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA42742019-01-16T14:28:14Z Genetic analysis of the 3′ untranslated region of the bovine SLC11A1 gene reveals novel polymorphisms Hasenauer, Flavia Carolina Caffaro, María Eugenia Czibener, Cecilia Comerci, Diego José Poli, Mario Andres Rossetti, Carlos Alberto Genética Genes Ganado Bovino Polimorfismo Microsatélites Resistencia Genética Genetics Cattle Polymorphism Microsatellites Genetic Resistance Polymorphisms in microsatellites at the 3′ untranslated region (3′UTR) of the SLC11A1 (solute carrier family 11 member A1) gene have been associated with natural resistance to Brucella abortus and Mycobacterium bovis infection in livestock species. Here, we carried out an individual genetic analysis of the two microsatellites present at the 3′UTR SLC11A1 gene in 254 Bos taurus purebred, 125 B. indicus purebred and 54 B. taurus × B. indicus crossbred cattle. The genotyping by capillary electrophoresis showed the presence of four alleles (157, 159, 161 and 163) for the first microsatellite (MS1) and six alleles (175, 177, 179, 181, 183 and 185) for the second microsatellite (MS2). The alleles 159 and 175 were the most frequent in all breeds analyzed. B. taurus showed the most homogeneous haplotype and genotype for both microsatellites, whereas B. indicus showed the most heterogeneous haplotype and genotype. Two novel variants (alleles 161 and 163) within the MS1 are reported as well as novel variants in MS2 in Holstein breed. The knowledge of the polymorphisms distribution in both microsatellites at the 3′UTR of the SLC11A1 gene in cattle breeds is useful for future experimental design to evaluate the association between reported genotypes and natural resistance to pathogens infection. Instituto de Patobiología Fil: Hasenauer, Flavia Carolina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Caffaro, María Eugenia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Czibener, Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina Fil: Comerci, Diego José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina Fil: Poli, Mario Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Rossetti, Carlos Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina 2019-01-16T14:26:41Z 2019-01-16T14:26:41Z 2013-01 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://link.springer.com/article/10.1007/s11033-012-2091-0 http://hdl.handle.net/20.500.12123/4274 0301-4851 1573-4978 https://doi.org/10.1007/s11033-012-2091-0 eng info:eu-repo/semantics/restrictedAccess application/pdf Springer Molecular Biology Reports 40 (1) : 545–552 (January 2013) |
| spellingShingle | Genética Genes Ganado Bovino Polimorfismo Microsatélites Resistencia Genética Genetics Cattle Polymorphism Microsatellites Genetic Resistance Hasenauer, Flavia Carolina Caffaro, María Eugenia Czibener, Cecilia Comerci, Diego José Poli, Mario Andres Rossetti, Carlos Alberto Genetic analysis of the 3′ untranslated region of the bovine SLC11A1 gene reveals novel polymorphisms |
| title | Genetic analysis of the 3′ untranslated region of the bovine SLC11A1 gene reveals novel polymorphisms |
| title_full | Genetic analysis of the 3′ untranslated region of the bovine SLC11A1 gene reveals novel polymorphisms |
| title_fullStr | Genetic analysis of the 3′ untranslated region of the bovine SLC11A1 gene reveals novel polymorphisms |
| title_full_unstemmed | Genetic analysis of the 3′ untranslated region of the bovine SLC11A1 gene reveals novel polymorphisms |
| title_short | Genetic analysis of the 3′ untranslated region of the bovine SLC11A1 gene reveals novel polymorphisms |
| title_sort | genetic analysis of the 3 untranslated region of the bovine slc11a1 gene reveals novel polymorphisms |
| topic | Genética Genes Ganado Bovino Polimorfismo Microsatélites Resistencia Genética Genetics Cattle Polymorphism Microsatellites Genetic Resistance |
| url | https://link.springer.com/article/10.1007/s11033-012-2091-0 http://hdl.handle.net/20.500.12123/4274 https://doi.org/10.1007/s11033-012-2091-0 |
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