Characterization of Cellulolytic Activities of Environmental Bacterial Consortia from an Argentinian Native Forest
Cellulolytic activities of three bacterial consortia derived from a forest soil sample from Chaco region, Argentina, were characterized. The phylogenetic analysis of consortia revealed two main highly supported groups including Achromobacter and Pseudomonas genera. All three consortia presented cell...
| Autores principales: | , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
Springer
2019
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| Materias: | |
| Acceso en línea: | https://link.springer.com/article/10.1007%2Fs00284-013-0345-2 http://hdl.handle.net/20.500.12123/4272 https://doi.org/10.1007/s00284-013-0345-2 |
| _version_ | 1855035291628208128 |
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| author | Romano, Nelson Gaston Gioffre, Andrea Sede, Silvana Mabel Campos, Eleonora Cataldi, Angel Adrian Talia, Paola Mónica |
| author_browse | Campos, Eleonora Cataldi, Angel Adrian Gioffre, Andrea Romano, Nelson Gaston Sede, Silvana Mabel Talia, Paola Mónica |
| author_facet | Romano, Nelson Gaston Gioffre, Andrea Sede, Silvana Mabel Campos, Eleonora Cataldi, Angel Adrian Talia, Paola Mónica |
| author_sort | Romano, Nelson Gaston |
| collection | INTA Digital |
| description | Cellulolytic activities of three bacterial consortia derived from a forest soil sample from Chaco region, Argentina, were characterized. The phylogenetic analysis of consortia revealed two main highly supported groups including Achromobacter and Pseudomonas genera. All three consortia presented cellulolytic activity. The carboxymethylcellulase (CMCase) and total cellulase activities were studied both quantitatively and qualitatively and optimal enzymatic conditions were characterized and compared among the three consortia. Thermal and pH stability were analyzed. Based on its cellulolytic activity, one consortium was selected for further characterization by zymography. We detected a specific protein of 55 kDa with CMCase activity. In this study, we have shown that these consortia encode for cellulolytic enzymes. These enzymes could be useful for lignocellulosic biomass degradation into simple components and for different industrial applications. |
| format | info:ar-repo/semantics/artículo |
| id | INTA4272 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA42722019-01-15T17:56:51Z Characterization of Cellulolytic Activities of Environmental Bacterial Consortia from an Argentinian Native Forest Romano, Nelson Gaston Gioffre, Andrea Sede, Silvana Mabel Campos, Eleonora Cataldi, Angel Adrian Talia, Paola Mónica Bosques Bosque Primario Pseudomonas Celulasa Actividad Enzimática Forests Primary Forests Cellulase Enzyme Activity Bosques Nativos Argentina Cellulolytic activities of three bacterial consortia derived from a forest soil sample from Chaco region, Argentina, were characterized. The phylogenetic analysis of consortia revealed two main highly supported groups including Achromobacter and Pseudomonas genera. All three consortia presented cellulolytic activity. The carboxymethylcellulase (CMCase) and total cellulase activities were studied both quantitatively and qualitatively and optimal enzymatic conditions were characterized and compared among the three consortia. Thermal and pH stability were analyzed. Based on its cellulolytic activity, one consortium was selected for further characterization by zymography. We detected a specific protein of 55 kDa with CMCase activity. In this study, we have shown that these consortia encode for cellulolytic enzymes. These enzymes could be useful for lignocellulosic biomass degradation into simple components and for different industrial applications. Instituto de Biotecnología Fil: Romano, Nelson Gaston. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Fil: Gioffre, Andrea Karina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Sede, Silvana Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica Darwinion. Academia Nacional de Ciencias Exactas, Físicas y Naturales. Instituto de Botánica Darwinion; Argentina Fil: Campos, Eleonora. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Talia, Paola Mónica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina 2019-01-15T17:55:21Z 2019-01-15T17:55:21Z 2013-08 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://link.springer.com/article/10.1007%2Fs00284-013-0345-2 http://hdl.handle.net/20.500.12123/4272 0343-8651 1432-0991 https://doi.org/10.1007/s00284-013-0345-2 eng info:eu-repo/semantics/restrictedAccess application/pdf Springer Current Microbiology 67 (2) : 138–147 (August 2013) |
| spellingShingle | Bosques Bosque Primario Pseudomonas Celulasa Actividad Enzimática Forests Primary Forests Cellulase Enzyme Activity Bosques Nativos Argentina Romano, Nelson Gaston Gioffre, Andrea Sede, Silvana Mabel Campos, Eleonora Cataldi, Angel Adrian Talia, Paola Mónica Characterization of Cellulolytic Activities of Environmental Bacterial Consortia from an Argentinian Native Forest |
| title | Characterization of Cellulolytic Activities of Environmental Bacterial Consortia from an Argentinian Native Forest |
| title_full | Characterization of Cellulolytic Activities of Environmental Bacterial Consortia from an Argentinian Native Forest |
| title_fullStr | Characterization of Cellulolytic Activities of Environmental Bacterial Consortia from an Argentinian Native Forest |
| title_full_unstemmed | Characterization of Cellulolytic Activities of Environmental Bacterial Consortia from an Argentinian Native Forest |
| title_short | Characterization of Cellulolytic Activities of Environmental Bacterial Consortia from an Argentinian Native Forest |
| title_sort | characterization of cellulolytic activities of environmental bacterial consortia from an argentinian native forest |
| topic | Bosques Bosque Primario Pseudomonas Celulasa Actividad Enzimática Forests Primary Forests Cellulase Enzyme Activity Bosques Nativos Argentina |
| url | https://link.springer.com/article/10.1007%2Fs00284-013-0345-2 http://hdl.handle.net/20.500.12123/4272 https://doi.org/10.1007/s00284-013-0345-2 |
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