Molecular Diversity in Selected Banana Clones (Musa AAA “Cavendish”) : adapted to the Subtropical Environment of Formosa Province (Argentina)

This study employed Amplified Fragment Length Polymorphism to investigate the genetic variation in local banana plants from farmer’s fields. Forty-four rhizomes (selected from a total of 860 plants for being stable for production) were used as plant material and 6 primer combinations selected in a p...

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Autores principales: Ermini, José Luis, Tenaglia, Gerardo Carlos, Pratta, Guillermo Raúl
Formato: info:ar-repo/semantics/artículo
Lenguaje:Inglés
Publicado: Scientific Research Publishing 2019
Materias:
Acceso en línea:http://hdl.handle.net/20.500.12123/4268
https://www.scirp.org/journal/PaperInformation.aspx?PaperID=88868
https://doi.org/10.4236/ajps.2018.912181
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author Ermini, José Luis
Tenaglia, Gerardo Carlos
Pratta, Guillermo Raúl
author_browse Ermini, José Luis
Pratta, Guillermo Raúl
Tenaglia, Gerardo Carlos
author_facet Ermini, José Luis
Tenaglia, Gerardo Carlos
Pratta, Guillermo Raúl
author_sort Ermini, José Luis
collection INTA Digital
description This study employed Amplified Fragment Length Polymorphism to investigate the genetic variation in local banana plants from farmer’s fields. Forty-four rhizomes (selected from a total of 860 plants for being stable for production) were used as plant material and 6 primer combinations selected in a previous report were used. Polymorphic fragments present in a given genotype were assigned 1 and those absent were assigned 0. The matrix generated was analyzed by univariate and multivariate analysis. A total 540 bands were scored, of which 100% were polymorphic. The number of the pattern duplicated bands was 23 and twenty-one amplicons were exclusive to banana plants collected in the same farmer’s field. Hierarchical clustering and principal coordinates analyses showed differences between genotypes. The cophenetic correlation of the cluster was 0.63 while the 18% of the total molecular variation was explained by the two principal coordinates. These analyses evidence genetic diversity of the crop according to farmer’s field. In agreement to this observation, analysis of molecular variance revealed that 8.9% of the variation was found among farmer populations and 91.1% within farmer populations. In conclusion, introduction of banana into the subtropical zone was associated with a broader genetic variation in order to increase the genetic homeostasis necessary for adapting the crop to the suboptimal environment of Northeastern Argentinean Formosa Province.
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institution Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina)
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publishDateRange 2019
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spelling INTA42682024-01-10T11:15:27Z Molecular Diversity in Selected Banana Clones (Musa AAA “Cavendish”) : adapted to the Subtropical Environment of Formosa Province (Argentina) Ermini, José Luis Tenaglia, Gerardo Carlos Pratta, Guillermo Raúl Plant Genetic Resources Subtropical Zones Bananas Multivariate Analysis Musa Banano Recursos Genéticos Vegetales Zona Subtropical Clones Análisis Multivariante Autotriploidy Molecular Characterization Formosa, Argentina Argentina Caracterización Molecular Diversidad Molecular This study employed Amplified Fragment Length Polymorphism to investigate the genetic variation in local banana plants from farmer’s fields. Forty-four rhizomes (selected from a total of 860 plants for being stable for production) were used as plant material and 6 primer combinations selected in a previous report were used. Polymorphic fragments present in a given genotype were assigned 1 and those absent were assigned 0. The matrix generated was analyzed by univariate and multivariate analysis. A total 540 bands were scored, of which 100% were polymorphic. The number of the pattern duplicated bands was 23 and twenty-one amplicons were exclusive to banana plants collected in the same farmer’s field. Hierarchical clustering and principal coordinates analyses showed differences between genotypes. The cophenetic correlation of the cluster was 0.63 while the 18% of the total molecular variation was explained by the two principal coordinates. These analyses evidence genetic diversity of the crop according to farmer’s field. In agreement to this observation, analysis of molecular variance revealed that 8.9% of the variation was found among farmer populations and 91.1% within farmer populations. In conclusion, introduction of banana into the subtropical zone was associated with a broader genetic variation in order to increase the genetic homeostasis necessary for adapting the crop to the suboptimal environment of Northeastern Argentinean Formosa Province. Investigación y Desarrollo Tecnológico para la Agricultura Familiar Región NEA Fil: Ermini, José Luis. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Departamento de Biología. Cátedra de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina Fil: Tenaglia, Gerardo Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación y Desarrollo Tecnológico para la Agricultura Familiar Región NEA; Argentina Fil: Pratta, Guillermo Raúl. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Departamento de Biología. Cátedra de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Departamento de Biología. Cátedra de Genética; Argentina 2019-01-15T14:07:30Z 2019-01-15T14:07:30Z 2018-11-30 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/4268 https://www.scirp.org/journal/PaperInformation.aspx?PaperID=88868 2158-2742 2158-2750 https://doi.org/10.4236/ajps.2018.912181 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Scientific Research Publishing American Journal of Plant Sciences /Scientific Research Publishing 9 (12) : 2504-2513 (2018).
spellingShingle Plant Genetic Resources
Subtropical Zones
Bananas
Multivariate Analysis
Musa
Banano
Recursos Genéticos Vegetales
Zona Subtropical
Clones
Análisis Multivariante
Autotriploidy
Molecular Characterization
Formosa, Argentina
Argentina
Caracterización Molecular
Diversidad Molecular
Ermini, José Luis
Tenaglia, Gerardo Carlos
Pratta, Guillermo Raúl
Molecular Diversity in Selected Banana Clones (Musa AAA “Cavendish”) : adapted to the Subtropical Environment of Formosa Province (Argentina)
title Molecular Diversity in Selected Banana Clones (Musa AAA “Cavendish”) : adapted to the Subtropical Environment of Formosa Province (Argentina)
title_full Molecular Diversity in Selected Banana Clones (Musa AAA “Cavendish”) : adapted to the Subtropical Environment of Formosa Province (Argentina)
title_fullStr Molecular Diversity in Selected Banana Clones (Musa AAA “Cavendish”) : adapted to the Subtropical Environment of Formosa Province (Argentina)
title_full_unstemmed Molecular Diversity in Selected Banana Clones (Musa AAA “Cavendish”) : adapted to the Subtropical Environment of Formosa Province (Argentina)
title_short Molecular Diversity in Selected Banana Clones (Musa AAA “Cavendish”) : adapted to the Subtropical Environment of Formosa Province (Argentina)
title_sort molecular diversity in selected banana clones musa aaa cavendish adapted to the subtropical environment of formosa province argentina
topic Plant Genetic Resources
Subtropical Zones
Bananas
Multivariate Analysis
Musa
Banano
Recursos Genéticos Vegetales
Zona Subtropical
Clones
Análisis Multivariante
Autotriploidy
Molecular Characterization
Formosa, Argentina
Argentina
Caracterización Molecular
Diversidad Molecular
url http://hdl.handle.net/20.500.12123/4268
https://www.scirp.org/journal/PaperInformation.aspx?PaperID=88868
https://doi.org/10.4236/ajps.2018.912181
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