Population genetics structure of glyphosate‐resistant Johnsongrass (Sorghum halepense L. Pers) does not support a single origin of the resistance
Single sequence repeats (SSR) developed for Sorghum bicolor were used to characterize the genetic distance of 46 different Sorghum halepense (Johnsongrass) accessions from Argentina some of which have evolved toward glyphosate resistance. Since Johnsongrass is an allotetraploid and only one subgenom...
| Autores principales: | , , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Wiley
2019
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| Materias: | |
| Acceso en línea: | https://onlinelibrary.wiley.com/doi/full/10.1002/ece3.671 http://hdl.handle.net/20.500.12123/4266 https://doi.org/10.1002/ece3.671 |
| _version_ | 1855483399045644288 |
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| author | Fernandez, Luis German De Haro, Luis Alejandro Distefano, Ana Julia Martinez, Maria Carolina Lia, Veronica Viviana Papa, Juan Carlos Olea, Ignacio Tosto, Daniela Sandra Hopp, Horacio Esteban |
| author_browse | De Haro, Luis Alejandro Distefano, Ana Julia Fernandez, Luis German Hopp, Horacio Esteban Lia, Veronica Viviana Martinez, Maria Carolina Olea, Ignacio Papa, Juan Carlos Tosto, Daniela Sandra |
| author_facet | Fernandez, Luis German De Haro, Luis Alejandro Distefano, Ana Julia Martinez, Maria Carolina Lia, Veronica Viviana Papa, Juan Carlos Olea, Ignacio Tosto, Daniela Sandra Hopp, Horacio Esteban |
| author_sort | Fernandez, Luis German |
| collection | INTA Digital |
| description | Single sequence repeats (SSR) developed for Sorghum bicolor were used to characterize the genetic distance of 46 different Sorghum halepense (Johnsongrass) accessions from Argentina some of which have evolved toward glyphosate resistance. Since Johnsongrass is an allotetraploid and only one subgenome is homologous to cultivated sorghum, some SSR loci amplified up to two alleles while others (presumably more conserved loci) amplified up to four alleles. Twelve SSR providing information of 24 loci representative of Johnsongrass genome were selected for genetic distance characterization. All of them were highly polymorphic, which was evidenced by the number of different alleles found in the samples studied, in some of them up to 20. UPGMA and Mantel analysis showed that Johnsongrass glyphosate‐resistant accessions that belong to different geographic regions do not share similar genetic backgrounds. In contrast, they show closer similarity to their neighboring susceptible counterparts. Discriminant Analysis of Principal Components using the clusters identified by K‐means support the lack of a clear pattern of association among samples and resistance status or province of origin. Consequently, these results do not support a single genetic origin of glyphosate resistance. Nucleotide sequencing of the 5‐enolpyruvylshikimate‐3‐phosphate synthase (EPSPS) encoding gene from glyphosate‐resistant and susceptible accessions collected from different geographic origins showed that none presented expected mutations in aminoacid positions 101 and 106 which are diagnostic of target‐site resistance mechanism. |
| format | Artículo |
| id | INTA4266 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | INTA42662019-01-15T13:25:46Z Population genetics structure of glyphosate‐resistant Johnsongrass (Sorghum halepense L. Pers) does not support a single origin of the resistance Fernandez, Luis German De Haro, Luis Alejandro Distefano, Ana Julia Martinez, Maria Carolina Lia, Veronica Viviana Papa, Juan Carlos Olea, Ignacio Tosto, Daniela Sandra Hopp, Horacio Esteban Sorghum halepense Genética Resistencia a los Herbicidas Glifosato Malezas Genetics Resistance to Herbicides Glyphosate Weeds Sorgo de Alepo Johnsongrass Single sequence repeats (SSR) developed for Sorghum bicolor were used to characterize the genetic distance of 46 different Sorghum halepense (Johnsongrass) accessions from Argentina some of which have evolved toward glyphosate resistance. Since Johnsongrass is an allotetraploid and only one subgenome is homologous to cultivated sorghum, some SSR loci amplified up to two alleles while others (presumably more conserved loci) amplified up to four alleles. Twelve SSR providing information of 24 loci representative of Johnsongrass genome were selected for genetic distance characterization. All of them were highly polymorphic, which was evidenced by the number of different alleles found in the samples studied, in some of them up to 20. UPGMA and Mantel analysis showed that Johnsongrass glyphosate‐resistant accessions that belong to different geographic regions do not share similar genetic backgrounds. In contrast, they show closer similarity to their neighboring susceptible counterparts. Discriminant Analysis of Principal Components using the clusters identified by K‐means support the lack of a clear pattern of association among samples and resistance status or province of origin. Consequently, these results do not support a single genetic origin of glyphosate resistance. Nucleotide sequencing of the 5‐enolpyruvylshikimate‐3‐phosphate synthase (EPSPS) encoding gene from glyphosate‐resistant and susceptible accessions collected from different geographic origins showed that none presented expected mutations in aminoacid positions 101 and 106 which are diagnostic of target‐site resistance mechanism. Instituto de Biotecnología Fil: Fernandez, Luis German. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: De Haro, Luis Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Distefano, Ana Julia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina Fil: Martinez, Maria Carolina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina Fil: Lia, Veronica Viviana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina Fil: Papa, Juan Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Oliveros; Argentina Fil: Olea, Ignacio. Estación Experimental Agroindustrial Obispo Colombres. Sección Malezas; Argentina Fil: Tosto, Daniela Sandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina Fil: Hopp, Horacio Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina 2019-01-15T13:24:37Z 2019-01-15T13:24:37Z 2013-09 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://onlinelibrary.wiley.com/doi/full/10.1002/ece3.671 http://hdl.handle.net/20.500.12123/4266 2045-7758 https://doi.org/10.1002/ece3.671 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Wiley Ecology and Evolution 3 (10) : 3388-3400 (September 2013) |
| spellingShingle | Sorghum halepense Genética Resistencia a los Herbicidas Glifosato Malezas Genetics Resistance to Herbicides Glyphosate Weeds Sorgo de Alepo Johnsongrass Fernandez, Luis German De Haro, Luis Alejandro Distefano, Ana Julia Martinez, Maria Carolina Lia, Veronica Viviana Papa, Juan Carlos Olea, Ignacio Tosto, Daniela Sandra Hopp, Horacio Esteban Population genetics structure of glyphosate‐resistant Johnsongrass (Sorghum halepense L. Pers) does not support a single origin of the resistance |
| title | Population genetics structure of glyphosate‐resistant Johnsongrass (Sorghum halepense L. Pers) does not support a single origin of the resistance |
| title_full | Population genetics structure of glyphosate‐resistant Johnsongrass (Sorghum halepense L. Pers) does not support a single origin of the resistance |
| title_fullStr | Population genetics structure of glyphosate‐resistant Johnsongrass (Sorghum halepense L. Pers) does not support a single origin of the resistance |
| title_full_unstemmed | Population genetics structure of glyphosate‐resistant Johnsongrass (Sorghum halepense L. Pers) does not support a single origin of the resistance |
| title_short | Population genetics structure of glyphosate‐resistant Johnsongrass (Sorghum halepense L. Pers) does not support a single origin of the resistance |
| title_sort | population genetics structure of glyphosate resistant johnsongrass sorghum halepense l pers does not support a single origin of the resistance |
| topic | Sorghum halepense Genética Resistencia a los Herbicidas Glifosato Malezas Genetics Resistance to Herbicides Glyphosate Weeds Sorgo de Alepo Johnsongrass |
| url | https://onlinelibrary.wiley.com/doi/full/10.1002/ece3.671 http://hdl.handle.net/20.500.12123/4266 https://doi.org/10.1002/ece3.671 |
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