An analysis of the genomic variability of the phytopathogenic mollicute Spiroplasma kunkelii
Corn stunt disease has become a factor limiting maize production in some areas of the Americas in recent years. Although resistant maize genotypes have been developed in the past, this resistance has been unstable over time or in some geographical locations. To better understand disease components t...
| Autores principales: | , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
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American Phytopathological Society
2019
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/4264 https://apsjournals.apsnet.org/doi/10.1094/PHYTO-07-12-0158-R https://doi.org/10.1094/PHYTO-07-12-0158-R |
| _version_ | 1855035290119307264 |
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| author | Carpane, Pablo D. Melcher, Ulrich Wayadande, Astri Gimenez Pecci, Maria De La Paz Laguna, Irma Graciela Dolezal, William Fletcher, Jacqueline |
| author_browse | Carpane, Pablo D. Dolezal, William Fletcher, Jacqueline Gimenez Pecci, Maria De La Paz Laguna, Irma Graciela Melcher, Ulrich Wayadande, Astri |
| author_facet | Carpane, Pablo D. Melcher, Ulrich Wayadande, Astri Gimenez Pecci, Maria De La Paz Laguna, Irma Graciela Dolezal, William Fletcher, Jacqueline |
| author_sort | Carpane, Pablo D. |
| collection | INTA Digital |
| description | Corn stunt disease has become a factor limiting maize production in some areas of the Americas in recent years. Although resistant maize genotypes have been developed in the past, this resistance has been unstable over time or in some geographical locations. To better understand disease components that could affect the stability of host resistance, we assessed the genome variability of the etiologic agent, Spiroplasma kunkelii. Isolates were obtained from a number of areas, and characterized molecularly by amplification of several regions of the spiroplasma chromosome and sequencing of specific gene fragments. The degree of polymorphism between isolates of different geographic origins was low, and the level of genomic variability was similar within isolates of different countries. Polymorphism among isolates was found in viral insertions and in the sequence of Skarp, a gene that encodes a membrane protein implicated in attachment to insect cells. The results suggest that the genome composition of this species is highly conserved among isolates. Hence, it is unlikely that the instability of maize resistance is due to generation of new pathotypes of S. kunkelii. Instead, other components of this complex pathosystem could account for the breakdown of resistance. |
| format | info:ar-repo/semantics/artículo |
| id | INTA4264 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | American Phytopathological Society |
| publisherStr | American Phytopathological Society |
| record_format | dspace |
| spelling | INTA42642025-09-24T10:21:50Z An analysis of the genomic variability of the phytopathogenic mollicute Spiroplasma kunkelii Carpane, Pablo D. Melcher, Ulrich Wayadande, Astri Gimenez Pecci, Maria De La Paz Laguna, Irma Graciela Dolezal, William Fletcher, Jacqueline Enfermedades de las Plantas Spiroplasma Variación Genética Maíz Polimorfismo Plant Diseases Genetic Variation Maize Polymorphism Spiroplasma kunkelii Corn stunt disease has become a factor limiting maize production in some areas of the Americas in recent years. Although resistant maize genotypes have been developed in the past, this resistance has been unstable over time or in some geographical locations. To better understand disease components that could affect the stability of host resistance, we assessed the genome variability of the etiologic agent, Spiroplasma kunkelii. Isolates were obtained from a number of areas, and characterized molecularly by amplification of several regions of the spiroplasma chromosome and sequencing of specific gene fragments. The degree of polymorphism between isolates of different geographic origins was low, and the level of genomic variability was similar within isolates of different countries. Polymorphism among isolates was found in viral insertions and in the sequence of Skarp, a gene that encodes a membrane protein implicated in attachment to insect cells. The results suggest that the genome composition of this species is highly conserved among isolates. Hence, it is unlikely that the instability of maize resistance is due to generation of new pathotypes of S. kunkelii. Instead, other components of this complex pathosystem could account for the breakdown of resistance. Instituto de Patología Vegetal Fil: Carpane, Pablo. Oklahoma State University. Department of Entomology and Plant Pathology; Estados Unidos Fil: Melcher, Ulrich. Oklahoma State University. Department of Biochemistry and Molecular Biology; Estados Unidos Fil: Wayadande, Astri. Oklahoma State University. Department of Entomology and Plant Pathology; Estados Unidos Fil: Giménez Pecci, María de la Paz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Laguna, Irma Graciela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Dolezal, William. Pioneer Hi-Bred International; Estados Unidos Fil: Fletcher, Jacqueline. Oklahoma State University. Department of Entomology and Plant Pathology; Estados Unidos 2019-01-14T17:30:09Z 2019-01-14T17:30:09Z 2013-02 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/4264 https://apsjournals.apsnet.org/doi/10.1094/PHYTO-07-12-0158-R 0031-949X 1943-7684 https://doi.org/10.1094/PHYTO-07-12-0158-R eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf American Phytopathological Society Phytopathology 103 (2) : 129-134 (February 2013) |
| spellingShingle | Enfermedades de las Plantas Spiroplasma Variación Genética Maíz Polimorfismo Plant Diseases Genetic Variation Maize Polymorphism Spiroplasma kunkelii Carpane, Pablo D. Melcher, Ulrich Wayadande, Astri Gimenez Pecci, Maria De La Paz Laguna, Irma Graciela Dolezal, William Fletcher, Jacqueline An analysis of the genomic variability of the phytopathogenic mollicute Spiroplasma kunkelii |
| title | An analysis of the genomic variability of the phytopathogenic mollicute Spiroplasma kunkelii |
| title_full | An analysis of the genomic variability of the phytopathogenic mollicute Spiroplasma kunkelii |
| title_fullStr | An analysis of the genomic variability of the phytopathogenic mollicute Spiroplasma kunkelii |
| title_full_unstemmed | An analysis of the genomic variability of the phytopathogenic mollicute Spiroplasma kunkelii |
| title_short | An analysis of the genomic variability of the phytopathogenic mollicute Spiroplasma kunkelii |
| title_sort | analysis of the genomic variability of the phytopathogenic mollicute spiroplasma kunkelii |
| topic | Enfermedades de las Plantas Spiroplasma Variación Genética Maíz Polimorfismo Plant Diseases Genetic Variation Maize Polymorphism Spiroplasma kunkelii |
| url | http://hdl.handle.net/20.500.12123/4264 https://apsjournals.apsnet.org/doi/10.1094/PHYTO-07-12-0158-R https://doi.org/10.1094/PHYTO-07-12-0158-R |
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