Validation of models to estimate the fumigant and larvicidal activity of Eucalyptus essential oils against Aedes aegypti (Diptera: Culicidae)

The aim of this work is to validate the pre-existing models that relate the larvicidal and adulticidal activities of the Eucalyptus essential oils on Aedes aegypti. Previous works at our laboratory described that the larvicidal activity of Eucalyptus essential oils can be estimated from the relative...

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Main Authors: Lucia, Alejandro, Juan, Laura Wilma, Zerba, Eduardo Nicolás, Harrand, Leonel, Marco, Martin Alberto, Masuh, Hector Mario
Format: info:ar-repo/semantics/artículo
Language:Inglés
Published: Springer 2019
Subjects:
Online Access:https://link.springer.com/article/10.1007%2Fs00436-011-2685-9
http://hdl.handle.net/20.500.12123/4263
https://doi.org/10.1007/s00436-011-2685-9
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author Lucia, Alejandro
Juan, Laura Wilma
Zerba, Eduardo Nicolás
Harrand, Leonel
Marco, Martin Alberto
Masuh, Hector Mario
author_browse Harrand, Leonel
Juan, Laura Wilma
Lucia, Alejandro
Marco, Martin Alberto
Masuh, Hector Mario
Zerba, Eduardo Nicolás
author_facet Lucia, Alejandro
Juan, Laura Wilma
Zerba, Eduardo Nicolás
Harrand, Leonel
Marco, Martin Alberto
Masuh, Hector Mario
author_sort Lucia, Alejandro
collection INTA Digital
description The aim of this work is to validate the pre-existing models that relate the larvicidal and adulticidal activities of the Eucalyptus essential oils on Aedes aegypti. Previous works at our laboratory described that the larvicidal activity of Eucalyptus essential oils can be estimated from the relative concentration of two main components (p-cymene and 1,8-cineole) and that the adulticidal effectiveness can be explained, to a great extent, by the presence of large amounts of the component 1,8-cineole in it. In general, the results show that the higher adulticidal effect of essential oils the lower their larvicidal activity. Fresh leaves was harvested and distilled. Once the essential oil was obtained, the chemical composition was analysed, evaluating the biological activity of 15 species of the genus Eucalyptus (Eucalyptus badjensis Beuzev and Welch, Eucalyptus badjensis × nitens, Eucalyptus benthamii var Benthamii Maiden and Cambage, Eucalyptus benthamii var dorrigoensis Maiden and Cambage, Eucalyptus botryoides Smith, Eucalyptus dalrympleana Maiden, Eucalyptus fastigata Deane and Maiden, Eucalyptus nobilis L.A.S. Johnson and K.D.Hill, Eucalyptus polybractea R. Baker, Eucalyptus radiata ssp radiata Sieber ex Spreng, Eucalyptus resinifera Smith, Eucalyptus robertsonii Blakely, Eucalyptus robusta Smith, Eucalyptus rubida Deane and Maiden, Eucalyptus smithii R. Baker). Essential oils of these plant species were used for the validation of equations from preexistent models, in which observed and estimated values of the biological activity were compared. The regression analysis showed a strong validation of the models, re-stating the trends previously observed. The models were expressed as follows: A, fumigant activity [KT50(min) = 10.65–0.076 × 1,8-cineole (%)](p < 0.01; F, 397; R 2, 0.79); B, larval mortality (%)(40 ppm) = 103.85 + 0.482 × p-cymene (%) − 0.363 × α-pinene (%) − 1.07 × 1,8-cineole (%) (p < 0.01; F, 300; R 2, 0.90). These results confirmed the importance of the mayor components in the biological activity of Eucalyptus essential oils on A. aegypti. However, it is worth mentioning that two or three species differ in the data estimated by the models, and these biological activity results coincide with the presence of minor differential components in the essential oils. According to what was previously mentioned, it can be inferred that the model is able to estimate very closely the biological activity of essential oils of Eucalyptus on A. aegypti.
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spelling INTA42632019-01-14T17:00:38Z Validation of models to estimate the fumigant and larvicidal activity of Eucalyptus essential oils against Aedes aegypti (Diptera: Culicidae) Lucia, Alejandro Juan, Laura Wilma Zerba, Eduardo Nicolás Harrand, Leonel Marco, Martin Alberto Masuh, Hector Mario Aedes Aegypti Control de Plagas Aceites Esenciales Eucalyptus Larvicidas Pest Control Essential Oils Larvicides Mosquitos Fiebre Amarilla The aim of this work is to validate the pre-existing models that relate the larvicidal and adulticidal activities of the Eucalyptus essential oils on Aedes aegypti. Previous works at our laboratory described that the larvicidal activity of Eucalyptus essential oils can be estimated from the relative concentration of two main components (p-cymene and 1,8-cineole) and that the adulticidal effectiveness can be explained, to a great extent, by the presence of large amounts of the component 1,8-cineole in it. In general, the results show that the higher adulticidal effect of essential oils the lower their larvicidal activity. Fresh leaves was harvested and distilled. Once the essential oil was obtained, the chemical composition was analysed, evaluating the biological activity of 15 species of the genus Eucalyptus (Eucalyptus badjensis Beuzev and Welch, Eucalyptus badjensis × nitens, Eucalyptus benthamii var Benthamii Maiden and Cambage, Eucalyptus benthamii var dorrigoensis Maiden and Cambage, Eucalyptus botryoides Smith, Eucalyptus dalrympleana Maiden, Eucalyptus fastigata Deane and Maiden, Eucalyptus nobilis L.A.S. Johnson and K.D.Hill, Eucalyptus polybractea R. Baker, Eucalyptus radiata ssp radiata Sieber ex Spreng, Eucalyptus resinifera Smith, Eucalyptus robertsonii Blakely, Eucalyptus robusta Smith, Eucalyptus rubida Deane and Maiden, Eucalyptus smithii R. Baker). Essential oils of these plant species were used for the validation of equations from preexistent models, in which observed and estimated values of the biological activity were compared. The regression analysis showed a strong validation of the models, re-stating the trends previously observed. The models were expressed as follows: A, fumigant activity [KT50(min) = 10.65–0.076 × 1,8-cineole (%)](p < 0.01; F, 397; R 2, 0.79); B, larval mortality (%)(40 ppm) = 103.85 + 0.482 × p-cymene (%) − 0.363 × α-pinene (%) − 1.07 × 1,8-cineole (%) (p < 0.01; F, 300; R 2, 0.90). These results confirmed the importance of the mayor components in the biological activity of Eucalyptus essential oils on A. aegypti. However, it is worth mentioning that two or three species differ in the data estimated by the models, and these biological activity results coincide with the presence of minor differential components in the essential oils. According to what was previously mentioned, it can be inferred that the model is able to estimate very closely the biological activity of essential oils of Eucalyptus on A. aegypti. EEA Concordia Fil: Fil: Lucia, Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Investigación y Desarrollo Estratégico para la Defensa. Ministerio de Defensa. Unidad de Investigación y Desarrollo Estratégico para la Defensa; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Científicas y Técnicas para la Defensa. Centro de Investigación de Plagas e Insecticidas; Fil: Juan, Laura Wilma. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Investigación y Desarrollo Estratégico para la Defensa. Ministerio de Defensa. Unidad de Investigación y Desarrollo Estratégico para la Defensa; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Científicas y Técnicas para la Defensa. Centro de Investigación de Plagas e Insecticidas; Argentina Fil: Zerba, Eduardo Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Investigación y Desarrollo Estratégico para la Defensa. Ministerio de Defensa. Unidad de Investigación y Desarrollo Estratégico para la Defensa; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Científicas y Técnicas para la Defensa. Centro de Investigación de Plagas e Insecticidas; Argentina. Universidad Nacional de San Martín. Instituto de Investigación e Ingeniería Ambiental; Argentina Fil: Harrand, Leonel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina Fil: Marco, Martin Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina Fil: Masuh, Hector Mario. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Investigación y Desarrollo Estratégico para la Defensa. Ministerio de Defensa. Unidad de Investigación y Desarrollo Estratégico para la Defensa; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Científicas y Técnicas para la Defensa. Centro de Investigación de Plagas e Insecticidas; Argentina. Universidad Nacional de San Martín. Instituto de Investigación e Ingeniería Ambiental; Argentina 2019-01-14T16:58:28Z 2019-01-14T16:58:28Z 2012-05 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://link.springer.com/article/10.1007%2Fs00436-011-2685-9 http://hdl.handle.net/20.500.12123/4263 0932-0113 1432-1955 https://doi.org/10.1007/s00436-011-2685-9 eng info:eu-repo/semantics/restrictedAccess application/pdf Springer Parasitology Research 110 (5) : 1675–1686 (May 2012)
spellingShingle Aedes Aegypti
Control de Plagas
Aceites Esenciales
Eucalyptus
Larvicidas
Pest Control
Essential Oils
Larvicides
Mosquitos
Fiebre Amarilla
Lucia, Alejandro
Juan, Laura Wilma
Zerba, Eduardo Nicolás
Harrand, Leonel
Marco, Martin Alberto
Masuh, Hector Mario
Validation of models to estimate the fumigant and larvicidal activity of Eucalyptus essential oils against Aedes aegypti (Diptera: Culicidae)
title Validation of models to estimate the fumigant and larvicidal activity of Eucalyptus essential oils against Aedes aegypti (Diptera: Culicidae)
title_full Validation of models to estimate the fumigant and larvicidal activity of Eucalyptus essential oils against Aedes aegypti (Diptera: Culicidae)
title_fullStr Validation of models to estimate the fumigant and larvicidal activity of Eucalyptus essential oils against Aedes aegypti (Diptera: Culicidae)
title_full_unstemmed Validation of models to estimate the fumigant and larvicidal activity of Eucalyptus essential oils against Aedes aegypti (Diptera: Culicidae)
title_short Validation of models to estimate the fumigant and larvicidal activity of Eucalyptus essential oils against Aedes aegypti (Diptera: Culicidae)
title_sort validation of models to estimate the fumigant and larvicidal activity of eucalyptus essential oils against aedes aegypti diptera culicidae
topic Aedes Aegypti
Control de Plagas
Aceites Esenciales
Eucalyptus
Larvicidas
Pest Control
Essential Oils
Larvicides
Mosquitos
Fiebre Amarilla
url https://link.springer.com/article/10.1007%2Fs00436-011-2685-9
http://hdl.handle.net/20.500.12123/4263
https://doi.org/10.1007/s00436-011-2685-9
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