Both Volatiles and Cuticular Plant Compounds Determine Oviposition of the Willow Sawfly Nematus oligospilus on Leaves of Salix spp. (Salicaceae)
Plant volatile organic compounds play a role in selection of host plants by herbivorous insects. Once the insect reaches the plant, contact cues determine host acceptance. Although the willow sawfly Nematus oligospilus (Hymenoptera: Tenthredinidae) can differentiate among willow genotypes, no knowle...
| Autores principales: | , , , , , , |
|---|---|
| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
Springer
2019
|
| Materias: | |
| Acceso en línea: | https://link.springer.com/article/10.1007%2Fs10886-015-0637-z http://hdl.handle.net/20.500.12123/4249 https://doi.org/10.1007/s10886-015-0637-z |
| _version_ | 1855035287230480384 |
|---|---|
| author | Braccini, Celina Laura Vega, Andrea Susana Coll Araoz, Maria Victoria Teal, Peter E. Cerrillo, Teresa Zavala, Jorge Alberto Fernandez, Patricia |
| author_browse | Braccini, Celina Laura Cerrillo, Teresa Coll Araoz, Maria Victoria Fernandez, Patricia Teal, Peter E. Vega, Andrea Susana Zavala, Jorge Alberto |
| author_facet | Braccini, Celina Laura Vega, Andrea Susana Coll Araoz, Maria Victoria Teal, Peter E. Cerrillo, Teresa Zavala, Jorge Alberto Fernandez, Patricia |
| author_sort | Braccini, Celina Laura |
| collection | INTA Digital |
| description | Plant volatile organic compounds play a role in selection of host plants by herbivorous insects. Once the insect reaches the plant, contact cues determine host acceptance. Although the willow sawfly Nematus oligospilus (Hymenoptera: Tenthredinidae) can differentiate among willow genotypes, no knowledge is available on the cues used by this insect to seek and accept the host plant. In this study, we recorded behavioral orientation in a Y-tube olfactometer of willow sawfly females to volatiles of the highly preferred genotype Salix nigra and the non-preferred genotype S. viminalis. The volatiles released by undamaged willows of each genotype were analyzed by coupled gas chromatography-mass spectrometry. Contact cues were evaluated first by oviposition preference bioassays after selective leaf wax removal, and then by studying the micromorphology of abaxial and adaxial leaf surfaces and their chemical composition. Willow sawfly females oriented preferentially to S. nigra volatiles, which contained more than 3 times the amount of volatiles than that collected from S. viminalis. Analysis of volatiles showed significant differences in amounts of (Z) and (E)-β-ocimene, undecane, decanal, and β-caryophyllene. The adaxial leaf surface of S. nigra was less preferred after wax removal, suggesting a role of cuticular waxes for oviposition acceptance. No differences were found among the micromorphology of leaf surfaces between preferred and non-preferred genotypes. The chemical analysis of cuticular waxes showed that the abaxial leaf surface of S. viminalis, which is completely avoided for oviposition, possessed 97 % of alkanes. The accepted leaf surfaces contained a more diverse wax profile including alcohols, acids, and esters. Thus, non-alkane wax compounds might be related to oviposition. In sum, our study suggests that several cues act in concert to provide oviposition cues for the sawfly N. oligospilus: females are attracted to volatiles from a distance, and once alighting on the plant, they seek specific chemical contact cues in order to lay eggs. |
| format | info:ar-repo/semantics/artículo |
| id | INTA4249 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA42492019-01-11T12:06:27Z Both Volatiles and Cuticular Plant Compounds Determine Oviposition of the Willow Sawfly Nematus oligospilus on Leaves of Salix spp. (Salicaceae) Braccini, Celina Laura Vega, Andrea Susana Coll Araoz, Maria Victoria Teal, Peter E. Cerrillo, Teresa Zavala, Jorge Alberto Fernandez, Patricia Nematus Salix Plagas de Plantas Oviposición Insecta Pests of Plants Oviposition Nematus oligospilus Sauce (salix) Compuestos Volátiles Plant volatile organic compounds play a role in selection of host plants by herbivorous insects. Once the insect reaches the plant, contact cues determine host acceptance. Although the willow sawfly Nematus oligospilus (Hymenoptera: Tenthredinidae) can differentiate among willow genotypes, no knowledge is available on the cues used by this insect to seek and accept the host plant. In this study, we recorded behavioral orientation in a Y-tube olfactometer of willow sawfly females to volatiles of the highly preferred genotype Salix nigra and the non-preferred genotype S. viminalis. The volatiles released by undamaged willows of each genotype were analyzed by coupled gas chromatography-mass spectrometry. Contact cues were evaluated first by oviposition preference bioassays after selective leaf wax removal, and then by studying the micromorphology of abaxial and adaxial leaf surfaces and their chemical composition. Willow sawfly females oriented preferentially to S. nigra volatiles, which contained more than 3 times the amount of volatiles than that collected from S. viminalis. Analysis of volatiles showed significant differences in amounts of (Z) and (E)-β-ocimene, undecane, decanal, and β-caryophyllene. The adaxial leaf surface of S. nigra was less preferred after wax removal, suggesting a role of cuticular waxes for oviposition acceptance. No differences were found among the micromorphology of leaf surfaces between preferred and non-preferred genotypes. The chemical analysis of cuticular waxes showed that the abaxial leaf surface of S. viminalis, which is completely avoided for oviposition, possessed 97 % of alkanes. The accepted leaf surfaces contained a more diverse wax profile including alcohols, acids, and esters. Thus, non-alkane wax compounds might be related to oviposition. In sum, our study suggests that several cues act in concert to provide oviposition cues for the sawfly N. oligospilus: females are attracted to volatiles from a distance, and once alighting on the plant, they seek specific chemical contact cues in order to lay eggs. Instituto de Recursos Biológicos Fil: Braccini, Celina Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina Fil: Vega, Andrea Susana. Universidad de Buenos Aires. Facultad de Agronomía. Cátedra de Botánica General; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Coll Araoz, Maria Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina Fil: Teal, Peter E. USDA-ARS. Center for Medical, Agricultural and Veterinary Entomology; Estados Unidos Fil: Cerrillo, Teresa. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Delta del Paraná; Argentina Fil: Zavala, Jorge Alberto. Universidad de Buenos Aires. Facultad de Agronomía. Cátedra de Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Fernandez, Patricia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Delta del Paraná; Argentina 2019-01-11T12:04:48Z 2019-01-11T12:04:48Z 2015-11 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://link.springer.com/article/10.1007%2Fs10886-015-0637-z http://hdl.handle.net/20.500.12123/4249 0098-0331 1573-1561 https://doi.org/10.1007/s10886-015-0637-z eng info:eu-repo/semantics/restrictedAccess application/pdf Springer Journal of Chemical Ecology 41 (11) : 985–996 (November 2015) |
| spellingShingle | Nematus Salix Plagas de Plantas Oviposición Insecta Pests of Plants Oviposition Nematus oligospilus Sauce (salix) Compuestos Volátiles Braccini, Celina Laura Vega, Andrea Susana Coll Araoz, Maria Victoria Teal, Peter E. Cerrillo, Teresa Zavala, Jorge Alberto Fernandez, Patricia Both Volatiles and Cuticular Plant Compounds Determine Oviposition of the Willow Sawfly Nematus oligospilus on Leaves of Salix spp. (Salicaceae) |
| title | Both Volatiles and Cuticular Plant Compounds Determine Oviposition of the Willow Sawfly Nematus oligospilus on Leaves of Salix spp. (Salicaceae) |
| title_full | Both Volatiles and Cuticular Plant Compounds Determine Oviposition of the Willow Sawfly Nematus oligospilus on Leaves of Salix spp. (Salicaceae) |
| title_fullStr | Both Volatiles and Cuticular Plant Compounds Determine Oviposition of the Willow Sawfly Nematus oligospilus on Leaves of Salix spp. (Salicaceae) |
| title_full_unstemmed | Both Volatiles and Cuticular Plant Compounds Determine Oviposition of the Willow Sawfly Nematus oligospilus on Leaves of Salix spp. (Salicaceae) |
| title_short | Both Volatiles and Cuticular Plant Compounds Determine Oviposition of the Willow Sawfly Nematus oligospilus on Leaves of Salix spp. (Salicaceae) |
| title_sort | both volatiles and cuticular plant compounds determine oviposition of the willow sawfly nematus oligospilus on leaves of salix spp salicaceae |
| topic | Nematus Salix Plagas de Plantas Oviposición Insecta Pests of Plants Oviposition Nematus oligospilus Sauce (salix) Compuestos Volátiles |
| url | https://link.springer.com/article/10.1007%2Fs10886-015-0637-z http://hdl.handle.net/20.500.12123/4249 https://doi.org/10.1007/s10886-015-0637-z |
| work_keys_str_mv | AT braccinicelinalaura bothvolatilesandcuticularplantcompoundsdetermineovipositionofthewillowsawflynematusoligospilusonleavesofsalixsppsalicaceae AT vegaandreasusana bothvolatilesandcuticularplantcompoundsdetermineovipositionofthewillowsawflynematusoligospilusonleavesofsalixsppsalicaceae AT collaraozmariavictoria bothvolatilesandcuticularplantcompoundsdetermineovipositionofthewillowsawflynematusoligospilusonleavesofsalixsppsalicaceae AT tealpetere bothvolatilesandcuticularplantcompoundsdetermineovipositionofthewillowsawflynematusoligospilusonleavesofsalixsppsalicaceae AT cerrilloteresa bothvolatilesandcuticularplantcompoundsdetermineovipositionofthewillowsawflynematusoligospilusonleavesofsalixsppsalicaceae AT zavalajorgealberto bothvolatilesandcuticularplantcompoundsdetermineovipositionofthewillowsawflynematusoligospilusonleavesofsalixsppsalicaceae AT fernandezpatricia bothvolatilesandcuticularplantcompoundsdetermineovipositionofthewillowsawflynematusoligospilusonleavesofsalixsppsalicaceae |