Pathogenicity of Nc-Bahia and Nc-1 strains of Neospora caninum in experimentally infected cows and buffaloes in early pregnancy
Neospora caninum is a protozoan parasite known as an important cause of bovine abortion worldwide. Little is currently known about how different strains of N. caninum vary in their pathogenicity. In this study, we compared a Brazilian strain, Nc-Bahia, with the first isolate of this coccidian, Nc-1....
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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Springer
2019
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| Online Access: | https://link.springer.com/article/10.1007/s00436-014-3796-x http://hdl.handle.net/20.500.12123/4225 https://doi.org/10.1007/s00436-014-3796-x |
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| author | Chryssafidis, Andreas Lazaros Canton, German Jose Chianini, Francesca Innes, Elisabeth A. Madureira, Ed Hoffmann Gennari, Solange Maria |
| author_browse | Canton, German Jose Chianini, Francesca Chryssafidis, Andreas Lazaros Gennari, Solange Maria Innes, Elisabeth A. Madureira, Ed Hoffmann |
| author_facet | Chryssafidis, Andreas Lazaros Canton, German Jose Chianini, Francesca Innes, Elisabeth A. Madureira, Ed Hoffmann Gennari, Solange Maria |
| author_sort | Chryssafidis, Andreas Lazaros |
| collection | INTA Digital |
| description | Neospora caninum is a protozoan parasite known as an important cause of bovine abortion worldwide. Little is currently known about how different strains of N. caninum vary in their pathogenicity. In this study, we compared a Brazilian strain, Nc-Bahia, with the first isolate of this coccidian, Nc-1. Eight cows and seven buffaloes were submitted to fixed-time artificial insemination protocols for a better control of pregnancy. Group 1 was inoculated with Nc-Bahia (n = 8; five cows and three buffaloes), and Group 2 was inoculated with Nc-1 (n = 5; two cows and three buffaloes). One nonpregnant female of each species was left uninfected as sentinel controls for potential environmental infection. All inoculated animals received 5 × 108 tachyzoites of N. caninum, by intravenous route, on the 70th day of gestation. Uninfected animals remained seronegative throughout the experiment, indicating no exogenous infection, whereas all inoculated animals became seropositive to N. caninum. In Group 1, abortion was found in only one cow on 42 days postinfection (dpi; frequency of abortion = 12.5 %), whilst all animals from Group 2 aborted on 35 dpi (frequency of abortion = 100 %). Parasite DNA was detected by seminested PCR in maternal, foetal and placental tissues, confirming vertical transmission in Groups 1 and 2, although histological lesions had different frequencies and degrees of severity between the groups. There was evidence of lower pathogenicity of Nc-Bahia compared to Nc-1 when used in experimental infection, as it caused fewer abortions, as well as less frequent and milder histological lesions. This was the first time Nc-Bahia has been used for experimental infection. |
| format | info:ar-repo/semantics/artículo |
| id | INTA4225 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA42252019-01-08T12:41:12Z Pathogenicity of Nc-Bahia and Nc-1 strains of Neospora caninum in experimentally infected cows and buffaloes in early pregnancy Chryssafidis, Andreas Lazaros Canton, German Jose Chianini, Francesca Innes, Elisabeth A. Madureira, Ed Hoffmann Gennari, Solange Maria Neospora caninum Enfermedades de los Animales Vaca Búfalo de Agua Gestación Patogenicidad Animal Diseases Cows Water Buffaloes Pregnancy Pathogenicity Búfalo (bubalus) Neospora caninum is a protozoan parasite known as an important cause of bovine abortion worldwide. Little is currently known about how different strains of N. caninum vary in their pathogenicity. In this study, we compared a Brazilian strain, Nc-Bahia, with the first isolate of this coccidian, Nc-1. Eight cows and seven buffaloes were submitted to fixed-time artificial insemination protocols for a better control of pregnancy. Group 1 was inoculated with Nc-Bahia (n = 8; five cows and three buffaloes), and Group 2 was inoculated with Nc-1 (n = 5; two cows and three buffaloes). One nonpregnant female of each species was left uninfected as sentinel controls for potential environmental infection. All inoculated animals received 5 × 108 tachyzoites of N. caninum, by intravenous route, on the 70th day of gestation. Uninfected animals remained seronegative throughout the experiment, indicating no exogenous infection, whereas all inoculated animals became seropositive to N. caninum. In Group 1, abortion was found in only one cow on 42 days postinfection (dpi; frequency of abortion = 12.5 %), whilst all animals from Group 2 aborted on 35 dpi (frequency of abortion = 100 %). Parasite DNA was detected by seminested PCR in maternal, foetal and placental tissues, confirming vertical transmission in Groups 1 and 2, although histological lesions had different frequencies and degrees of severity between the groups. There was evidence of lower pathogenicity of Nc-Bahia compared to Nc-1 when used in experimental infection, as it caused fewer abortions, as well as less frequent and milder histological lesions. This was the first time Nc-Bahia has been used for experimental infection. EEA Balcarce Fil: Chryssafidis, Andreas Lazaros. Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia. Departamento de Medicina Veterinária Preventiva e Saúde Animal; Brasil. University College Dublin. UCD School of Veterinary Medicine, Veterinary Science Centre; Irlanda Fil: Canton, German Jose. Moredun Research Institute; Reino Unido. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Chianini, Francesca. Moredun Research Institute; Reino Unido Fil: Innes, Elisabeth A. Moredun Research Institute; Reino Unido Fil: Madureira, Ed Hoffmann. Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia. Departamento de Reprodução Animal; Brasil Fil: Gennari, Solange Maria. Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia. Departamento de Medicina Veterinária Preventiva e Saúde Animal; Brasil 2019-01-08T12:38:21Z 2019-01-08T12:38:21Z 2014-04 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://link.springer.com/article/10.1007/s00436-014-3796-x http://hdl.handle.net/20.500.12123/4225 0932-0113 1432-1955 https://doi.org/10.1007/s00436-014-3796-x eng info:eu-repo/semantics/restrictedAccess application/pdf Springer Parasitology Research 113 (4) : 1521–1528 (April 2014) |
| spellingShingle | Neospora caninum Enfermedades de los Animales Vaca Búfalo de Agua Gestación Patogenicidad Animal Diseases Cows Water Buffaloes Pregnancy Pathogenicity Búfalo (bubalus) Chryssafidis, Andreas Lazaros Canton, German Jose Chianini, Francesca Innes, Elisabeth A. Madureira, Ed Hoffmann Gennari, Solange Maria Pathogenicity of Nc-Bahia and Nc-1 strains of Neospora caninum in experimentally infected cows and buffaloes in early pregnancy |
| title | Pathogenicity of Nc-Bahia and Nc-1 strains of Neospora caninum in experimentally infected cows and buffaloes in early pregnancy |
| title_full | Pathogenicity of Nc-Bahia and Nc-1 strains of Neospora caninum in experimentally infected cows and buffaloes in early pregnancy |
| title_fullStr | Pathogenicity of Nc-Bahia and Nc-1 strains of Neospora caninum in experimentally infected cows and buffaloes in early pregnancy |
| title_full_unstemmed | Pathogenicity of Nc-Bahia and Nc-1 strains of Neospora caninum in experimentally infected cows and buffaloes in early pregnancy |
| title_short | Pathogenicity of Nc-Bahia and Nc-1 strains of Neospora caninum in experimentally infected cows and buffaloes in early pregnancy |
| title_sort | pathogenicity of nc bahia and nc 1 strains of neospora caninum in experimentally infected cows and buffaloes in early pregnancy |
| topic | Neospora caninum Enfermedades de los Animales Vaca Búfalo de Agua Gestación Patogenicidad Animal Diseases Cows Water Buffaloes Pregnancy Pathogenicity Búfalo (bubalus) |
| url | https://link.springer.com/article/10.1007/s00436-014-3796-x http://hdl.handle.net/20.500.12123/4225 https://doi.org/10.1007/s00436-014-3796-x |
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