Characterization of a unique copper resistance gene cluster in Xanthomonas campestris pv. campestris isolated in Trinidad, West Indies
Whole genome sequencing of a copper resistant (CuR) black rot strain of Xanthomonas campestris pv. campestris (Xcc) isolated from a broccoli plant in Trinidad revealed a unique operon for copper resistance. The cop genes of strain Xcc-BrA1 were determined to be present on a 160 to 180 kb plasmid sho...
| Autores principales: | , , , , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
Springer
2018
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| Materias: | |
| Acceso en línea: | https://link.springer.com/article/10.1007/s10658-016-1035-2 http://hdl.handle.net/20.500.12123/4150 https://doi.org/10.1007/s10658-016-1035-2 |
| _version_ | 1855035270158614528 |
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| author | Behlau, Franklin Gochez, Alberto Martin Lugo, Alshia Janel Elibox, Winston Minsavage, Gerald V. Potnis, Neha White, Frank F. Ebrahim, Mohamed Jones, Jeffrey B. Ramsubhag, Adesh |
| author_browse | Behlau, Franklin Ebrahim, Mohamed Elibox, Winston Gochez, Alberto Martin Jones, Jeffrey B. Lugo, Alshia Janel Minsavage, Gerald V. Potnis, Neha Ramsubhag, Adesh White, Frank F. |
| author_facet | Behlau, Franklin Gochez, Alberto Martin Lugo, Alshia Janel Elibox, Winston Minsavage, Gerald V. Potnis, Neha White, Frank F. Ebrahim, Mohamed Jones, Jeffrey B. Ramsubhag, Adesh |
| author_sort | Behlau, Franklin |
| collection | INTA Digital |
| description | Whole genome sequencing of a copper resistant (CuR) black rot strain of Xanthomonas campestris pv. campestris (Xcc) isolated from a broccoli plant in Trinidad revealed a unique operon for copper resistance. The cop genes of strain Xcc-BrA1 were determined to be present on a 160 to 180 kb plasmid shown to be non-conjugative with other xanthomonads. While nucleotide comparison of a putative 8.0 Kbp copLABMGF gene cluster identified in Xcc-BrA1 genome did not reveal any homologous region with other known CuR Xanthomonas strains from diverse origins, the comparison of the translated amino acid sequence indicated similarity with X. citri, X. c. pv. citrumelonis and X. vesicatoria Cop proteins. Cloning of the copLAB gene cluster from Xcc-BrA1 conferred copper resistance to other copper-sensitive xanthomonads. Although Xcc-BrA1 harbors copLAB genes with similar sizes and organization and is able to grow on Cu-amended medium as other CuR xanthomonads, the phylogenetic analysis of nucleotide sequences indicates that the cop cluster in Xcc-BrA1 is unique and distantly related to other copLAB genes from Xanthomonas and Stenotrophomonas. The origin of copper resistance genes in Xcc-BrA1 is likely a result of horizontal gene acquisition from a still unknown phylloplane cohabitant. The findings of this study have implications for the management of crop diseases caused by CuR xanthomonads. Future studies could focus on and determining the distribution, overall importance and appropriate control measures for strains harbouring these unique genes. |
| format | info:ar-repo/semantics/artículo |
| id | INTA4150 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA41502018-12-26T16:56:51Z Characterization of a unique copper resistance gene cluster in Xanthomonas campestris pv. campestris isolated in Trinidad, West Indies Behlau, Franklin Gochez, Alberto Martin Lugo, Alshia Janel Elibox, Winston Minsavage, Gerald V. Potnis, Neha White, Frank F. Ebrahim, Mohamed Jones, Jeffrey B. Ramsubhag, Adesh Xanthomonas campestris Genética Enfermedades de las Plantas Cobre Filogenia Genomas Genetics Plant Diseases Copper Phylogeny Genomes Trinidad y Tobago Xanthomonas campestris pv. campestris Whole genome sequencing of a copper resistant (CuR) black rot strain of Xanthomonas campestris pv. campestris (Xcc) isolated from a broccoli plant in Trinidad revealed a unique operon for copper resistance. The cop genes of strain Xcc-BrA1 were determined to be present on a 160 to 180 kb plasmid shown to be non-conjugative with other xanthomonads. While nucleotide comparison of a putative 8.0 Kbp copLABMGF gene cluster identified in Xcc-BrA1 genome did not reveal any homologous region with other known CuR Xanthomonas strains from diverse origins, the comparison of the translated amino acid sequence indicated similarity with X. citri, X. c. pv. citrumelonis and X. vesicatoria Cop proteins. Cloning of the copLAB gene cluster from Xcc-BrA1 conferred copper resistance to other copper-sensitive xanthomonads. Although Xcc-BrA1 harbors copLAB genes with similar sizes and organization and is able to grow on Cu-amended medium as other CuR xanthomonads, the phylogenetic analysis of nucleotide sequences indicates that the cop cluster in Xcc-BrA1 is unique and distantly related to other copLAB genes from Xanthomonas and Stenotrophomonas. The origin of copper resistance genes in Xcc-BrA1 is likely a result of horizontal gene acquisition from a still unknown phylloplane cohabitant. The findings of this study have implications for the management of crop diseases caused by CuR xanthomonads. Future studies could focus on and determining the distribution, overall importance and appropriate control measures for strains harbouring these unique genes. EEA Bella Vista Fil: Behlau, Franklin. Fundo de Defesa da Citricultura. Pesquisa e Desenvolvimento; Brasil Fil: Gochez, Alberto Martin. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bella Vista; Argentina Fil: Lugo, Alshia Janel. University of the West Indies. Faculty of Science and Technology. Department of Life Sciences; Trinidad y Tobago Fil: Elibox, Winston. University of the West Indies. Faculty of Science and Technology. Department of Life Sciences; Trinidad y Tobago Fil: Minsavage, Gerald V. University of Florida. Department of Plant Pathology; Estados Unidos Fil: Potnis, Neha . University of Florida. Department of Plant Pathology; Estados Unidos Fil: White, Frank F. Kansas State University. Department of Plant Pathology; Estados Unidos Fil: Ebrahim, Mohamed. University of Florida. Department of Plant Pathology; Estados Unidos. Ain Shams University. Faculty of Agriculture. Department of Plant Pathology; Egipto Fil: Jones, Jeffrey B. University of Florida. Department of Plant Pathology; Estados Unidos Fil: Ramsubhag, Adesh. University of the West Indies. Faculty of Science and Technology. Department of Life Sciences; Trinidad y Tobago 2018-12-26T16:54:48Z 2018-12-26T16:54:48Z 2017-03 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://link.springer.com/article/10.1007/s10658-016-1035-2 http://hdl.handle.net/20.500.12123/4150 0929-1873 1573-8469 https://doi.org/10.1007/s10658-016-1035-2 eng info:eu-repo/semantics/restrictedAccess application/pdf Springer European Journal of Plant Pathology 147 (3) : 671–681 (March 2017) |
| spellingShingle | Xanthomonas campestris Genética Enfermedades de las Plantas Cobre Filogenia Genomas Genetics Plant Diseases Copper Phylogeny Genomes Trinidad y Tobago Xanthomonas campestris pv. campestris Behlau, Franklin Gochez, Alberto Martin Lugo, Alshia Janel Elibox, Winston Minsavage, Gerald V. Potnis, Neha White, Frank F. Ebrahim, Mohamed Jones, Jeffrey B. Ramsubhag, Adesh Characterization of a unique copper resistance gene cluster in Xanthomonas campestris pv. campestris isolated in Trinidad, West Indies |
| title | Characterization of a unique copper resistance gene cluster in Xanthomonas campestris pv. campestris isolated in Trinidad, West Indies |
| title_full | Characterization of a unique copper resistance gene cluster in Xanthomonas campestris pv. campestris isolated in Trinidad, West Indies |
| title_fullStr | Characterization of a unique copper resistance gene cluster in Xanthomonas campestris pv. campestris isolated in Trinidad, West Indies |
| title_full_unstemmed | Characterization of a unique copper resistance gene cluster in Xanthomonas campestris pv. campestris isolated in Trinidad, West Indies |
| title_short | Characterization of a unique copper resistance gene cluster in Xanthomonas campestris pv. campestris isolated in Trinidad, West Indies |
| title_sort | characterization of a unique copper resistance gene cluster in xanthomonas campestris pv campestris isolated in trinidad west indies |
| topic | Xanthomonas campestris Genética Enfermedades de las Plantas Cobre Filogenia Genomas Genetics Plant Diseases Copper Phylogeny Genomes Trinidad y Tobago Xanthomonas campestris pv. campestris |
| url | https://link.springer.com/article/10.1007/s10658-016-1035-2 http://hdl.handle.net/20.500.12123/4150 https://doi.org/10.1007/s10658-016-1035-2 |
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