Multiclass Compatible Sample Preparation for UHPLC–MS/MS Determination of Aflatoxin M1 in Raw Milk
A sample preparation method for aflatoxin M1 (AFM1) determination in raw milk was optimized following the quick, easy, cheap, effective, rugged and safe (QuEChERS) strategy, as an alternative to the classic immunoaffinity column clean-up (IAC). The method was adapted to address the complexity of the...
| Autores principales: | , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
Springer
2018
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| Materias: | |
| Acceso en línea: | https://link.springer.com/article/10.1007/s10337-015-2972-1 http://hdl.handle.net/20.500.12123/4095 https://doi.org/10.1007/s10337-015-2972-1 |
| _version_ | 1855035259483062272 |
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| author | Michlig, Nicolás Repetti, María Rosa Chiericatti, Carolina García, Silvia R. Gaggiotti, Monica Del Carmen Basílico, Juan Carlos Beldoménico, Horacio Ramón |
| author_browse | Basílico, Juan Carlos Beldoménico, Horacio Ramón Chiericatti, Carolina Gaggiotti, Monica Del Carmen García, Silvia R. Michlig, Nicolás Repetti, María Rosa |
| author_facet | Michlig, Nicolás Repetti, María Rosa Chiericatti, Carolina García, Silvia R. Gaggiotti, Monica Del Carmen Basílico, Juan Carlos Beldoménico, Horacio Ramón |
| author_sort | Michlig, Nicolás |
| collection | INTA Digital |
| description | A sample preparation method for aflatoxin M1 (AFM1) determination in raw milk was optimized following the quick, easy, cheap, effective, rugged and safe (QuEChERS) strategy, as an alternative to the classic immunoaffinity column clean-up (IAC). The method was adapted to address the complexity of the milk matrix, and to be suitable for final determination by ultra-high performance liquid chromatography coupled to tandem mass spectrometry (UHPLC–MS/MS). This approach proved also to be compatible with the simultaneous extraction of pesticide residues and other contaminants (mycotoxins). Regarding AFM1, satisfactory linearity was achieved and appropriate sensitivity was maintained, using matrix-matched calibration to compensate for the heavy ion suppression. The accuracy and precision, which were determined through recovery studies, were 70–95 %, with the relative standard deviation below 15 % in all of the cases. The limit of detection (LOD, 0.002 μg L−1) and limit of quantification (0.007 μg L−1) are compatible with current worldwide regulations (maximum levels of 0.5 and 0.05 μg L−1). The procedure was applied to samples that were naturally contaminated with a range of AFM1 at LOQ–0.187 μg L−1, with comparable results to IAC clean-up, which was employed as a reference method. Therefore, AFM1 determination in raw milk by UHPLC–MS/MS detection through the present QuEChERS extraction constitutes a reliable alternative to IAC clean-up and exhibits advantages related to cost, accessibility of materials and simplicity of operation. |
| format | info:ar-repo/semantics/artículo |
| id | INTA4095 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA40952019-09-11T18:38:40Z Multiclass Compatible Sample Preparation for UHPLC–MS/MS Determination of Aflatoxin M1 in Raw Milk Michlig, Nicolás Repetti, María Rosa Chiericatti, Carolina García, Silvia R. Gaggiotti, Monica Del Carmen Basílico, Juan Carlos Beldoménico, Horacio Ramón Leche Cruda Aflatoxinas Espectrometría de Masas Micotoxinas Plaguicidas Raw Milk Aflatoxins Mass Spectrometry Mycotoxins Pesticides A sample preparation method for aflatoxin M1 (AFM1) determination in raw milk was optimized following the quick, easy, cheap, effective, rugged and safe (QuEChERS) strategy, as an alternative to the classic immunoaffinity column clean-up (IAC). The method was adapted to address the complexity of the milk matrix, and to be suitable for final determination by ultra-high performance liquid chromatography coupled to tandem mass spectrometry (UHPLC–MS/MS). This approach proved also to be compatible with the simultaneous extraction of pesticide residues and other contaminants (mycotoxins). Regarding AFM1, satisfactory linearity was achieved and appropriate sensitivity was maintained, using matrix-matched calibration to compensate for the heavy ion suppression. The accuracy and precision, which were determined through recovery studies, were 70–95 %, with the relative standard deviation below 15 % in all of the cases. The limit of detection (LOD, 0.002 μg L−1) and limit of quantification (0.007 μg L−1) are compatible with current worldwide regulations (maximum levels of 0.5 and 0.05 μg L−1). The procedure was applied to samples that were naturally contaminated with a range of AFM1 at LOQ–0.187 μg L−1, with comparable results to IAC clean-up, which was employed as a reference method. Therefore, AFM1 determination in raw milk by UHPLC–MS/MS detection through the present QuEChERS extraction constitutes a reliable alternative to IAC clean-up and exhibits advantages related to cost, accessibility of materials and simplicity of operation. EEA Rafaela Fil: Michlig, Nicolás. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Programa de Investigación y Análisis de Residuos y Contaminantes Químicos; Argentina. Fil: Repetti, María Rosa. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Programa de Investigación y Análisis de Residuos y Contaminantes Químicos (PRINARC); Argentina Fil: Chiericatti, Carolina. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Laboratorio de Microbiología; Argentina Fil: García, Silvia R.. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Programa de Investigación y Análisis de Residuos y Contaminantes Químicos; Argentina Fil: Gaggiotti, Monica Del Carmen. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina Fil: Basílico, Juan Carlos. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Laboratorio de Microbiología; Argentina Fil: Beldoménico, Horacio Ramón. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Programa de Investigación y Análisis de Residuos y Contaminantes Químicos (PRINARC); Argentina 2018-12-17T11:42:49Z 2018-12-17T11:42:49Z 2016-09 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://link.springer.com/article/10.1007/s10337-015-2972-1 http://hdl.handle.net/20.500.12123/4095 0009-5893 1612-1112 https://doi.org/10.1007/s10337-015-2972-1 eng info:eu-repo/semantics/restrictedAccess application/pdf Springer Chromatographia 79 (17–18) : 1091–1100 (September 2016) |
| spellingShingle | Leche Cruda Aflatoxinas Espectrometría de Masas Micotoxinas Plaguicidas Raw Milk Aflatoxins Mass Spectrometry Mycotoxins Pesticides Michlig, Nicolás Repetti, María Rosa Chiericatti, Carolina García, Silvia R. Gaggiotti, Monica Del Carmen Basílico, Juan Carlos Beldoménico, Horacio Ramón Multiclass Compatible Sample Preparation for UHPLC–MS/MS Determination of Aflatoxin M1 in Raw Milk |
| title | Multiclass Compatible Sample Preparation for UHPLC–MS/MS Determination of Aflatoxin M1 in Raw Milk |
| title_full | Multiclass Compatible Sample Preparation for UHPLC–MS/MS Determination of Aflatoxin M1 in Raw Milk |
| title_fullStr | Multiclass Compatible Sample Preparation for UHPLC–MS/MS Determination of Aflatoxin M1 in Raw Milk |
| title_full_unstemmed | Multiclass Compatible Sample Preparation for UHPLC–MS/MS Determination of Aflatoxin M1 in Raw Milk |
| title_short | Multiclass Compatible Sample Preparation for UHPLC–MS/MS Determination of Aflatoxin M1 in Raw Milk |
| title_sort | multiclass compatible sample preparation for uhplc ms ms determination of aflatoxin m1 in raw milk |
| topic | Leche Cruda Aflatoxinas Espectrometría de Masas Micotoxinas Plaguicidas Raw Milk Aflatoxins Mass Spectrometry Mycotoxins Pesticides |
| url | https://link.springer.com/article/10.1007/s10337-015-2972-1 http://hdl.handle.net/20.500.12123/4095 https://doi.org/10.1007/s10337-015-2972-1 |
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